Experiment: L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS16415 and HMPREF1058_RS16410 are separated by 149 nucleotides HMPREF1058_RS16410 and HMPREF1058_RS16405 are separated by 178 nucleotides HMPREF1058_RS16405 and HMPREF1058_RS16400 are separated by 92 nucleotides
HMPREF1058_RS16415: HMPREF1058_RS16415 - metallophosphoesterase, at 676,749 to 677,912
_RS16415
HMPREF1058_RS16410: HMPREF1058_RS16410 - L-serine ammonia-lyase, at 678,062 to 679,270
_RS16410
HMPREF1058_RS16405: HMPREF1058_RS16405 - PepSY-like domain-containing protein, at 679,449 to 679,889
_RS16405
HMPREF1058_RS16400: HMPREF1058_RS16400 - DUF4738 domain-containing protein, at 679,982 to 680,551
_RS16400
Position (kb) 678
679
680 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 677.156 kb on + strand, within HMPREF1058_RS16415 at 677.156 kb on + strand, within HMPREF1058_RS16415 at 677.156 kb on + strand, within HMPREF1058_RS16415 at 677.157 kb on - strand, within HMPREF1058_RS16415 at 677.262 kb on + strand, within HMPREF1058_RS16415 at 677.302 kb on + strand, within HMPREF1058_RS16415 at 677.414 kb on - strand, within HMPREF1058_RS16415 at 677.487 kb on + strand, within HMPREF1058_RS16415 at 677.488 kb on - strand, within HMPREF1058_RS16415 at 677.491 kb on + strand, within HMPREF1058_RS16415 at 677.491 kb on + strand, within HMPREF1058_RS16415 at 677.491 kb on + strand at 677.491 kb on + strand, within HMPREF1058_RS16415 at 677.492 kb on - strand, within HMPREF1058_RS16415 at 677.492 kb on - strand, within HMPREF1058_RS16415 at 677.492 kb on - strand, within HMPREF1058_RS16415 at 677.570 kb on - strand, within HMPREF1058_RS16415 at 677.570 kb on - strand, within HMPREF1058_RS16415 at 677.800 kb on + strand at 677.894 kb on - strand at 677.894 kb on - strand at 677.894 kb on - strand at 677.906 kb on - strand at 677.919 kb on + strand at 677.968 kb on - strand at 678.038 kb on + strand at 678.038 kb on + strand at 678.041 kb on + strand at 678.041 kb on + strand at 678.041 kb on + strand at 678.042 kb on - strand at 678.042 kb on - strand at 678.082 kb on - strand at 678.084 kb on - strand at 678.118 kb on + strand at 678.248 kb on - strand, within HMPREF1058_RS16410 at 678.248 kb on - strand, within HMPREF1058_RS16410 at 678.438 kb on - strand, within HMPREF1058_RS16410 at 678.442 kb on + strand, within HMPREF1058_RS16410 at 678.442 kb on + strand, within HMPREF1058_RS16410 at 678.495 kb on - strand, within HMPREF1058_RS16410 at 678.552 kb on + strand, within HMPREF1058_RS16410 at 678.552 kb on + strand, within HMPREF1058_RS16410 at 678.644 kb on + strand, within HMPREF1058_RS16410 at 678.645 kb on - strand, within HMPREF1058_RS16410 at 678.650 kb on - strand, within HMPREF1058_RS16410 at 678.697 kb on - strand, within HMPREF1058_RS16410 at 678.798 kb on - strand, within HMPREF1058_RS16410 at 678.874 kb on + strand, within HMPREF1058_RS16410 at 678.874 kb on + strand, within HMPREF1058_RS16410 at 678.875 kb on - strand, within HMPREF1058_RS16410 at 678.875 kb on - strand, within HMPREF1058_RS16410 at 678.879 kb on + strand, within HMPREF1058_RS16410 at 678.880 kb on - strand, within HMPREF1058_RS16410 at 678.930 kb on + strand, within HMPREF1058_RS16410 at 679.002 kb on - strand, within HMPREF1058_RS16410 at 679.079 kb on - strand, within HMPREF1058_RS16410 at 679.079 kb on - strand, within HMPREF1058_RS16410 at 679.094 kb on + strand, within HMPREF1058_RS16410 at 679.215 kb on + strand at 679.216 kb on - strand at 679.217 kb on + strand at 679.217 kb on + strand at 679.220 kb on - strand at 679.309 kb on - strand at 679.309 kb on - strand at 679.337 kb on + strand at 679.338 kb on - strand at 679.356 kb on - strand at 679.395 kb on + strand at 679.407 kb on - strand at 679.421 kb on + strand at 679.422 kb on - strand at 679.492 kb on - strand at 679.535 kb on + strand, within HMPREF1058_RS16405 at 679.556 kb on + strand, within