Experiment: L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17640 and HMPREF1058_RS17635 are separated by 265 nucleotides HMPREF1058_RS17635 and HMPREF1058_RS17630 are separated by 267 nucleotides HMPREF1058_RS17630 and HMPREF1058_RS17625 overlap by 4 nucleotides
HMPREF1058_RS17640: HMPREF1058_RS17640 - L-lactate permease, at 380,708 to 382,207
_RS17640
HMPREF1058_RS17635: HMPREF1058_RS17635 - O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase, at 382,473 to 383,759
_RS17635
HMPREF1058_RS17630: HMPREF1058_RS17630 - DUF4625 domain-containing protein, at 384,027 to 384,521
_RS17630
HMPREF1058_RS17625: HMPREF1058_RS17625 - TonB-dependent receptor, at 384,518 to 386,677
_RS17625
Position (kb) 382
383
384 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 381.485 kb on - strand, within HMPREF1058_RS17640 at 381.494 kb on - strand, within HMPREF1058_RS17640 at 381.517 kb on + strand, within HMPREF1058_RS17640 at 381.518 kb on - strand, within HMPREF1058_RS17640 at 381.587 kb on - strand, within HMPREF1058_RS17640 at 381.587 kb on - strand, within HMPREF1058_RS17640 at 381.626 kb on + strand, within HMPREF1058_RS17640 at 381.830 kb on + strand, within HMPREF1058_RS17640 at 381.830 kb on + strand, within HMPREF1058_RS17640 at 381.831 kb on - strand, within HMPREF1058_RS17640 at 381.833 kb on - strand, within HMPREF1058_RS17640 at 381.899 kb on + strand, within HMPREF1058_RS17640 at 382.041 kb on + strand, within HMPREF1058_RS17640 at 382.105 kb on + strand at 382.126 kb on + strand at 382.172 kb on + strand at 382.173 kb on - strand at 382.189 kb on + strand at 382.220 kb on + strand at 382.257 kb on - strand at 382.315 kb on - strand at 382.374 kb on + strand at 382.374 kb on + strand at 382.375 kb on - strand at 382.377 kb on - strand at 382.419 kb on + strand at 382.420 kb on - strand at 382.421 kb on + strand at 382.437 kb on + strand at 382.443 kb on + strand at 382.444 kb on - strand at 382.455 kb on + strand at 382.455 kb on + strand at 382.455 kb on + strand at 382.455 kb on + strand at 382.460 kb on - strand at 382.460 kb on - strand at 382.462 kb on - strand at 382.465 kb on - strand at 382.465 kb on - strand at 382.465 kb on - strand at 382.466 kb on + strand at 382.466 kb on + strand at 382.518 kb on + strand at 382.518 kb on + strand at 382.519 kb on - strand at 382.534 kb on - strand at 382.563 kb on - strand at 382.563 kb on - strand at 382.753 kb on + strand, within HMPREF1058_RS17635 at 382.762 kb on + strand, within HMPREF1058_RS17635 at 382.762 kb on + strand, within HMPREF1058_RS17635 at 382.931 kb on + strand, within HMPREF1058_RS17635 at 382.953 kb on + strand, within HMPREF1058_RS17635 at 383.146 kb on - strand, within HMPREF1058_RS17635 at 383.146 kb on - strand, within HMPREF1058_RS17635 at 383.153 kb on + strand, within HMPREF1058_RS17635 at 383.153 kb on + strand, within HMPREF1058_RS17635 at 383.211 kb on - strand, within HMPREF1058_RS17635 at 383.220 kb on + strand, within HMPREF1058_RS17635 at 383.239 kb on - strand, within HMPREF1058_RS17635 at 383.256 kb on + strand, within HMPREF1058_RS17635 at 383.256 kb on + strand, within HMPREF1058_RS17635 at 383.257 kb on - strand, within HMPREF1058_RS17635 at 383.262 kb on + strand, within HMPREF1058_RS17635 at 383.263 kb on - strand, within HMPREF1058_RS17635 at 383.324 kb on + strand, within HMPREF1058_RS17635 at 383.366 kb on + strand, within HMPREF1058_RS17635 at 383.367 kb on - strand, within HMPREF1058_RS17635 at 383.380 kb on + strand, within HMPREF1058_RS17635 at 383.410 kb on + strand, within HMPREF1058_RS17635 at 383.410 kb on + strand, within HMPREF1058_RS17635 at 383.411 kb on - strand, within HMPREF1058_RS17635 at 383.411 kb on - strand, within HMPREF1058_RS17635 at 383.411 kb on - strand, within HMPREF1058_RS17635 at 383.414 kb on + strand, within HMPREF1058_RS17635 at 383.427 kb on - strand, within HMPREF1058_RS17635 at 383.439 kb on + strand, within HMPREF1058_RS17635 at 383.650 kb on + strand at 383.658 kb on - strand at 383.669 kb on + strand at 383.715 kb on + strand at 383.715 kb on + strand at 383.780 kb on + strand at 383.780 kb on + strand at 383.781 kb on - strand at 383.782 kb on + strand at 383.796 kb on + strand at 383.825 kb on - strand at 383.839 kb on - strand at 383.998 kb on - strand at 383.998 kb on - strand at 384.084 kb on - strand, within HMPREF1058_RS17630 at 384.189 kb on + strand, within HMPREF1058_RS17630 at 384.197 kb on + strand, within HMPREF1058_RS17630 at 384.220 kb on + strand, within HMPREF1058_RS17630 at 384.221 kb on - strand, within HMPREF1058_RS17630 at 384.222 kb on + strand, within HMPREF1058_RS17630 at 384.405 kb on + strand, within HMPREF1058_RS17630 at 384.429 kb on + strand, within HMPREF1058_RS17630 at 384.429 kb on + strand, within HMPREF1058_RS17630 at 384.430 kb on - strand, within HMPREF1058_RS17630 at 384.430 kb on - strand, within HMPREF1058_RS17630 at 384.430 kb on - strand, within HMPREF1058_RS17630 at 384.478 kb on - strand at 384.480 kb on - strand at 384.496 kb on - strand at 384.535 kb on + strand at 384.598 kb on - strand at 384.637 kb on + strand at 384.637 kb on + strand at 384.676 kb on + strand at 384.680 kb on + strand at 384.681 kb on - strand at 384.681 kb on - strand at 384.682 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 381,485 - HMPREF1058_RS17640 0.52 -1.2 381,494 - HMPREF1058_RS17640 0.52 -1.1 381,517 + HMPREF1058_RS17640 0.54 +0.1 381,518 - HMPREF1058_RS17640 0.54 -0.5 381,587 - HMPREF1058_RS17640 0.59 -0.5 381,587 - HMPREF1058_RS17640 0.59 -2.2 381,626 + HMPREF1058_RS17640 0.61 -1.8 381,830 + HMPREF1058_RS17640 0.75 +0.1 381,830 + HMPREF1058_RS17640 0.75 +0.9 381,831 - HMPREF1058_RS17640 0.75 +0.2 381,833 - HMPREF1058_RS17640 0.75 +0.7 381,899 + HMPREF1058_RS17640 0.79 +0.5 382,041 + HMPREF1058_RS17640 0.89 -2.3 382,105 + +0.5 382,126 + -1.2 382,172 + +1.5 382,173 - -0.2 382,189 + -1.4 382,220 + +1.5 382,257 - -0.9 382,315 - -0.7 382,374 + +0.5 382,374 + +0.5 382,375 - +1.2 382,377 - -1.0 382,419 + +0.6 382,420 - -0.5 382,421 + -0.2 382,437 + +1.1 382,443 + -0.4 382,444 - -0.1 382,455 + +1.3 382,455 + +2.1 382,455 + -0.6 382,455 + -0.1 382,460 - -1.6 382,460 - -2.2 382,462 - -1.1 382,465 - -0.9 382,465 - +2.1 382,465 - -0.7 382,466 + -0.9 382,466 + -0.7 382,518 + -2.2 382,518 + -0.9 382,519 - -3.5 382,534 - -1.8 382,563 - -2.4 382,563 - -1.9 382,753 + HMPREF1058_RS17635 0.22 +0.8 382,762 + HMPREF1058_RS17635 0.22 -1.2 382,762 + HMPREF1058_RS17635 0.22 -2.0 382,931 + HMPREF1058_RS17635 0.36 -1.2 382,953 + HMPREF1058_RS17635 0.37 -3.5 383,146 - HMPREF1058_RS17635 0.52 -1.2 383,146 - HMPREF1058_RS17635 0.52 -4.4 383,153 + HMPREF1058_RS17635 0.53 -0.2 383,153 + HMPREF1058_RS17635 0.53 -0.9 383,211 - HMPREF1058_RS17635 0.57 -0.5 383,220 + HMPREF1058_RS17635 0.58 -3.0 383,239 - HMPREF1058_RS17635 0.60 +2.1 383,256 + HMPREF1058_RS17635 0.61 -0.2 383,256 + HMPREF1058_RS17635 0.61 -2.7 383,257 - HMPREF1058_RS17635 0.61 +0.5 383,262 + HMPREF1058_RS17635 0.61 -1.6 383,263 - HMPREF1058_RS17635 0.61 -1.5 383,324 + HMPREF1058_RS17635 0.66 +0.1 383,366 + HMPREF1058_RS17635 0.69 -2.6 383,367 - HMPREF1058_RS17635 0.69 -1.1 383,380 + HMPREF1058_RS17635 0.70 -4.7 383,410 + HMPREF1058_RS17635 0.73 -1.0 383,410 + HMPREF1058_RS17635 0.73 -0.7 383,411 - HMPREF1058_RS17635 0.73 -3.0 383,411 - HMPREF1058_RS17635 0.73 -1.9 383,411 - HMPREF1058_RS17635 0.73 -2.7 383,414 + HMPREF1058_RS17635 0.73 -0.9 383,427 - HMPREF1058_RS17635 0.74 -1.9 383,439 + HMPREF1058_RS17635 0.75 -3.4 383,650 + -3.4 383,658 - -0.5 383,669 + -1.9 383,715 + -3.6 383,715 + -1.6 383,780 + +0.1 383,780 + +0.5 383,781 - -1.2 383,782 + -2.3 383,796 + -3.4 383,825 - +1.1 383,839 - -1.9 383,998 - -0.8 383,998 - +1.6 384,084 - HMPREF1058_RS17630 0.12 -0.8 384,189 + HMPREF1058_RS17630 0.33 -1.0 384,197 + HMPREF1058_RS17630 0.34 +0.2 384,220 + HMPREF1058_RS17630 0.39 -0.7 384,221 - HMPREF1058_RS17630 0.39 +0.3 384,222 + HMPREF1058_RS17630 0.39 -1.6 384,405 + HMPREF1058_RS17630 0.76 +2.0 384,429 + HMPREF1058_RS17630 0.81 +0.5 384,429 + HMPREF1058_RS17630 0.81 +1.7 384,430 - HMPREF1058_RS17630 0.81 -0.1 384,430 - HMPREF1058_RS17630 0.81 +1.1 384,430 - HMPREF1058_RS17630 0.81 -0.2 384,478 - -0.7 384,480 - -0.2 384,496 - -0.1 384,535 + +1.7 384,598 - -0.2 384,637 + +1.2 384,637 + -2.1 384,676 + +1.1 384,680 + -0.5 384,681 - -0.8 384,681 - -1.7 384,682 + +0.5
Or see this region's nucleotide sequence