Experiment: L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS18115 and HMPREF1058_RS18110 are separated by 101 nucleotides HMPREF1058_RS18110 and HMPREF1058_RS18105 are separated by 3 nucleotides HMPREF1058_RS18105 and HMPREF1058_RS18100 overlap by 4 nucleotides
HMPREF1058_RS18115: HMPREF1058_RS18115 - pyruvate formate lyase-activating protein, at 256,435 to 257,163
_RS18115
HMPREF1058_RS18110: HMPREF1058_RS18110 - LUD domain-containing protein, at 257,265 to 257,852
_RS18110
HMPREF1058_RS18105: HMPREF1058_RS18105 - lactate utilization protein, at 257,856 to 259,223
_RS18105
HMPREF1058_RS18100: HMPREF1058_RS18100 - (Fe-S)-binding protein, at 259,220 to 259,960
_RS18100
Position (kb) 257
258
259
260 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 256.871 kb on + strand, within HMPREF1058_RS18115 at 256.871 kb on + strand, within HMPREF1058_RS18115 at 256.872 kb on - strand, within HMPREF1058_RS18115 at 256.872 kb on - strand, within HMPREF1058_RS18115 at 256.876 kb on - strand, within HMPREF1058_RS18115 at 256.926 kb on - strand, within HMPREF1058_RS18115 at 256.928 kb on + strand, within HMPREF1058_RS18115 at 256.940 kb on - strand, within HMPREF1058_RS18115 at 256.942 kb on - strand, within HMPREF1058_RS18115 at 256.962 kb on - strand, within HMPREF1058_RS18115 at 257.009 kb on + strand, within HMPREF1058_RS18115 at 257.015 kb on + strand, within HMPREF1058_RS18115 at 257.015 kb on + strand, within HMPREF1058_RS18115 at 257.015 kb on + strand, within HMPREF1058_RS18115 at 257.018 kb on + strand, within HMPREF1058_RS18115 at 257.019 kb on - strand, within HMPREF1058_RS18115 at 257.019 kb on - strand, within HMPREF1058_RS18115 at 257.028 kb on + strand, within HMPREF1058_RS18115 at 257.028 kb on + strand, within HMPREF1058_RS18115 at 257.029 kb on - strand, within HMPREF1058_RS18115 at 257.047 kb on + strand, within HMPREF1058_RS18115 at 257.100 kb on - strand at 257.151 kb on - strand at 257.161 kb on + strand at 257.195 kb on - strand at 257.244 kb on + strand at 257.248 kb on + strand at 257.249 kb on - strand at 257.262 kb on + strand at 257.262 kb on + strand at 257.262 kb on + strand at 257.263 kb on - strand at 257.266 kb on + strand at 257.266 kb on + strand at 257.267 kb on - strand at 257.269 kb on - strand at 257.381 kb on + strand, within HMPREF1058_RS18110 at 257.386 kb on - strand, within HMPREF1058_RS18110 at 257.395 kb on + strand, within HMPREF1058_RS18110 at 257.408 kb on + strand, within HMPREF1058_RS18110 at 257.424 kb on - strand, within HMPREF1058_RS18110 at 257.429 kb on + strand, within HMPREF1058_RS18110 at 257.460 kb on - strand, within HMPREF1058_RS18110 at 257.481 kb on + strand, within HMPREF1058_RS18110 at 257.535 kb on + strand, within HMPREF1058_RS18110 at 257.536 kb on - strand, within HMPREF1058_RS18110 at 257.606 kb on + strand, within HMPREF1058_RS18110 at 257.606 kb on + strand, within HMPREF1058_RS18110 at 257.607 kb on - strand, within HMPREF1058_RS18110 at 257.607 kb on - strand, within HMPREF1058_RS18110 at 257.607 kb on - strand, within HMPREF1058_RS18110 at 257.709 kb on + strand, within HMPREF1058_RS18110 at 257.729 kb on - strand, within HMPREF1058_RS18110 at 257.753 kb on - strand, within HMPREF1058_RS18110 at 257.765 kb on + strand, within HMPREF1058_RS18110 