Experiment: L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02770 and HMPREF1058_RS02765 are separated by 93 nucleotides HMPREF1058_RS02765 and HMPREF1058_RS02760 are separated by 12 nucleotides
HMPREF1058_RS02770: HMPREF1058_RS02770 - MATE family efflux transporter, at 97,643 to 98,953
_RS02770
HMPREF1058_RS02765: HMPREF1058_RS02765 - insulinase family protein, at 99,047 to 100,291
_RS02765
HMPREF1058_RS02760: HMPREF1058_RS02760 - peptidylprolyl isomerase, at 100,304 to 101,149
_RS02760
Position (kb)
99
100
101 Strain fitness (log2 ratio)
-1
0
1
2
3 at 98.106 kb on + strand, within HMPREF1058_RS02770 at 98.107 kb on - strand, within HMPREF1058_RS02770 at 98.107 kb on - strand, within HMPREF1058_RS02770 at 98.164 kb on + strand, within HMPREF1058_RS02770 at 98.284 kb on + strand, within HMPREF1058_RS02770 at 98.311 kb on + strand, within HMPREF1058_RS02770 at 98.312 kb on - strand, within HMPREF1058_RS02770 at 98.312 kb on - strand, within HMPREF1058_RS02770 at 98.442 kb on + strand, within HMPREF1058_RS02770 at 98.478 kb on - strand, within HMPREF1058_RS02770 at 98.561 kb on + strand, within HMPREF1058_RS02770 at 98.643 kb on - strand, within HMPREF1058_RS02770 at 98.804 kb on - strand, within HMPREF1058_RS02770 at 98.804 kb on - strand, within HMPREF1058_RS02770 at 98.897 kb on + strand at 98.897 kb on + strand at 98.925 kb on - strand at 98.944 kb on - strand at 98.985 kb on - strand at 99.038 kb on - strand at 99.043 kb on - strand at 99.067 kb on + strand at 99.067 kb on + strand at 99.069 kb on + strand at 99.072 kb on + strand at 99.073 kb on - strand at 99.156 kb on - strand at 99.415 kb on + strand, within HMPREF1058_RS02765 at 99.416 kb on - strand, within HMPREF1058_RS02765 at 99.417 kb on + strand, within HMPREF1058_RS02765 at 99.418 kb on - strand, within HMPREF1058_RS02765 at 99.438 kb on - strand, within HMPREF1058_RS02765 at 99.504 kb on - strand, within HMPREF1058_RS02765 at 99.520 kb on - strand, within HMPREF1058_RS02765 at 99.712 kb on + strand, within HMPREF1058_RS02765 at 99.713 kb on - strand, within HMPREF1058_RS02765 at 99.753 kb on + strand, within HMPREF1058_RS02765 at 99.847 kb on + strand, within HMPREF1058_RS02765 at 99.847 kb on + strand, within HMPREF1058_RS02765 at 99.847 kb on + strand, within HMPREF1058_RS02765 at 99.848 kb on - strand, within HMPREF1058_RS02765 at 99.849 kb on + strand, within HMPREF1058_RS02765 at 99.849 kb on + strand, within HMPREF1058_RS02765 at 99.976 kb on + strand, within HMPREF1058_RS02765 at 99.977 kb on - strand, within HMPREF1058_RS02765 at 100.032 kb on + strand, within HMPREF1058_RS02765 at 100.044 kb on - strand, within HMPREF1058_RS02765 at 100.197 kb on + strand at 100.228 kb on + strand at 100.245 kb on - strand at 100.313 kb on - strand at 100.313 kb on - strand at 100.370 kb on + strand at 100.388 kb on + strand at 100.423 kb on + strand, within HMPREF1058_RS02760 at 100.425 kb on + strand, within HMPREF1058_RS02760 at 100.465 kb on - strand, within HMPREF1058_RS02760 at 100.547 kb on + strand, within HMPREF1058_RS02760 at 100.547 kb on + strand, within HMPREF1058_RS02760 