Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01240 and HMPREF1058_RS01235 are separated by 20 nucleotides HMPREF1058_RS01235 and HMPREF1058_RS01230 are separated by 272 nucleotides HMPREF1058_RS01230 and HMPREF1058_RS01225 are separated by 158 nucleotides
HMPREF1058_RS01240: HMPREF1058_RS01240 - DEAD/DEAH box helicase, at 172,276 to 173,553
_RS01240
HMPREF1058_RS01235: HMPREF1058_RS01235 - phosphoserine phosphatase SerB, at 173,574 to 174,806
_RS01235
HMPREF1058_RS01230: HMPREF1058_RS01230 - redoxin domain-containing protein, at 175,079 to 175,573
_RS01230
HMPREF1058_RS01225: HMPREF1058_RS01225 - HAD family phosphatase, at 175,732 to 176,352
_RS01225
Position (kb)
173
174
175 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 172.599 kb on - strand, within HMPREF1058_RS01240 at 172.630 kb on + strand, within HMPREF1058_RS01240 at 172.631 kb on - strand, within HMPREF1058_RS01240 at 172.695 kb on + strand, within HMPREF1058_RS01240 at 172.695 kb on + strand, within HMPREF1058_RS01240 at 172.741 kb on + strand, within HMPREF1058_RS01240 at 172.742 kb on - strand, within HMPREF1058_RS01240 at 172.851 kb on + strand, within HMPREF1058_RS01240 at 172.852 kb on - strand, within HMPREF1058_RS01240 at 172.881 kb on + strand, within HMPREF1058_RS01240 at 172.881 kb on + strand, within HMPREF1058_RS01240 at 172.881 kb on + strand, within HMPREF1058_RS01240 at 172.882 kb on - strand at 172.882 kb on - strand, within HMPREF1058_RS01240 at 172.883 kb on + strand, within HMPREF1058_RS01240 at 172.883 kb on + strand, within HMPREF1058_RS01240 at 172.884 kb on - strand, within HMPREF1058_RS01240 at 172.972 kb on + strand, within HMPREF1058_RS01240 at 173.028 kb on - strand, within HMPREF1058_RS01240 at 173.043 kb on + strand, within HMPREF1058_RS01240 at 173.074 kb on - strand, within HMPREF1058_RS01240 at 173.120 kb on - strand, within HMPREF1058_RS01240 at 173.123 kb on + strand, within HMPREF1058_RS01240 at 173.160 kb on + strand, within HMPREF1058_RS01240 at 173.170 kb on + strand, within HMPREF1058_RS01240 at 173.290 kb on + strand, within HMPREF1058_RS01240 at 173.464 kb on - strand at 173.474 kb on - strand at 173.555 kb on + strand at 173.557 kb on + strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.558 kb on - strand at 173.559 kb on + strand at 173.559 kb on + strand at 173.560 kb on - strand at 173.649 kb on - strand at 173.649 kb on - strand at 173.676 kb on + strand at 173.752 kb on - strand, within HMPREF1058_RS01235 at 173.760 kb on - strand, within HMPREF1058_RS01235 at 173.760 kb on - strand, within HMPREF1058_RS01235 at 173.769 kb on + strand, within HMPREF1058_RS01235 at 173.769 kb on + strand, within HMPREF1058_RS01235 at 173.770 kb on - strand, within HMPREF1058_RS01235 at 173.816 kb on + strand, within HMPREF1058_RS01235 at 173.833 kb on - strand, within HMPREF1058_RS01235 at 173.833 kb on - strand, within HMPREF1058_RS01235 at 173.857 kb on - strand, within HMPREF1058_RS01235 at 173.893 kb on - strand, within HMPREF1058_RS01235 at 173.893 kb on - strand, within