Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03595 and HMPREF1058_RS03590 are separated by 50 nucleotides HMPREF1058_RS03590 and HMPREF1058_RS03585 are separated by 654 nucleotides
HMPREF1058_RS03595: HMPREF1058_RS03595 - hypothetical protein, at 241,655 to 242,494
_RS03595
HMPREF1058_RS03590: HMPREF1058_RS03590 - hypothetical protein, at 242,545 to 243,912
_RS03590
HMPREF1058_RS03585: HMPREF1058_RS03585 - hypothetical protein, at 244,567 to 244,950
_RS03585
Position (kb)
242
243
244 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6 at 241.560 kb on - strand at 241.565 kb on + strand at 241.565 kb on + strand at 241.565 kb on + strand at 241.565 kb on + strand at 241.566 kb on - strand at 241.566 kb on - strand at 241.585 kb on + strand at 241.586 kb on - strand at 241.586 kb on - strand at 241.596 kb on - strand at 241.599 kb on - strand at 241.605 kb on - strand at 241.632 kb on + strand at 241.633 kb on - strand at 241.633 kb on - strand at 241.633 kb on - strand at 241.633 kb on - strand at 241.633 kb on - strand at 241.657 kb on - strand at 241.701 kb on - strand at 241.785 kb on - strand, within HMPREF1058_RS03595 at 241.815 kb on - strand, within HMPREF1058_RS03595 at 241.815 kb on - strand, within HMPREF1058_RS03595 at 241.815 kb on - strand, within HMPREF1058_RS03595 at 241.818 kb on + strand, within HMPREF1058_RS03595 at 241.818 kb on + strand at 241.824 kb on - strand, within HMPREF1058_RS03595 at 241.865 kb on + strand, within HMPREF1058_RS03595 at 241.999 kb on - strand, within HMPREF1058_RS03595 at 242.000 kb on + strand, within HMPREF1058_RS03595 at 242.001 kb on - strand, within HMPREF1058_RS03595 at 242.001 kb on - strand, within HMPREF1058_RS03595 at 242.001 kb on - strand, within HMPREF1058_RS03595 at 242.002 kb on + strand, within HMPREF1058_RS03595 at 242.003 kb on - strand, within HMPREF1058_RS03595 at 242.003 kb on - strand, within HMPREF1058_RS03595 at 242.033 kb on + strand, within HMPREF1058_RS03595 at 242.050 kb on + strand, within HMPREF1058_RS03595 at 242.078 kb on + strand, within HMPREF1058_RS03595 at 242.079 kb on - strand, within HMPREF1058_RS03595 at 242.079 kb on - strand at 242.080 kb on + strand, within HMPREF1058_RS03595 at 242.080 kb on + strand, within HMPREF1058_RS03595 at 242.081 kb on - strand, within HMPREF1058_RS03595 at 242.081 kb on - strand, within HMPREF1058_RS03595 at 242.204 kb on + strand, within HMPREF1058_RS03595 at 242.204 kb on + strand, within HMPREF1058_RS03595 at 242.207 kb on - strand, within HMPREF1058_RS03595 at 242.266 kb on - strand, within HMPREF1058_RS03595 at 242.311 kb on + strand, within HMPREF1058_RS03595 at 242.321 kb on + strand, within HMPREF1058_RS03595 at 242.321 kb on + strand, within HMPREF1058_RS03595 at 242.394 kb on - strand, within HMPREF1058_RS03595 at 242.416 kb on + strand at 242.417 kb on - strand at 242.421 kb on - strand at 242.424 kb on + strand at 242.425 kb on - strand at 242.425 kb on - strand at 242.484 kb on + strand at 242.484 kb on + strand at 242.484 kb on + strand at 242.485 kb on - strand at 242.537 kb on + strand at 242.537 kb on + strand at 242.597 kb on - strand at 242.767 kb on - strand, within HMPREF1058_RS03590 at 242.768 kb on + strand, within HMPREF1058_RS03590 at 242.972 kb on + strand, within HMPREF1058_RS03590 at 242.973 kb on - strand, within HMPREF1058_RS03590 at 242.973 kb on - strand, within HMPREF1058_RS03590 at 243.024 kb on + strand, within HMPREF1058_RS03590 at 243.028 kb on + strand, within HMPREF1058_RS03590 at 243.028 kb on + strand, within HMPREF1058_RS03590 at 243.029 kb on - strand, within HMPREF1058_RS03590 at 243.045 kb on + strand, within HMPREF1058_RS03590 at 243.046 kb on - strand, within HMPREF1058_RS03590 at 243.054 kb on + strand, within HMPREF1058_RS03590 at 243.171 kb on + strand, within HMPREF1058_RS03590 at 243.202 kb on + strand, within HMPREF1058_RS03590 at 243.214 kb on + strand, within HMPREF1058_RS03590 at 243.214 kb on + strand, within HMPREF1058_RS03590 at 243.255 kb on + strand, within HMPREF1058_RS03590 at 243.270 kb on + strand, within HMPREF1058_RS03590 at 243.270 kb on + strand, within HMPREF1058_RS03590 at 243.271 kb on - strand, within HMPREF1058_RS03590 at 243.338 kb on + strand, within HMPREF1058_RS03590 at 243.420 kb on + strand, within HMPREF1058_RS03590 at 243.420 kb on + strand, within HMPREF1058_RS03590 at 243.495 kb on + strand, within HMPREF1058_RS03590 at 243.510 kb on + strand, within HMPREF1058_RS03590 at 243.515 kb on - strand, within HMPREF1058_RS03590 at 243.519 kb on + strand, within HMPREF1058_RS03590 at 243.562 kb on + strand, within HMPREF1058_RS03590 at 243.596 kb on + strand, within HMPREF1058_RS03590 at 243.596 kb on + strand, within HMPREF1058_RS03590 at 243.596 kb on + strand, within HMPREF1058_RS03590 at 243.596 kb on + strand, within HMPREF1058_RS03590 at 243.596 kb on + strand, within HMPREF1058_RS03590 at 243.596 kb on + strand, within HMPREF1058_RS03590 at 243.597 kb on - strand, within HMPREF1058_RS03590 at 243.597 kb on - strand, within HMPREF1058_RS03590 at 243.601 kb on - strand, within HMPREF1058_RS03590 at 243.603 kb on + strand, within HMPREF1058_RS03590 at 243.604 kb on - strand, within HMPREF1058_RS03590 at 243.612 kb on - strand, within HMPREF1058_RS03590 at 243.663 kb on + strand, within HMPREF1058_RS03590 at 243.802 kb on + strand at 243.803 kb on - strand at 243.804 kb on + strand at 243.853 kb on - strand at 243.875 kb on + strand at 243.875 kb on + strand at 243.897 kb on + strand at 243.898 kb on - strand at 243.898 kb on - strand at 243.900 kb on - strand at 243.913 kb on - strand at 243.918 kb on + strand at 243.927 kb on - strand at 243.968 kb on - strand at 243.987 kb on + strand at 244.509 kb on + strand at 244.509 kb on + strand at 244.510 kb on - strand at 244.510 kb on - strand at 244.671 kb on + strand, within HMPREF1058_RS03585 at 244.672 kb on - strand, within HMPREF1058_RS03585 at 244.676 kb on + strand, within HMPREF1058_RS03585 at 244.676 kb on + strand, within HMPREF1058_RS03585 at 244.730 kb on + strand, within HMPREF1058_RS03585 at 244.889 kb on + strand, within HMPREF1058_RS03585
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 241,560 - +3.7 241,565 + +1.2 241,565 + +2.9 241,565 + +1.3 241,565 + -2.0 241,566 - +4.4 241,566 - +5.2 241,585 + +0.8 241,586 - +0.9 241,586 - -0.3 241,596 - +4.2 241,599 - +4.4 241,605 - +0.5 241,632 + -0.1 241,633 - +0.4 241,633 - +0.9 241,633 - +4.2 241,633 - -0.1 241,633 - +2.9 241,657 - +3.8 241,701 - +4.7 241,785 - HMPREF1058_RS03595 0.15 +6.5 241,815 - HMPREF1058_RS03595 0.19 +2.1 241,815 - HMPREF1058_RS03595 0.19 +2.4 241,815 - HMPREF1058_RS03595 0.19 +0.5 241,818 + HMPREF1058_RS03595 0.19 -0.8 241,818 + +2.5 241,824 - HMPREF1058_RS03595 