Experiment: L-Glutamine (N); Varel_Bryant_medium_Glucose_lowCys_noNitrogen
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06905 and HMPREF1058_RS06900 are separated by 117 nucleotides HMPREF1058_RS06900 and HMPREF1058_RS06895 are separated by 75 nucleotides HMPREF1058_RS06895 and HMPREF1058_RS06890 are separated by 57 nucleotides
HMPREF1058_RS06905: HMPREF1058_RS06905 - hypothetical protein, at 364,409 to 365,134
_RS06905
HMPREF1058_RS06900: HMPREF1058_RS06900 - P-II family nitrogen regulator, at 365,252 to 365,608
_RS06900
HMPREF1058_RS06895: HMPREF1058_RS06895 - ammonium transporter, at 365,684 to 367,042
_RS06895
HMPREF1058_RS06890: HMPREF1058_RS06890 - glutamine synthetase III, at 367,100 to 369,289
_RS06890
Position (kb) 365
366
367
368 Strain fitness (log2 ratio) -2
-1
0
1 at 364.776 kb on + strand, within HMPREF1058_RS06905 at 364.776 kb on + strand, within HMPREF1058_RS06905 at 364.863 kb on - strand, within HMPREF1058_RS06905 at 364.872 kb on - strand, within HMPREF1058_RS06905 at 364.875 kb on + strand, within HMPREF1058_RS06905 at 364.876 kb on - strand, within HMPREF1058_RS06905 at 364.876 kb on - strand, within HMPREF1058_RS06905 at 364.956 kb on - strand, within HMPREF1058_RS06905 at 364.956 kb on - strand, within HMPREF1058_RS06905 at 364.956 kb on - strand, within HMPREF1058_RS06905 at 364.956 kb on - strand, within HMPREF1058_RS06905 at 365.188 kb on + strand at 365.189 kb on - strand at 365.234 kb on + strand at 365.235 kb on - strand at 365.350 kb on - strand, within HMPREF1058_RS06900 at 365.381 kb on - strand, within HMPREF1058_RS06900 at 365.381 kb on - strand, within HMPREF1058_RS06900 at 365.399 kb on + strand, within HMPREF1058_RS06900 at 365.400 kb on - strand, within HMPREF1058_RS06900 at 365.692 kb on + strand at 365.694 kb on + strand at 365.700 kb on + strand at 365.701 kb on - strand at 365.701 kb on - strand at 365.823 kb on + strand, within HMPREF1058_RS06895 at 365.841 kb on + strand, within HMPREF1058_RS06895 at 365.842 kb on - strand, within HMPREF1058_RS06895 at 366.155 kb on + strand, within HMPREF1058_RS06895 at 366.165 kb on - strand, within HMPREF1058_RS06895 at 366.167 kb on - strand, within HMPREF1058_RS06895 at 366.167 kb on - strand, within HMPREF1058_RS06895 at 366.302 kb on - strand at 366.698 kb on - strand, within HMPREF1058_RS06895 at 366.727 kb on - strand, within HMPREF1058_RS06895 at 366.858 kb on - strand, within HMPREF1058_RS06895 at 367.035 kb on - strand at 367.035 kb on - strand at 367.100 kb on - strand at 367.229 kb on + strand at 367.731 kb on - strand, within HMPREF1058_RS06890 at 367.751 kb on + strand, within HMPREF1058_RS06890 at 367.752 kb on - strand, within HMPREF1058_RS06890 at 367.860 kb on - strand, within HMPREF1058_RS06890 at 367.860 kb on - strand, within HMPREF1058_RS06890 at 368.020 kb on + strand, within HMPREF1058_RS06890
Per-strain Table
Position Strand Gene LocusTag Fraction L-Glutamine (N); Varel_Bryant_medium_Glucose_lowCys_noNitrogen remove 364,776 + HMPREF1058_RS06905 0.51 -1.4 364,776 + HMPREF1058_RS06905 0.51 -1.2 364,863 - HMPREF1058_RS06905 0.63 -1.1 364,872 - HMPREF1058_RS06905 0.64 -1.0 364,875 + HMPREF1058_RS06905 0.64 +0.6 364,876 - HMPREF1058_RS06905 0.64 -0.8 364,876 - HMPREF1058_RS06905 0.64 -0.3 364,956 - HMPREF1058_RS06905 0.75 -1.1 364,956 - HMPREF1058_RS06905 0.75 -1.0 364,956 - HMPREF1058_RS06905 0.75 -1.1 364,956 - HMPREF1058_RS06905 0.75 -1.4 365,188 + -1.3 365,189 - -1.4 365,234 + -1.3 365,235 - -1.1 365,350 - HMPREF1058_RS06900 0.27 -1.6 365,381 - HMPREF1058_RS06900 0.36 -1.3 365,381 - HMPREF1058_RS06900 0.36 -1.0 365,399 + HMPREF1058_RS06900 0.41 -1.5 365,400 - HMPREF1058_RS06900 0.41 +0.2 365,692 + +0.2 365,694 + -1.2 365,700 + +0.9 365,701 - -1.2 365,701 - -1.5 365,823 + HMPREF1058_RS06895 0.10 -0.4 365,841 + HMPREF1058_RS06895 0.12 +0.9 365,842 - HMPREF1058_RS06895 0.12 +1.2 366,155 + HMPREF1058_RS06895 0.35 -0.4 366,165 - HMPREF1058_RS06895 0.35 -1.7 366,167 - HMPREF1058_RS06895 0.36 -1.7 366,167 - HMPREF1058_RS06895 0.36 -2.0 366,302 - +1.2 366,698 - HMPREF1058_RS06895 0.75 -1.1 366,727 - HMPREF1058_RS06895 0.77 -1.5 366,858 - HMPREF1058_RS06895 0.86 -1.6 367,035 - -1.5 367,035 - -1.8 367,100 - -1.3 367,229 + +0.2 367,731 - HMPREF1058_RS06890 0.29 -0.8 367,751 + HMPREF1058_RS06890 0.30 -2.1 367,752 - HMPREF1058_RS06890 0.30 -1.3 367,860 - HMPREF1058_RS06890 0.35 -0.4 367,860 - HMPREF1058_RS06890 0.35 -1.2 368,020 + HMPREF1058_RS06890 0.42 -1.8
Or see this region's nucleotide sequence