at 257.798 kb on - strand at 257.802 kb on + strand at 257.832 kb on + strand at 257.832 kb on + strand at 257.833 kb on - strand at 257.833 kb on - strand at 257.834 kb on + strand at 257.835 kb on - strand at 257.950 kb on + strand at 257.951 kb on - strand at 258.003 kb on - strand, within HMPREF1058_RS18105 at 258.142 kb on - strand, within HMPREF1058_RS18105 at 258.191 kb on - strand, within HMPREF1058_RS18105 at 258.191 kb on - strand, within HMPREF1058_RS18105 at 258.253 kb on + strand, within HMPREF1058_RS18105 at 258.270 kb on - strand, within HMPREF1058_RS18105 at 258.270 kb on - strand, within HMPREF1058_RS18105 at 258.276 kb on - strand, within HMPREF1058_RS18105 at 258.280 kb on - strand, within HMPREF1058_RS18105 at 258.291 kb on - strand, within HMPREF1058_RS18105 at 258.291 kb on - strand, within HMPREF1058_RS18105 at 258.291 kb on - strand, within HMPREF1058_RS18105 at 258.291 kb on - strand, within HMPREF1058_RS18105 at 258.291 kb on - strand, within HMPREF1058_RS18105 at 258.296 kb on + strand, within HMPREF1058_RS18105 at 258.332 kb on + strand, within HMPREF1058_RS18105 at 258.356 kb on + strand, within HMPREF1058_RS18105 at 258.359 kb on + strand, within HMPREF1058_RS18105 at 258.360 kb on - strand, within HMPREF1058_RS18105 at 258.377 kb on + strand, within HMPREF1058_RS18105 at 258.380 kb on + strand, within HMPREF1058_RS18105 at 258.381 kb on - strand, within HMPREF1058_RS18105 at 258.425 kb on + strand, within HMPREF1058_RS18105 at 258.425 kb on + strand, within HMPREF1058_RS18105 at 258.426 kb on - strand, within HMPREF1058_RS18105 at 258.426 kb on - strand, within HMPREF1058_RS18105 at 258.427 kb on + strand, within HMPREF1058_RS18105 at 258.427 kb on + strand, within HMPREF1058_RS18105 at 258.427 kb on + strand, within HMPREF1058_RS18105 at 258.432 kb on - strand, within HMPREF1058_RS18105 at 258.540 kb on - strand, within HMPREF1058_RS18105 at 258.578 kb on + strand, within HMPREF1058_RS18105 at 258.579 kb on - strand, within HMPREF1058_RS18105 at 258.582 kb on + strand, within HMPREF1058_RS18105 at 258.683 kb on + strand, within HMPREF1058_RS18105 at 258.684 kb on - strand, within HMPREF1058_RS18105 at 258.688 kb on + strand, within HMPREF1058_RS18105 at 258.692 kb on + strand, within HMPREF1058_RS18105 at 258.693 kb on - strand, within HMPREF1058_RS18105 at 258.745 kb on - strand, within HMPREF1058_RS18105 at 258.816 kb on - strand, within HMPREF1058_RS18105 at 258.816 kb on - strand, within HMPREF1058_RS18105 at 258.872 kb on + strand, within HMPREF1058_RS18105 at 258.956 kb on + strand, within HMPREF1058_RS18105 at 259.052 kb on + strand, within HMPREF1058_RS18105 at 259.053 kb on - strand, within HMPREF1058_RS18105 at 259.056 kb on - strand, within HMPREF1058_RS18105 at 259.056 kb on - strand, within HMPREF1058_RS18105 at 259.060 kb on - strand, within HMPREF1058_RS18105 at 259.060 kb on - strand, within HMPREF1058_RS18105 at 259.067 kb on + strand, within HMPREF1058_RS18105 at 259.079 kb on + strand, within HMPREF1058_RS18105 at 259.112 kb on + strand at 259.224 kb on - strand at 259.224 kb on - strand at 259.332 kb on + strand, within HMPREF1058_RS18100 at 259.352 kb on - strand, within HMPREF1058_RS18100 at 259.473 kb on - strand, within HMPREF1058_RS18100 at 259.475 