0.20 +3.6 241,865 + HMPREF1058_RS03595 0.25 +0.2 241,999 - HMPREF1058_RS03595 0.41 -0.3 242,000 + HMPREF1058_RS03595 0.41 +1.0 242,001 - HMPREF1058_RS03595 0.41 +1.9 242,001 - HMPREF1058_RS03595 0.41 +2.8 242,001 - HMPREF1058_RS03595 0.41 +1.5 242,002 + HMPREF1058_RS03595 0.41 -0.1 242,003 - HMPREF1058_RS03595 0.41 +0.7 242,003 - HMPREF1058_RS03595 0.41 +2.8 242,033 + HMPREF1058_RS03595 0.45 -1.2 242,050 + HMPREF1058_RS03595 0.47 +1.5 242,078 + HMPREF1058_RS03595 0.50 +2.0 242,079 - HMPREF1058_RS03595 0.50 +1.4 242,079 - +0.9 242,080 + HMPREF1058_RS03595 0.51 -0.5 242,080 + HMPREF1058_RS03595 0.51 -1.3 242,081 - HMPREF1058_RS03595 0.51 +0.9 242,081 - HMPREF1058_RS03595 0.51 -0.2 242,204 + HMPREF1058_RS03595 0.65 -0.1 242,204 + HMPREF1058_RS03595 0.65 -0.1 242,207 - HMPREF1058_RS03595 0.66 +0.5 242,266 - HMPREF1058_RS03595 0.73 +3.1 242,311 + HMPREF1058_RS03595 0.78 +0.1 242,321 + HMPREF1058_RS03595 0.79 +1.9 242,321 + HMPREF1058_RS03595 0.79 -1.5 242,394 - HMPREF1058_RS03595 0.88 +2.9 242,416 + -0.5 242,417 - +1.1 242,421 - +1.0 242,424 + -0.2 242,425 - -1.1 242,425 - +2.6 242,484 + +1.5 242,484 + +0.2 242,484 + -0.1 242,485 - +0.9 242,537 + -1.2 242,537 + -0.3 242,597 - +3.1 242,767 - HMPREF1058_RS03590 0.16 +4.1 242,768 + HMPREF1058_RS03590 0.16 +0.5 242,972 + HMPREF1058_RS03590 0.31 +0.1 242,973 - HMPREF1058_RS03590 0.31 +3.2 242,973 - HMPREF1058_RS03590 0.31 +1.1 243,024 + HMPREF1058_RS03590 0.35 +2.2 243,028 + HMPREF1058_RS03590 0.35 -2.2 243,028 + HMPREF1058_RS03590 0.35 +0.7 243,029 - HMPREF1058_RS03590 0.35 +2.8 243,045 + HMPREF1058_RS03590 0.37 -1.1 243,046 - HMPREF1058_RS03590 0.37 +2.7 243,054 + HMPREF1058_RS03590 0.37 -0.8 243,171 + HMPREF1058_RS03590 0.46 +0.6 243,202 + HMPREF1058_RS03590 0.48 +0.2 243,214 + HMPREF1058_RS03590 0.49 -2.0 243,214 + HMPREF1058_RS03590 0.49 -2.0 243,255 + HMPREF1058_RS03590 0.52 +2.2 243,270 + HMPREF1058_RS03590 0.53 +4.1 243,270 + HMPREF1058_RS03590 0.53 -0.3 243,271 - HMPREF1058_RS03590 0.53 +2.5 243,338 + HMPREF1058_RS03590 0.58 +0.6 243,420 + HMPREF1058_RS03590 0.64 +5.5 243,420 + HMPREF1058_RS03590 0.64 -0.4 243,495 + HMPREF1058_RS03590 0.69 -0.6 243,510 + HMPREF1058_RS03590 0.71 -1.8 243,515 - HMPREF1058_RS03590 0.71 -0.5 243,519 + HMPREF1058_RS03590 0.71 +0.5 243,562 + HMPREF1058_RS03590 0.74 +2.1 243,596 + HMPREF1058_RS03590 0.77 -2.3 243,596 + HMPREF1058_RS03590 0.77 -0.1 243,596 + HMPREF1058_RS03590 0.77 +0.8 243,596 + HMPREF1058_RS03590 0.77 +0.5 243,596 + HMPREF1058_RS03590 0.77 +0.6 243,596 + HMPREF1058_RS03590 0.77 -0.9 243,597 - HMPREF1058_RS03590 0.77 +2.4 243,597 - HMPREF1058_RS03590 0.77 +1.6 243,601 - HMPREF1058_RS03590 0.77 -0.1 243,603 + HMPREF1058_RS03590 0.77 +2.2 243,604 - HMPREF1058_RS03590 0.77 +2.3 243,612 - HMPREF1058_RS03590 0.78 +5.3 243,663 + HMPREF1058_RS03590 0.82 +1.2 243,802 + -2.5 243,803 - +0.5 243,804 + +0.5 243,853 - +2.2 243,875 + +0.8 243,875 + -0.6 243,897 + +1.5 243,898 - -0.3 243,898 - +0.7 243,900 - +3.7 243,913 - -1.8 243,918 + -0.9 243,927 - +0.2 243,968 - -2.2 243,987 + +1.5 244,509 + -2.1 244,509 + -0.5 244,510 - -2.3 244,510 - +1.3 244,671 + HMPREF1058_RS03585 0.27 +0.1 244,672 - HMPREF1058_RS03585 0.27 -0.2 244,676 + HMPREF1058_RS03585 0.28 -1.3 244,676 + HMPREF1058_RS03585 0.28 -1.1 244,730 + HMPREF1058_RS03585 0.42 +1.2 244,889 + HMPREF1058_RS03585 0.84 -0.9
Or see this region's nucleotide sequence