Experiment: L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS17600 and HMPREF1058_RS17595 are separated by 191 nucleotides HMPREF1058_RS17595 and HMPREF1058_RS17590 are separated by 24 nucleotides HMPREF1058_RS17590 and HMPREF1058_RS17585 are separated by 474 nucleotides HMPREF1058_RS17585 and HMPREF1058_RS17580 are separated by 166 nucleotides
HMPREF1058_RS17600: HMPREF1058_RS17600 - hypothetical protein, at 389,721 to 390,146
_RS17600
HMPREF1058_RS17595: HMPREF1058_RS17595 - Lrp/AsnC family transcriptional regulator, at 390,338 to 390,808
_RS17595
HMPREF1058_RS17590: HMPREF1058_RS17590 - magnesium/cobalt transporter CorA, at 390,833 to 391,873
_RS17590
HMPREF1058_RS17585: HMPREF1058_RS17585 - helix-turn-helix transcriptional regulator, at 392,348 to 392,701
_RS17585
HMPREF1058_RS17580: HMPREF1058_RS17580 - dihydrofolate reductase, at 392,868 to 393,365
_RS17580
Position (kb) 390
391
392 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3
4 at 389.919 kb on - strand, within HMPREF1058_RS17600 at 389.945 kb on - strand, within HMPREF1058_RS17600 at 389.968 kb on - strand, within HMPREF1058_RS17600 at 389.993 kb on + strand, within HMPREF1058_RS17600 at 389.994 kb on - strand, within HMPREF1058_RS17600 at 390.008 kb on + strand, within HMPREF1058_RS17600 at 390.106 kb on - strand at 390.106 kb on - strand at 390.119 kb on + strand at 390.120 kb on - strand at 390.122 kb on - strand at 390.130 kb on + strand at 390.131 kb on - strand at 390.131 kb on - strand at 390.132 kb on + strand at 390.132 kb on + strand at 390.132 kb on + strand at 390.135 kb on - strand at 390.135 kb on - strand at 390.135 kb on - strand at 390.137 kb on - strand at 390.146 kb on + strand at 390.228 kb on + strand at 390.228 kb on + strand at 390.239 kb on + strand at 390.239 kb on + strand at 390.240 kb on - strand at 390.267 kb on + strand at 390.283 kb on + strand at 390.284 kb on - strand at 390.284 kb on - strand at 390.308 kb on - strand at 390.333 kb on - strand at 390.343 kb on + strand at 390.382 kb on - strand at 390.386 kb on - strand, within HMPREF1058_RS17595 at 390.386 kb on - strand, within HMPREF1058_RS17595 at 390.491 kb on + strand, within HMPREF1058_RS17595 at 390.491 kb on + strand, within HMPREF1058_RS17595 at 390.492 kb on - strand, within HMPREF1058_RS17595 at 390.567 kb on + strand, within HMPREF1058_RS17595 at 390.609 kb on + strand, within HMPREF1058_RS17595 at 390.630 kb on + strand, within HMPREF1058_RS17595 at 390.641 kb on + strand, within HMPREF1058_RS17595 at 390.642 kb on - strand, within HMPREF1058_RS17595 at 390.642 kb on - strand, within HMPREF1058_RS17595 at 390.666 kb on - strand, within HMPREF1058_RS17595 at 390.678 kb on + strand, within HMPREF1058_RS17595 at 390.698 kb on + strand, within HMPREF1058_RS17595 at 390.699 kb on - strand, within HMPREF1058_RS17595 at 390.806 kb on + strand at 390.811 kb on - strand at 390.853 kb on + strand at 390.864 kb on + strand at 390.865 kb on - strand at 390.869 kb on - strand at 390.869 kb on - strand at 390.882 kb on - strand at 390.905 kb on + strand at 390.906 kb on - strand at 390.906 kb on - strand at 390.909 kb on + strand at 390.910 kb on - strand at 390.989 kb on + strand, within HMPREF1058_RS17590 at 390.990 kb on - strand, within HMPREF1058_RS17590 at 390.992 kb on - strand, within HMPREF1058_RS17590 at 390.995 kb on - strand, within HMPREF1058_RS17590 at 391.053 kb on + strand, within HMPREF1058_RS17590 at 391.053 kb on + strand, within HMPREF1058_RS17590 at 391.054 kb on - strand, within HMPREF1058_RS17590 at 391.061 kb on + strand, within HMPREF1058_RS17590 