kb on + strand, within HMPREF1058_RS18100 at 259.475 kb on + strand, within HMPREF1058_RS18100 at 259.583 kb on - strand, within HMPREF1058_RS18100 at 259.643 kb on - strand, within HMPREF1058_RS18100 at 259.647 kb on + strand, within HMPREF1058_RS18100 at 259.647 kb on + strand, within HMPREF1058_RS18100 at 259.648 kb on - strand, within HMPREF1058_RS18100 at 259.648 kb on - strand, within HMPREF1058_RS18100 at 259.650 kb on - strand, within HMPREF1058_RS18100 at 259.655 kb on - strand, within HMPREF1058_RS18100 at 259.701 kb on + strand, within HMPREF1058_RS18100 at 259.702 kb on - strand, within HMPREF1058_RS18100 at 259.702 kb on - strand, within HMPREF1058_RS18100 at 259.747 kb on + strand, within HMPREF1058_RS18100 at 259.747 kb on + strand, within HMPREF1058_RS18100 at 259.748 kb on - strand, within HMPREF1058_RS18100 at 259.748 kb on - strand, within HMPREF1058_RS18100 at 259.932 kb on + strand at 259.952 kb on + strand at 259.953 kb on - strand at 259.968 kb on - strand at 259.972 kb on - strand at 259.983 kb on + strand at 260.069 kb on + strand at 260.072 kb on + strand at 260.090 kb on + strand at 260.091 kb on - strand at 260.093 kb on + strand at 260.142 kb on + strand at 260.146 kb on - strand at 260.182 kb on - strand at 260.186 kb on + strand at 260.187 kb on - strand at 260.187 kb on - strand at 260.187 kb on - strand at 260.200 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 256,871 + HMPREF1058_RS18115 0.60 +0.3 256,871 + HMPREF1058_RS18115 0.60 +0.5 256,872 - HMPREF1058_RS18115 0.60 -0.1 256,872 - HMPREF1058_RS18115 0.60 -0.9 256,876 - HMPREF1058_RS18115 0.60 -0.5 256,926 - HMPREF1058_RS18115 0.67 -1.7 256,928 + HMPREF1058_RS18115 0.68 +0.2 256,940 - HMPREF1058_RS18115 0.69 +0.2 256,942 - HMPREF1058_RS18115 0.70 -0.5 256,962 - HMPREF1058_RS18115 0.72 -0.9 257,009 + HMPREF1058_RS18115 0.79 +2.2 257,015 + HMPREF1058_RS18115 0.80 -0.6 257,015 + HMPREF1058_RS18115 0.80 +0.8 257,015 + HMPREF1058_RS18115 0.80 -0.8 257,018 + HMPREF1058_RS18115 0.80 -0.2 257,019 - HMPREF1058_RS18115 0.80 -1.9 257,019 - HMPREF1058_RS18115 0.80 -0.9 257,028 + HMPREF1058_RS18115 0.81 -2.2 257,028 + HMPREF1058_RS18115 0.81 -0.9 257,029 - HMPREF1058_RS18115 0.81 -0.9 257,047 + HMPREF1058_RS18115 0.84 -0.7 257,100 - -0.2 257,151 - +2.1 257,161 + -1.6 257,195 - -2.3 257,244 + -0.4 257,248 + +0.5 257,249 - -0.4 257,262 + -3.1 257,262 + +3.1 257,262 + -0.3 257,263 - -0.6 257,266 + +0.6 257,266 + +0.5 257,267 - +1.4 257,269 - -0.2 257,381 + HMPREF1058_RS18110 0.20 -0.8 257,386 - HMPREF1058_RS18110 0.21 +0.7 257,395 + HMPREF1058_RS18110 0.22 -0.8 257,408 + HMPREF1058_RS18110 0.24 -1.6 257,424 - HMPREF1058_RS18110 0.27 -0.6 257,429 + HMPREF1058_RS18110 0.28 -0.4 257,460 - HMPREF1058_RS18110 0.33 -1.4 257,481 + HMPREF1058_RS18110 0.37 -2.8 257,535 + HMPREF1058_RS18110 0.46 +0.5 257,536 - HMPREF1058_RS18110 0.46 -2.1 257,606 + HMPREF1058_RS18110 0.58 -2.5 257,606 + HMPREF1058_RS18110 0.58 -0.1 257,607 - HMPREF1058_RS18110 0.58 +1.0 257,607 - HMPREF1058_RS18110 0.58 -0.7 257,607 - HMPREF1058_RS18110 0.58 -0.5 257,709 + HMPREF1058_RS18110 0.76 +0.8 257,729 - HMPREF1058_RS18110 0.79 -1.7 257,753 - HMPREF1058_RS18110 0.83 +1.4 257,765 + HMPREF1058_RS18110 0.85 +0.1 257,798 - +2.1 257,802 + -1.2 257,832 + -2.6 257,832 + -0.7 