HMPREF1058_RS01235 at 173.897 kb on - strand, within HMPREF1058_RS01235 at 173.912 kb on + strand, within HMPREF1058_RS01235 at 173.913 kb on - strand, within HMPREF1058_RS01235 at 173.923 kb on + strand, within HMPREF1058_RS01235 at 173.924 kb on - strand, within HMPREF1058_RS01235 at 173.935 kb on + strand, within HMPREF1058_RS01235 at 173.936 kb on - strand, within HMPREF1058_RS01235 at 174.183 kb on - strand, within HMPREF1058_RS01235 at 174.196 kb on - strand, within HMPREF1058_RS01235 at 174.248 kb on + strand, within HMPREF1058_RS01235 at 174.248 kb on + strand, within HMPREF1058_RS01235 at 174.248 kb on + strand, within HMPREF1058_RS01235 at 174.357 kb on - strand, within HMPREF1058_RS01235 at 174.366 kb on + strand, within HMPREF1058_RS01235 at 174.405 kb on - strand, within HMPREF1058_RS01235 at 174.447 kb on + strand, within HMPREF1058_RS01235 at 174.447 kb on + strand at 174.448 kb on - strand, within HMPREF1058_RS01235 at 174.604 kb on - strand, within HMPREF1058_RS01235 at 174.795 kb on - strand at 174.797 kb on + strand at 174.807 kb on - strand at 174.825 kb on - strand at 174.843 kb on + strand at 174.945 kb on - strand at 175.059 kb on - strand at 175.066 kb on - strand at 175.077 kb on - strand at 175.077 kb on - strand at 175.114 kb on - strand at 175.201 kb on + strand, within HMPREF1058_RS01230 at 175.202 kb on - strand, within HMPREF1058_RS01230 at 175.204 kb on + strand, within HMPREF1058_RS01230 at 175.205 kb on - strand, within HMPREF1058_RS01230 at 175.249 kb on + strand, within HMPREF1058_RS01230 at 175.253 kb on + strand, within HMPREF1058_RS01230 at 175.253 kb on + strand, within HMPREF1058_RS01230 at 175.254 kb on - strand, within HMPREF1058_RS01230 at 175.254 kb on - strand, within HMPREF1058_RS01230 at 175.254 kb on - strand, within HMPREF1058_RS01230 at 175.258 kb on - strand, within HMPREF1058_RS01230 at 175.264 kb on - strand, within HMPREF1058_RS01230 at 175.280 kb on + strand, within HMPREF1058_RS01230 at 175.373 kb on + strand, within HMPREF1058_RS01230 at 175.373 kb on + strand, within HMPREF1058_RS01230 at 175.424 kb on - strand, within HMPREF1058_RS01230 at 175.461 kb on + strand, within HMPREF1058_RS01230 at 175.461 kb on + strand, within HMPREF1058_RS01230 at 175.472 kb on + strand, within HMPREF1058_RS01230 at 175.653 kb on + strand at 175.654 kb on - strand at 175.655 kb on + strand at 175.673 kb on + strand at 175.673 kb on + strand at 175.674 kb on - strand at 175.674 kb on - strand at 175.723 kb on - strand at 175.744 kb on - strand at 175.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_DTT_NaS_noNitrogen remove 172,599 - HMPREF1058_RS01240 0.25 +0.7 172,630 + HMPREF1058_RS01240 0.28 +0.2 172,631 - HMPREF1058_RS01240 0.28 +0.9 172,695 + HMPREF1058_RS01240 0.33 +0.6 172,695 + HMPREF1058_RS01240 0.33 +0.9 172,741 + HMPREF1058_RS01240 0.36 +0.1 172,742 - HMPREF1058_RS01240 0.36 +1.9 172,851 + HMPREF1058_RS01240 0.45 +0.2 172,852 - HMPREF1058_RS01240 0.45 -0.3 172,881 + HMPREF1058_RS01240 0.47 +0.5 172,881 + HMPREF1058_RS01240 0.47 +0.5 172,881 + HMPREF1058_RS01240 0.47 -0.1 172,882 - +0.7 172,882 - HMPREF1058_RS01240 0.47 +1.5 172,883 + HMPREF1058_RS01240 0.47 +0.9 172,883 + HMPREF1058_RS01240 0.47 +3.1 172,884 - HMPREF1058_RS01240 0.48 -0.2 172,972 + HMPREF1058_RS01240 0.54 +0.7 173,028 - HMPREF1058_RS01240 0.59 -0.0 173,043 + HMPREF1058_RS01240 0.60 +0.1 173,074 - HMPREF1058_RS01240 0.62 +0.7 173,120 - HMPREF1058_RS01240 0.66 +0.5 173,123 + HMPREF1058_RS01240 0.66 +0.7 173,160 + HMPREF1058_RS01240 0.69 +0.8 173,170 + HMPREF1058_RS01240 0.70 +0.7 173,290 + HMPREF1058_RS01240 0.79 +0.0 173,464 - +0.7 173,474 - +0.3 173,555 + -1.2 173,557 + +0.0 173,558 - +0.3 173,558 - +0.3 173,558 - -0.1 173,558 - +0.0 173,558 - -0.5 173,559 + -0.5 173,559 + +1.2 173,560 - -1.8 173,649 - -0.6 173,649 - +0.3 173,676 + -0.2 173,752 - HMPREF1058_RS01235 0.14 -2.2 173,760 - HMPREF1058_RS01235 0.15 -1.2 173,760 - HMPREF1058_RS01235 0.15 -1.1 173,769 + HMPREF1058_RS01235 0.16 -1.2 173,769 + HMPREF1058_RS01235 0.16 -1.4 173,770 - HMPREF1058_RS01235 0.16 -1.7 173,816 + HMPREF1058_RS01235 0.20 -2.5 173,833 - HMPREF1058_RS01235 0.21 -1.6 173,833 - HMPREF1058_RS01235 0.21 -1.3 173,857 - HMPREF1058_RS01235 0.23 -2.4 173,893 - HMPREF1058_RS01235 0.26 -0.4 173,893 - HMPREF1058_RS01235 0.26 -1.1 173,897 - HMPREF1058_RS01235 0.26 -1.8 173,912 + HMPREF1058_RS01235 0.27 -2.9 173,913 - HMPREF1058_RS01235 0.27 -1.0 173,923 + HMPREF1058_RS01235 0.28 +0.1 173,924 - HMPREF1058_RS01235 0.28 +1.5 173,935 + HMPREF1058_RS01235 0.29 -0.6 173,936 - HMPREF1058_RS01235 0.29 -0.5 174,183 - HMPREF1058_RS01235 0.49 -0.9 174,196 - HMPREF1058_RS01235 0.50 +0.9 174,248 + HMPREF1058_RS01235 0.55 +0.3 174,248 + HMPREF1058_RS01235 0.55 -0.2 174,248 + HMPREF1058_RS01235 0.55 +1.5 174,357 - HMPREF1058_RS01235 0.64 -1.8 174,366 + HMPREF1058_RS01235 0.64 -1.5 174,405 - HMPREF1058_RS01235 0.67 -2.1 174,447 + HMPREF1058_RS01235 0.71 -1.3 174,447 + -2.2 174,448 - HMPREF1058_RS01235 0.71 -1.0 174,604 - HMPREF1058_RS01235 0.84 -2.6 174,795 - +0.8 174,797 + -1.1 174,807 - -1.4 174,825 - -1.1 174,843 + +1.3 174,945 - +0.4 175,059 - -0.9 175,066 - +1.5 175,077 - +1.0 175,077 - +1.1 175,114 - +0.3 175,201 + HMPREF1058_RS01230 0.25 +1.1 175,202 - HMPREF1058_RS01230 0.25 +0.3 175,204 + HMPREF1058_RS01230 0.25 +1.8 175,205 - HMPREF1058_RS01230 0.25 -0.8 175,249 + HMPREF1058_RS01230 0.34 +0.5 175,253 + HMPREF1058_RS01230 0.35 -0.6 175,253 + HMPREF1058_RS01230 0.35 +0.5 175,254 - HMPREF1058_RS01230 0.35 +0.4 175,254 - HMPREF1058_RS01230 0.35 +0.4 175,254 - HMPREF1058_RS01230 0.35 +0.8 175,258 - HMPREF1058_RS01230 0.36 +0.6 175,264 - HMPREF1058_RS01230 0.37 -0.3 175,280 + HMPREF1058_RS01230 0.41 -0.5 175,373 + HMPREF1058_RS01230 0.59 -0.3 175,373 + HMPREF1058_RS01230 0.59 +0.7 175,424 - HMPREF1058_RS01230 0.70 +0.8 175,461 + HMPREF1058_RS01230 0.77 +0.1 175,461 + HMPREF1058_RS01230 0.77 +1.3 175,472 + HMPREF1058_RS01230 0.79 +0.0 175,653 + -0.4 175,654 - +1.1 175,655 + -0.1 175,673 + -0.5 175,673 + -1.5 175,674 - -1.8 175,674 - -0.4 175,723 - +0.4 175,744 - +0.5 175,749 + +1.3
Or see this region's nucleotide sequence