Experiment: H2 N-free with 10mM Cytidine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06635 and MMP_RS06640 are separated by 62 nucleotides MMP_RS06640 and MMP_RS06645 are separated by 125 nucleotides MMP_RS06645 and MMP_RS06650 are separated by 16 nucleotides
MMP_RS06635: MMP_RS06635 - UbiD family decarboxylase, at 1,271,031 to 1,272,308
_RS06635
MMP_RS06640: MMP_RS06640 - 50S ribosomal protein L16, at 1,272,371 to 1,272,892
_RS06640
MMP_RS06645: MMP_RS06645 - GTP-binding protein EngB, at 1,273,018 to 1,273,710
_RS06645
MMP_RS06650: MMP_RS06650 - glycoside hydrolase family 57 protein, at 1,273,727 to 1,274,950
_RS06650
Position (kb)
1273
1274 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1272.152 kb on - strand at 1272.325 kb on + strand at 1272.380 kb on + strand at 1272.674 kb on - strand at 1272.953 kb on + strand at 1273.058 kb on + strand at 1273.250 kb on + strand at 1273.365 kb on - strand at 1273.366 kb on + strand at 1273.466 kb on - strand at 1273.467 kb on + strand at 1273.712 kb on - strand at 1273.737 kb on - strand at 1273.878 kb on - strand, within MMP_RS06650 at 1273.912 kb on + strand, within MMP_RS06650 at 1273.925 kb on - strand, within MMP_RS06650 at 1273.926 kb on + strand, within MMP_RS06650 at 1273.977 kb on - strand, within MMP_RS06650 at 1274.111 kb on + strand, within MMP_RS06650 at 1274.182 kb on + strand, within MMP_RS06650 at 1274.258 kb on + strand, within MMP_RS06650 at 1274.342 kb on + strand, within MMP_RS06650 at 1274.388 kb on + strand, within MMP_RS06650 at 1274.438 kb on + strand, within MMP_RS06650 at 1274.527 kb on - strand, within MMP_RS06650 at 1274.561 kb on - strand, within MMP_RS06650 at 1274.585 kb on + strand, within MMP_RS06650
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM Cytidine remove 1,272,152 - -2.2 1,272,325 + +2.5 1,272,380 + -0.0 1,272,674 - -1.5 1,272,953 + +0.3 1,273,058 + -1.2 1,273,250 + -2.8 1,273,365 - -1.3 1,273,366 + -3.9 1,273,466 - -0.7 1,273,467 + -4.0 1,273,712 - +0.1 1,273,737 - +0.0 1,273,878 - MMP_RS06650 0.12 +0.9 1,273,912 + MMP_RS06650 0.15 -0.1 1,273,925 - MMP_RS06650 0.16 +1.1 1,273,926 + MMP_RS06650 0.16 -0.0 1,273,977 - MMP_RS06650 0.20 +2.8 1,274,111 + MMP_RS06650 0.31 +0.7 1,274,182 + MMP_RS06650 0.37 +1.7 1,274,258 + MMP_RS06650 0.43 +1.6 1,274,342 + MMP_RS06650 0.50 -0.1 1,274,388 + MMP_RS06650 0.54 +2.3 1,274,438 + MMP_RS06650 0.58 +2.5 1,274,527 - MMP_RS06650 0.65 +2.0 1,274,561 - MMP_RS06650 0.68 -0.1 1,274,585 + MMP_RS06650 0.70 -0.5
Or see this region's nucleotide sequence