Experiment: Pectin from apple; autoclaved 2.5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS11965 and HMPREF1058_RS20655 are separated by 334 nucleotides HMPREF1058_RS20655 and HMPREF1058_RS11960 are separated by 113 nucleotides HMPREF1058_RS11960 and HMPREF1058_RS11955 are separated by 155 nucleotides HMPREF1058_RS11955 and HMPREF1058_RS11950 are separated by 27 nucleotides HMPREF1058_RS11950 and HMPREF1058_RS11945 overlap by 1 nucleotides
HMPREF1058_RS11965: HMPREF1058_RS11965 - glycoside hydrolase family 88 protein, at 234,690 to 235,988
_RS11965
HMPREF1058_RS20655: HMPREF1058_RS20655 - hypothetical protein, at 236,323 to 236,525
_RS20655
HMPREF1058_RS11960: HMPREF1058_RS11960 - tRNA-Ile, at 236,639 to 236,712
_RS11960
HMPREF1058_RS11955: HMPREF1058_RS11955 - calcium-translocating P-type ATPase, PMCA-type, at 236,868 to 239,498
_RS11955
HMPREF1058_RS11950: HMPREF1058_RS11950 - HAD family phosphatase, at 239,526 to 240,140
_RS11950
HMPREF1058_RS11945: HMPREF1058_RS11945 - bifunctional riboflavin kinase/FAD synthetase, at 240,140 to 241,060
_RS11945
Position (kb) 236
237
238
239
240 Strain fitness (log2 ratio) -1
0
1
2 at 235.892 kb on + strand at 235.988 kb on + strand at 235.989 kb on - strand at 235.989 kb on - strand at 235.989 kb on - strand at 236.017 kb on + strand at 236.065 kb on + strand at 236.065 kb on + strand at 236.082 kb on - strand at 236.160 kb on + strand at 236.161 kb on - strand at 236.231 kb on + strand at 236.260 kb on + strand at 236.270 kb on + strand at 236.295 kb on + strand at 236.299 kb on - strand at 236.539 kb on - strand at 236.539 kb on - strand at 236.541 kb on - strand at 236.541 kb on - strand at 236.542 kb on + strand at 236.542 kb on + strand at 236.542 kb on + strand at 236.575 kb on + strand at 236.575 kb on + strand at 236.576 kb on - strand at 236.576 kb on - strand at 236.576 kb on - strand at 236.578 kb on - strand at 236.578 kb on - strand at 236.824 kb on + strand at 239.509 kb on - strand at 239.517 kb on + strand at 239.517 kb on + strand at 239.518 kb on - strand at 239.535 kb on - strand at 239.631 kb on - strand, within HMPREF1058_RS11950 at 239.768 kb on + strand, within HMPREF1058_RS11950 at 239.785 kb on + strand, within HMPREF1058_RS11950 at 239.815 kb on + strand, within HMPREF1058_RS11950 at 239.815 kb on + strand, within HMPREF1058_RS11950 at 239.816 kb on - strand, within HMPREF1058_RS11950 at 239.816 kb on - strand, within HMPREF1058_RS11950 at 239.838 kb on + strand, within HMPREF1058_RS11950 at 239.838 kb on + strand, within HMPREF1058_RS11950 at 239.839 kb on - strand, within HMPREF1058_RS11950 at 239.840 kb on + strand, within HMPREF1058_RS11950 at 239.841 kb on - strand, within HMPREF1058_RS11950 at 239.858 kb on + strand, within HMPREF1058_RS11950 at 239.858 kb on + strand, within HMPREF1058_RS11950 at 239.858 kb on + strand, within HMPREF1058_RS11950 at 240.096 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Pectin from apple; autoclaved 2.5 mg/ml (C) remove 235,892 + +0.0 235,988 + +0.9 235,989 - -0.1 235,989 - -0.5 235,989 - +0.4 236,017 + +1.8 236,065 + -0.6 236,065 + +0.3 236,082 - -0.7 236,160 + +0.1 236,161 - -0.1 236,231 + -0.3 236,260 + -0.2 236,270 + -0.0 236,295 + +0.0 236,299 - -0.5 236,539 - -0.8 236,539 - +2.1 236,541 - -0.1 236,541 - +0.4 236,542 + -0.1 236,542 + +0.2 236,542 + +2.3 236,575 + +1.7 236,575 + -0.0 236,576 - -0.3 236,576 - +1.6 236,576 - -0.9 236,578 - -0.8 236,578 - -0.8 236,824 + +0.5 239,509 - -0.5 239,517 + +0.3 239,517 + +0.1 239,518 - -0.2 239,535 - -0.2 239,631 - HMPREF1058_RS11950 0.17 -0.3 239,768 + HMPREF1058_RS11950 0.39 +0.6 239,785 + HMPREF1058_RS11950 0.42 -1.5 239,815 + HMPREF1058_RS11950 0.47 +0.7 239,815 + HMPREF1058_RS11950 0.47 -0.1 239,816 - HMPREF1058_RS11950 0.47 -0.6 239,816 - HMPREF1058_RS11950 0.47 -1.3 239,838 + HMPREF1058_RS11950 0.51 -0.7 239,838 + HMPREF1058_RS11950 0.51 +0.2 239,839 - HMPREF1058_RS11950 0.51 -0.3 239,840 + HMPREF1058_RS11950 0.51 +0.2 239,841 - HMPREF1058_RS11950 0.51 -0.2 239,858 + HMPREF1058_RS11950 0.54 -0.2 239,858 + HMPREF1058_RS11950 0.54 -1.3 239,858 + HMPREF1058_RS11950 0.54 +1.2 240,096 + -0.2
Or see this region's nucleotide sequence