at 391.095 kb on - strand, within HMPREF1058_RS17590 at 391.220 kb on - strand, within HMPREF1058_RS17590 at 391.354 kb on + strand, within HMPREF1058_RS17590 at 391.355 kb on - strand, within HMPREF1058_RS17590 at 391.355 kb on - strand, within HMPREF1058_RS17590 at 391.448 kb on + strand, within HMPREF1058_RS17590 at 391.448 kb on + strand, within HMPREF1058_RS17590 at 391.513 kb on + strand, within HMPREF1058_RS17590 at 391.513 kb on + strand, within HMPREF1058_RS17590 at 391.534 kb on - strand, within HMPREF1058_RS17590 at 391.577 kb on - strand, within HMPREF1058_RS17590 at 391.577 kb on - strand, within HMPREF1058_RS17590 at 391.578 kb on + strand, within HMPREF1058_RS17590 at 391.579 kb on - strand, within HMPREF1058_RS17590 at 391.582 kb on + strand, within HMPREF1058_RS17590 at 391.582 kb on + strand, within HMPREF1058_RS17590 at 391.582 kb on + strand, within HMPREF1058_RS17590 at 391.601 kb on - strand, within HMPREF1058_RS17590 at 391.631 kb on + strand, within HMPREF1058_RS17590 at 391.673 kb on + strand, within HMPREF1058_RS17590 at 391.673 kb on + strand, within HMPREF1058_RS17590 at 391.674 kb on - strand, within HMPREF1058_RS17590 at 391.715 kb on + strand, within HMPREF1058_RS17590 at 391.734 kb on + strand, within HMPREF1058_RS17590 at 391.747 kb on + strand, within HMPREF1058_RS17590 at 391.777 kb on + strand at 391.777 kb on + strand at 391.826 kb on - strand at 391.837 kb on - strand at 391.837 kb on - strand at 391.837 kb on - strand at 391.837 kb on - strand at 391.837 kb on - strand at 391.854 kb on - strand at 391.854 kb on - strand at 391.856 kb on + strand at 391.893 kb on - strand at 391.893 kb on - strand at 391.901 kb on - strand at 392.116 kb on - strand at 392.216 kb on - strand at 392.233 kb on + strand at 392.234 kb on - strand at 392.251 kb on - strand at 392.283 kb on + strand at 392.286 kb on - strand at 392.289 kb on + strand at 392.289 kb on + strand at 392.290 kb on - strand at 392.290 kb on - strand at 392.290 kb on - strand at 392.291 kb on + strand at 392.291 kb on + strand at 392.291 kb on + strand at 392.296 kb on - strand at 392.333 kb on - strand at 392.339 kb on + strand at 392.342 kb on - strand at 392.455 kb on - strand, within HMPREF1058_RS17585 at 392.480 kb on - strand, within HMPREF1058_RS17585 at 392.481 kb on + strand, within HMPREF1058_RS17585 at 392.548 kb on + strand, within HMPREF1058_RS17585 at 392.551 kb on + strand, within HMPREF1058_RS17585 at 392.552 kb on - strand, within HMPREF1058_RS17585 at 392.585 kb on - strand, within HMPREF1058_RS17585 at 392.585 kb on - strand, within HMPREF1058_RS17585 at 392.586 kb on + strand, within HMPREF1058_RS17585 at 392.614 kb on + strand, within HMPREF1058_RS17585 at 392.615 kb on - strand, within HMPREF1058_RS17585 at 392.617 kb on + strand, within HMPREF1058_RS17585 at 392.618 kb on - strand, within HMPREF1058_RS17585 at 392.658 kb on - strand, within HMPREF1058_RS17585 at 392.701 kb on + strand at 392.704 kb on - strand at 392.704 kb on - strand at 392.742 kb on - strand at 392.788 kb on - strand at 392.793 kb on - strand at 392.842 kb on - strand at 392.848 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Serine (N); Varel_Bryant_medium_Glucose_noMET_DTT_NaS_B12_noNitrogen remove 389,919 - HMPREF1058_RS17600 0.46 +0.6 389,945 - HMPREF1058_RS17600 0.53 +0.6 389,968 - HMPREF1058_RS17600 0.58 +0.4 389,993 + HMPREF1058_RS17600 0.64 +0.6 389,994 - HMPREF1058_RS17600 0.64 -1.8 390,008 + HMPREF1058_RS17600 0.67 -0.1 390,106 - +0.4 390,106 - +0.4 390,119 + +1.1 390,120 - +2.2 390,122 - -2.2 390,130 + +1.1 390,131 - -0.9 390,131 - +0.1 390,132 + +0.7 390,132 + -1.8 390,132 + +0.7 390,135 - -0.1 390,135 - +0.5 390,135 - -0.2 390,137 - -1.4 390,146 + -0.5 390,228 + +2.6 390,228 + +0.5 390,239 + +1.1 390,239 + +1.9 390,240 - +0.3 390,267 + +1.6 390,283 + +1.5 390,284 - +1.1 390,284 - -1.1 390,308 - -1.2 390,333 - -1.7 390,343 + -3.6 390,382 - -2.7 390,386 - HMPREF1058_RS17595 0.10 -0.9 390,386 - HMPREF1058_RS17595 0.10 -1.6 390,491 + HMPREF1058_RS17595 0.32 -3.6 390,491 + HMPREF1058_RS17595 0.32 -2.4 390,492 - HMPREF1058_RS17595 0.33 -0.2 390,567 + HMPREF1058_RS17595 0.49 -2.4 390,609 + HMPREF1058_RS17595 0.58 -1.5 390,630 + HMPREF1058_RS17595 0.62 -2.6 390,641 + HMPREF1058_RS17595 0.64 -3.4 390,642 - HMPREF1058_RS17595 0.65 -2.1 390,642 - HMPREF1058_RS17595 0.65 -0.7 390,666 - HMPREF1058_RS17595 0.70 -2.7 390,678 + HMPREF1058_RS17595 0.72 -0.9 390,698 + HMPREF1058_RS17595 0.76 -1.2 390,699 - HMPREF1058_RS17595 0.77 -4.8 390,806 + -0.7 390,811 - -1.8 390,853 + -0.1 390,864 + -0.2 390,865 - -0.0 390,869 - -0.7 390,869 - -1.5 390,882 - -2.2 390,905 + -2.8 390,906 - -0.4 390,906 - -2.8 390,909 + -0.4 390,910 - -1.1 390,989 + HMPREF1058_RS17590 0.15 +0.6 390,990 - HMPREF1058_RS17590 0.15 +0.3 390,992 - HMPREF1058_RS17590 0.15 +0.9 390,995 - HMPREF1058_RS17590 0.16 -0.0 391,053 + HMPREF1058_RS17590 0.21 -0.9 391,053 + HMPREF1058_RS17590 0.21 -1.8 391,054 - HMPREF1058_RS17590 0.21 -0.7 391,061 + HMPREF1058_RS17590 0.22 -1.4 391,095 - HMPREF1058_RS17590 0.25 +0.6 391,220 - HMPREF1058_RS17590 0.37 +1.2 391,354 + HMPREF1058_RS17590 0.50 +0.4 391,355 - HMPREF1058_RS17590 0.50 -0.5 391,355 - HMPREF1058_RS17590 0.50 -0.7 391,448 + HMPREF1058_RS17590 0.59 -0.2 391,448 + HMPREF1058_RS17590 0.59 +2.2 391,513 + HMPREF1058_RS17590 0.65 -0.5 391,513 + HMPREF1058_RS17590 0.65 +0.9 391,534 - HMPREF1058_RS17590 0.67 -1.0 391,577 - HMPREF1058_RS17590 0.71 -0.2 391,577 - HMPREF1058_RS17590 0.71 -0.2 391,578 + HMPREF1058_RS17590 0.72 -0.5 391,579 - HMPREF1058_RS17590 0.72 -1.9 391,582 + HMPREF1058_RS17590 0.72 -0.7 391,582 + HMPREF1058_RS17590 0.72 -0.3 391,582 + HMPREF1058_RS17590 0.72 -1.1 391,601 - HMPREF1058_RS17590 0.74 -0.0 391,631 + HMPREF1058_RS17590 0.77 -1.9 391,673 + HMPREF1058_RS17590 0.81 -2.7 391,673 + HMPREF1058_RS17590 0.81 +0.7 391,674 - HMPREF1058_RS17590 0.81 -1.2 391,715 + HMPREF1058_RS17590 0.85 -1.2 391,734 + HMPREF1058_RS17590 0.87 +0.1 391,747 + HMPREF1058_RS17590 0.88 +0.6 391,777 + +1.3 391,777 + -0.6 391,826 - -2.3 391,837 - -1.6 391,837 - -0.5 391,837 - +0.9 391,837 - +0.6 391,837 - +0.5 391,854 - -1.2 391,854 - -1.3 391,856 + +0.9 391,893 - -2.1 391,893 - +0.5 391,901 - -0.1 392,116 - +0.9 392,216 - +0.8 392,233 + -0.8 392,234 - +1.9 392,251 - +0.4 392,283 + -0.9 392,286 - +1.3 392,289 + +1.0 392,289 + +0.5 392,290 - +0.6 392,290 - +0.5 392,290 - +0.3 392,291 + +0.7 392,291 + -0.4 392,291 + +0.1 392,296 - -0.4 392,333 - +0.5 392,339 + -2.4 392,342 - +0.8 392,455 - HMPREF1058_RS17585 0.30 -1.1 392,480 - HMPREF1058_RS17585 0.37 +0.5 392,481 + HMPREF1058_RS17585 0.38 -0.6 392,548 + HMPREF1058_RS17585 0.56 +0.8 392,551 + HMPREF1058_RS17585 0.57 +2.7 392,552 - HMPREF1058_RS17585 0.58 -1.2 392,585 - HMPREF1058_RS17585 0.67 -0.4 392,585 - HMPREF1058_RS17585 0.67 -0.8 392,586 + HMPREF1058_RS17585 0.67 -0.4 392,614 + HMPREF1058_RS17585 0.75 +1.9 392,615 - HMPREF1058_RS17585 0.75 +0.3 392,617 + HMPREF1058_RS17585 0.76 -1.6 392,618 - HMPREF1058_RS17585 0.76 +0.3 392,658 - HMPREF1058_RS17585 0.88 -0.3 392,701 + +0.8 392,704 - +0.1 392,704 - -0.4 392,742 - -2.8 392,788 - +0.1 392,793 - -1.3 392,842 - -1.6 392,848 - +4.3
Or see this region's nucleotide sequence