257,833 - -3.1 257,833 - -2.0 257,834 + -2.2 257,835 - -0.9 257,950 + +1.1 257,951 - +1.1 258,003 - HMPREF1058_RS18105 0.11 +0.3 258,142 - HMPREF1058_RS18105 0.21 -2.5 258,191 - HMPREF1058_RS18105 0.24 -0.7 258,191 - HMPREF1058_RS18105 0.24 -2.6 258,253 + HMPREF1058_RS18105 0.29 -0.5 258,270 - HMPREF1058_RS18105 0.30 +0.6 258,270 - HMPREF1058_RS18105 0.30 -0.9 258,276 - HMPREF1058_RS18105 0.31 +0.1 258,280 - HMPREF1058_RS18105 0.31 -0.7 258,291 - HMPREF1058_RS18105 0.32 +0.5 258,291 - HMPREF1058_RS18105 0.32 -1.3 258,291 - HMPREF1058_RS18105 0.32 -2.3 258,291 - HMPREF1058_RS18105 0.32 -1.8 258,291 - HMPREF1058_RS18105 0.32 -0.9 258,296 + HMPREF1058_RS18105 0.32 -0.3 258,332 + HMPREF1058_RS18105 0.35 +0.2 258,356 + HMPREF1058_RS18105 0.37 +0.1 258,359 + HMPREF1058_RS18105 0.37 -2.2 258,360 - HMPREF1058_RS18105 0.37 +2.1 258,377 + HMPREF1058_RS18105 0.38 -0.8 258,380 + HMPREF1058_RS18105 0.38 +1.7 258,381 - HMPREF1058_RS18105 0.38 +3.0 258,425 + HMPREF1058_RS18105 0.42 +0.1 258,425 + HMPREF1058_RS18105 0.42 -0.2 258,426 - HMPREF1058_RS18105 0.42 -1.6 258,426 - HMPREF1058_RS18105 0.42 -1.0 258,427 + HMPREF1058_RS18105 0.42 +0.8 258,427 + HMPREF1058_RS18105 0.42 -0.7 258,427 + HMPREF1058_RS18105 0.42 -2.2 258,432 - HMPREF1058_RS18105 0.42 -1.0 258,540 - HMPREF1058_RS18105 0.50 -1.5 258,578 + HMPREF1058_RS18105 0.53 -2.4 258,579 - HMPREF1058_RS18105 0.53 -0.6 258,582 + HMPREF1058_RS18105 0.53 -0.2 258,683 + HMPREF1058_RS18105 0.60 -3.1 258,684 - HMPREF1058_RS18105 0.61 -1.8 258,688 + HMPREF1058_RS18105 0.61 +0.5 258,692 + HMPREF1058_RS18105 0.61 -0.5 258,693 - HMPREF1058_RS18105 0.61 -1.9 258,745 - HMPREF1058_RS18105 0.65 -2.6 258,816 - HMPREF1058_RS18105 0.70 -0.0 258,816 - HMPREF1058_RS18105 0.70 +0.5 258,872 + HMPREF1058_RS18105 0.74 -0.3 258,956 + HMPREF1058_RS18105 0.80 -0.9 259,052 + HMPREF1058_RS18105 0.87 +1.3 259,053 - HMPREF1058_RS18105 0.88 -2.2 259,056 - HMPREF1058_RS18105 0.88 -0.9 259,056 - HMPREF1058_RS18105 0.88 -0.5 259,060 - HMPREF1058_RS18105 0.88 -0.3 259,060 - HMPREF1058_RS18105 0.88 +0.2 259,067 + HMPREF1058_RS18105 0.89 -0.6 259,079 + HMPREF1058_RS18105 0.89 -1.6 259,112 + -0.4 259,224 - -1.2 259,224 - -0.3 259,332 + HMPREF1058_RS18100 0.15 +2.7 259,352 - HMPREF1058_RS18100 0.18 +0.6 259,473 - HMPREF1058_RS18100 0.34 -0.5 259,475 + HMPREF1058_RS18100 0.34 +0.7 259,475 + HMPREF1058_RS18100 0.34 -0.3 259,583 - HMPREF1058_RS18100 0.49 -0.9 259,643 - HMPREF1058_RS18100 0.57 +0.6 259,647 + HMPREF1058_RS18100 0.58 -0.2 259,647 + HMPREF1058_RS18100 0.58 -0.2 259,648 - HMPREF1058_RS18100 0.58 -1.4 259,648 - HMPREF1058_RS18100 0.58 -0.2 259,650 - HMPREF1058_RS18100 0.58 -1.2 259,655 - HMPREF1058_RS18100 0.59 -1.4 259,701 + HMPREF1058_RS18100 0.65 -1.9 259,702 - HMPREF1058_RS18100 0.65 +0.4 259,702 - HMPREF1058_RS18100 0.65 -1.7 259,747 + HMPREF1058_RS18100 0.71 -3.7 259,747 + HMPREF1058_RS18100 0.71 -0.6 259,748 - HMPREF1058_RS18100 0.71 -1.3 259,748 - HMPREF1058_RS18100 0.71 +0.5 259,932 + +0.3 259,952 + -0.8 259,953 - -1.8 259,968 - +0.6 259,972 - +3.1 259,983 + +0.5 260,069 + -0.5 260,072 + +2.7 260,090 + +0.7 260,091 - -1.8 260,093 + +0.7 260,142 + -2.5 260,146 - +0.3 260,182 - -0.1 260,186 + +0.2 260,187 - +0.8 260,187 - +0.2 260,187 - +0.9 260,200 - +0.4
Or see this region's nucleotide sequence