at 100.591 kb on - strand, within HMPREF1058_RS02760 at 100.593 kb on - strand, within HMPREF1058_RS02760 at 100.652 kb on + strand, within HMPREF1058_RS02760 at 100.710 kb on + strand, within HMPREF1058_RS02760 at 100.710 kb on + strand, within HMPREF1058_RS02760 at 100.711 kb on - strand, within HMPREF1058_RS02760 at 100.936 kb on - strand, within HMPREF1058_RS02760 at 100.937 kb on + strand, within HMPREF1058_RS02760 at 100.938 kb on - strand, within HMPREF1058_RS02760 at 100.991 kb on - strand, within HMPREF1058_RS02760 at 100.992 kb on + strand, within HMPREF1058_RS02760 at 101.124 kb on - strand at 101.127 kb on - strand at 101.197 kb on + strand at 101.251 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Asparagine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 98,106 + HMPREF1058_RS02770 0.35 -0.4 98,107 - HMPREF1058_RS02770 0.35 -0.8 98,107 - HMPREF1058_RS02770 0.35 -0.2 98,164 + HMPREF1058_RS02770 0.40 -0.1 98,284 + HMPREF1058_RS02770 0.49 +1.2 98,311 + HMPREF1058_RS02770 0.51 -0.6 98,312 - HMPREF1058_RS02770 0.51 +0.4 98,312 - HMPREF1058_RS02770 0.51 -0.9 98,442 + HMPREF1058_RS02770 0.61 +2.2 98,478 - HMPREF1058_RS02770 0.64 -0.1 98,561 + HMPREF1058_RS02770 0.70 -0.2 98,643 - HMPREF1058_RS02770 0.76 +0.7 98,804 - HMPREF1058_RS02770 0.89 -0.6 98,804 - HMPREF1058_RS02770 0.89 -0.4 98,897 + -0.5 98,897 + -1.1 98,925 - -0.2 98,944 - +1.1 98,985 - -0.1 99,038 - -0.2 99,043 - -0.2 99,067 + +2.4 99,067 + -0.9 99,069 + +2.0 99,072 + +0.5 99,073 - +1.0 99,156 - +0.9 99,415 + HMPREF1058_RS02765 0.30 +1.4 99,416 - HMPREF1058_RS02765 0.30 +1.4 99,417 + HMPREF1058_RS02765 0.30 +1.5 99,418 - HMPREF1058_RS02765 0.30 +0.9 99,438 - HMPREF1058_RS02765 0.31 +0.2 99,504 - HMPREF1058_RS02765 0.37 +0.9 99,520 - HMPREF1058_RS02765 0.38 +0.9 99,712 + HMPREF1058_RS02765 0.53 +1.7 99,713 - HMPREF1058_RS02765 0.53 +1.2 99,753 + HMPREF1058_RS02765 0.57 +1.3 99,847 + HMPREF1058_RS02765 0.64 +1.2 99,847 + HMPREF1058_RS02765 0.64 +1.4 99,847 + HMPREF1058_RS02765 0.64 +1.2 99,848 - HMPREF1058_RS02765 0.64 +0.6 99,849 + HMPREF1058_RS02765 0.64 +1.3 99,849 + HMPREF1058_RS02765 0.64 +2.1 99,976 + HMPREF1058_RS02765 0.75 +1.0 99,977 - HMPREF1058_RS02765 0.75 +2.2 100,032 + HMPREF1058_RS02765 0.79 +1.5 100,044 - HMPREF1058_RS02765 0.80 +1.5 100,197 + +0.8 100,228 + +1.4 100,245 - +0.8 100,313 - -0.1 100,313 - -1.4 100,370 + +0.4 100,388 + +1.8 100,423 + HMPREF1058_RS02760 0.14 +1.4 100,425 + HMPREF1058_RS02760 0.14 +2.9 100,465 - HMPREF1058_RS02760 0.19 -0.5 100,547 + HMPREF1058_RS02760 0.29 +1.3 100,547 + HMPREF1058_RS02760 0.29 +1.7 100,591 - HMPREF1058_RS02760 0.34 -1.4 100,593 - HMPREF1058_RS02760 0.34 +0.5 100,652 + HMPREF1058_RS02760 0.41 +0.6 100,710 + HMPREF1058_RS02760 0.48 +1.7 100,710 + HMPREF1058_RS02760 0.48 -0.4 100,711 - HMPREF1058_RS02760 0.48 -0.6 100,936 - HMPREF1058_RS02760 0.75 -0.2 100,937 + HMPREF1058_RS02760 0.75 +1.4 100,938 - HMPREF1058_RS02760 0.75 -0.5 100,991 - HMPREF1058_RS02760 0.81 +1.1 100,992 + HMPREF1058_RS02760 0.81 +1.4 101,124 - -0.5 101,127 - +0.3 101,197 + +0.9 101,251 + +0.4
Or see this region's nucleotide sequence