Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06475 and HMPREF1058_RS0119800 are separated by 551 nucleotides HMPREF1058_RS0119800 and HMPREF1058_RS06470 are separated by 388 nucleotides HMPREF1058_RS06470 and HMPREF1058_RS20890 are separated by 170 nucleotides
HMPREF1058_RS06475: HMPREF1058_RS06475 - DUF4373 domain-containing protein, at 468,730 to 470,214
_RS06475
HMPREF1058_RS0119800: HMPREF1058_RS0119800 - toprim domain-containing protein, at 470,766 to 472,289
_RS0119800
HMPREF1058_RS06470: HMPREF1058_RS06470 - hypothetical protein, at 472,678 to 472,992
_RS06470
HMPREF1058_RS20890: HMPREF1058_RS20890 - N-acetylmuramoyl-L-alanine amidase, at 473,163 to 473,579
_RS20890
Position (kb) 470
471
472
473 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 470.101 kb on + strand at 470.346 kb on - strand at 470.548 kb on + strand at 470.549 kb on - strand at 470.730 kb on + strand at 470.734 kb on + strand at 470.788 kb on - strand at 470.996 kb on - strand, within HMPREF1058_RS0119800 at 471.291 kb on + strand, within HMPREF1058_RS0119800 at 471.401 kb on + strand, within HMPREF1058_RS0119800 at 471.867 kb on + strand, within HMPREF1058_RS0119800 at 471.868 kb on - strand, within HMPREF1058_RS0119800 at 471.868 kb on - strand, within HMPREF1058_RS0119800 at 472.099 kb on + strand, within HMPREF1058_RS0119800 at 472.196 kb on + strand at 472.275 kb on - strand at 472.372 kb on + strand at 472.381 kb on + strand at 472.382 kb on - strand at 472.385 kb on + strand at 472.386 kb on - strand at 472.387 kb on + strand at 472.394 kb on + strand at 472.406 kb on - strand at 472.431 kb on - strand at 472.526 kb on + strand at 472.777 kb on - strand, within HMPREF1058_RS06470 at 472.846 kb on + strand, within HMPREF1058_RS06470 at 472.847 kb on - strand, within HMPREF1058_RS06470 at 472.847 kb on - strand, within HMPREF1058_RS06470 at 472.946 kb on + strand, within HMPREF1058_RS06470 at 472.986 kb on - strand at 473.019 kb on - strand at 473.063 kb on + strand at 473.063 kb on + strand at 473.089 kb on - strand at 473.236 kb on - strand, within HMPREF1058_RS20890 at 473.286 kb on - strand, within HMPREF1058_RS20890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 470,101 + +2.0 470,346 - -0.8 470,548 + -1.5 470,549 - +0.4 470,730 + -3.2 470,734 + -0.3 470,788 - +0.3 470,996 - HMPREF1058_RS0119800 0.15 -1.5 471,291 + HMPREF1058_RS0119800 0.34 -0.2 471,401 + HMPREF1058_RS0119800 0.42 -1.0 471,867 + HMPREF1058_RS0119800 0.72 -1.9 471,868 - HMPREF1058_RS0119800 0.72 -0.2 471,868 - HMPREF1058_RS0119800 0.72 -0.5 472,099 + HMPREF1058_RS0119800 0.87 -0.9 472,196 + -0.9 472,275 - +0.5 472,372 + +0.2 472,381 + -0.4 472,382 - -0.5 472,385 + -0.5 472,386 - +0.2 472,387 + -1.7 472,394 + +0.1 472,406 - -2.8 472,431 - +0.2 472,526 + -1.2 472,777 - HMPREF1058_RS06470 0.31 +0.3 472,846 + HMPREF1058_RS06470 0.53 +1.2 472,847 - HMPREF1058_RS06470 0.54 +2.3 472,847 - HMPREF1058_RS06470 0.54 -0.0 472,946 + HMPREF1058_RS06470 0.85 -1.2 472,986 - +1.4 473,019 - -0.8 473,063 + +0.4 473,063 + +0.1 473,089 - -2.9 473,236 - HMPREF1058_RS20890 0.18 +0.5 473,286 - HMPREF1058_RS20890 0.29 -0.8
Or see this region's nucleotide sequence