HMPREF1058_RS16405 at 679.556 kb on + strand, within HMPREF1058_RS16405 at 679.557 kb on - strand, within HMPREF1058_RS16405 at 679.567 kb on + strand, within HMPREF1058_RS16405 at 679.580 kb on + strand, within HMPREF1058_RS16405 at 679.582 kb on + strand, within HMPREF1058_RS16405 at 679.592 kb on + strand, within HMPREF1058_RS16405 at 679.638 kb on + strand, within HMPREF1058_RS16405 at 679.639 kb on - strand, within HMPREF1058_RS16405 at 679.740 kb on - strand, within HMPREF1058_RS16405 at 679.863 kb on - strand at 679.899 kb on + strand at 679.983 kb on + strand at 680.063 kb on - strand, within HMPREF1058_RS16400 at 680.085 kb on + strand, within HMPREF1058_RS16400 at 680.085 kb on + strand, within HMPREF1058_RS16400 at 680.086 kb on - strand, within HMPREF1058_RS16400 at 680.123 kb on + strand, within HMPREF1058_RS16400 at 680.180 kb on - strand, within HMPREF1058_RS16400 at 680.204 kb on + strand, within HMPREF1058_RS16400
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 677,156 + HMPREF1058_RS16415 0.35 +1.2 677,156 + HMPREF1058_RS16415 0.35 +0.2 677,156 + HMPREF1058_RS16415 0.35 -0.5 677,157 - HMPREF1058_RS16415 0.35 +1.2 677,262 + HMPREF1058_RS16415 0.44 -1.5 677,302 + HMPREF1058_RS16415 0.48 -0.3 677,414 - HMPREF1058_RS16415 0.57 +0.3 677,487 + HMPREF1058_RS16415 0.63 -1.7 677,488 - HMPREF1058_RS16415 0.63 +0.2 677,491 + HMPREF1058_RS16415 0.64 +0.4 677,491 + HMPREF1058_RS16415 0.64 -1.3 677,491 + +1.5 677,491 + HMPREF1058_RS16415 0.64 -1.1 677,492 - HMPREF1058_RS16415 0.64 -1.0 677,492 - HMPREF1058_RS16415 0.64 +1.1 677,492 - HMPREF1058_RS16415 0.64 -0.6 677,570 - HMPREF1058_RS16415 0.71 +1.0 677,570 - HMPREF1058_RS16415 0.71 -0.6 677,800 + -1.6 677,894 - -1.7 677,894 - -1.2 677,894 - -0.3 677,906 - -0.0 677,919 + -0.3 677,968 - +3.2 678,038 + +0.8 678,038 + -1.6 678,041 + +0.7 678,041 + +0.3 678,041 + +0.7 678,042 - -3.9 678,042 - -1.7 678,082 - -2.8 678,084 - -1.1 678,118 + -1.9 678,248 - HMPREF1058_RS16410 0.15 -2.7 678,248 - HMPREF1058_RS16410 0.15 -0.6 678,438 - HMPREF1058_RS16410 0.31 +0.7 678,442 + HMPREF1058_RS16410 0.31 -2.5 678,442 + HMPREF1058_RS16410 0.31 -1.5 678,495 - HMPREF1058_RS16410 0.36 -2.7 678,552 + HMPREF1058_RS16410 0.41 -1.3 678,552 + HMPREF1058_RS16410 0.41 -3.3 678,644 + HMPREF1058_RS16410 0.48 -2.2 678,645 - HMPREF1058_RS16410 0.48 -0.6 678,650 - HMPREF1058_RS16410 0.49 +0.2 678,697 - HMPREF1058_RS16410 0.53 -0.1 678,798 - HMPREF1058_RS16410 0.61 -4.8 678,874 + HMPREF1058_RS16410 0.67 -2.7 678,874 + HMPREF1058_RS16410 0.67 -2.4 678,875 - HMPREF1058_RS16410 0.67 -1.9 678,875 - HMPREF1058_RS16410 0.67 -1.5 678,879 + HMPREF1058_RS16410 0.68 -0.6 678,880 - HMPREF1058_RS16410 0.68 -2.8 678,930 + HMPREF1058_RS16410 0.72 -2.2 679,002 - HMPREF1058_RS16410 0.78 -2.1 679,079 - HMPREF1058_RS16410 0.84 -2.1 679,079 - HMPREF1058_RS16410 0.84 -0.1 679,094 + HMPREF1058_RS16410 0.85 -3.0 679,215 + -2.6 679,216 - -0.3 679,217 + -1.5 679,217 + -2.5 679,220 - -2.5 679,309 - -0.6 679,309 - -0.3 679,337 + +0.2 679,338 - -0.2 679,356 - -0.2 679,395 + -1.1 679,407 - +1.2 679,421 + +1.4 679,422 - -0.6 679,492 - +0.8 679,535 + HMPREF1058_RS16405 0.20 +0.2 679,556 + HMPREF1058_RS16405 0.24 +1.0 679,556 + HMPREF1058_RS16405 0.24 -1.7 679,557 - HMPREF1058_RS16405 0.24 +0.6 679,567 + HMPREF1058_RS16405 0.27 -0.3 679,580 + HMPREF1058_RS16405 0.30 +0.0 679,582 + HMPREF1058_RS16405 0.30 -2.9 679,592 + HMPREF1058_RS16405 0.32 -0.6 679,638 + HMPREF1058_RS16405 0.43 -0.8 679,639 - HMPREF1058_RS16405 0.43 -0.6 679,740 - HMPREF1058_RS16405 0.66 -1.0 679,863 - +2.5 679,899 + -1.4 679,983 + +1.0 680,063 - HMPREF1058_RS16400 0.14 +2.7 680,085 + HMPREF1058_RS16400 0.18 +2.4 680,085 + HMPREF1058_RS16400 0.18 +0.8 680,086 - HMPREF1058_RS16400 0.18 +2.4 680,123 + HMPREF1058_RS16400 0.25 +1.3 680,180 - HMPREF1058_RS16400 0.35 +2.2 680,204 + HMPREF1058_RS16400 0.39 +1.1
Or see this region's nucleotide sequence