Experiment: H2 N-free with 5mM Inosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04215 and MMP_RS04220 are separated by 48 nucleotides MMP_RS04220 and MMP_RS04225 are separated by 86 nucleotides MMP_RS04225 and MMP_RS04230 are separated by 80 nucleotides
MMP_RS04215: MMP_RS04215 - nickel pincer cofactor biosynthesis protein LarB, at 801,580 to 802,365
_RS04215
MMP_RS04220: MMP_RS04220 - queuosine precursor transporter, at 802,414 to 803,022
_RS04220
MMP_RS04225: MMP_RS04225 - hypothetical protein, at 803,109 to 803,621
_RS04225
MMP_RS04230: MMP_RS04230 - hypothetical protein, at 803,702 to 805,855
_RS04230
Position (kb) 803
804 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 802.265 kb on - strand, within MMP_RS04215 at 802.407 kb on + strand at 802.443 kb on + strand at 802.445 kb on + strand at 802.469 kb on + strand at 802.474 kb on + strand at 802.529 kb on + strand, within MMP_RS04220 at 802.587 kb on - strand, within MMP_RS04220 at 802.628 kb on - strand, within MMP_RS04220 at 802.660 kb on - strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.661 kb on + strand, within MMP_RS04220 at 802.680 kb on + strand, within MMP_RS04220 at 802.723 kb on - strand, within MMP_RS04220 at 802.748 kb on - strand, within MMP_RS04220 at 802.869 kb on - strand, within MMP_RS04220 at 802.879 kb on - strand, within MMP_RS04220 at 802.880 kb on + strand, within MMP_RS04220 at 802.883 kb on - strand, within MMP_RS04220 at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.971 kb on - strand at 802.972 kb on + strand at 802.972 kb on + strand at 803.016 kb on - strand at 803.032 kb on - strand at 803.033 kb on + strand at 803.036 kb on - strand at 803.036 kb on - strand at 803.037 kb on + strand at 803.046 kb on + strand at 803.081 kb on + strand at 803.126 kb on + strand at 803.130 kb on - strand at 803.151 kb on - strand at 803.152 kb on + strand at 803.152 kb on + strand at 803.164 kb on + strand, within MMP_RS04225 at 803.186 kb on - strand, within MMP_RS04225 at 803.196 kb on + strand, within MMP_RS04225 at 803.264 kb on - strand, within MMP_RS04225 at 803.265 kb on + strand, within MMP_RS04225 at 803.359 kb on - strand, within MMP_RS04225 at 803.405 kb on - strand, within MMP_RS04225 at 803.405 kb on - strand, within MMP_RS04225 at 803.476 kb on + strand, within MMP_RS04225 at 803.477 kb on - strand, within MMP_RS04225 at 803.622 kb on - strand at 803.622 kb on - strand at 803.623 kb on + strand at 803.623 kb on + strand at 803.624 kb on - strand at 803.624 kb on - strand at 803.624 kb on - strand at 803.643 kb on - strand at 803.643 kb on - strand at 803.659 kb on + strand at 803.663 kb on + strand at 803.663 kb on + strand at 803.663 kb on + strand at 803.663 kb on + strand at 803.664 kb on - strand at 803.694 kb on - strand at 803.694 kb on - strand at 803.695 kb on + strand at 803.703 kb on - strand at 803.714 kb on - strand at 803.714 kb on - strand at 803.715 kb on + strand at 803.715 kb on + strand at 803.715 kb on + strand at 803.826 kb on - strand at 803.827 kb on + strand at 803.852 kb on + strand at 803.865 kb on + strand at 803.921 kb on + strand, within MMP_RS04230 at 803.921 kb on + strand, within MMP_RS04230 at 803.946 kb on - strand, within MMP_RS04230 at 803.946 kb on - strand, within MMP_RS04230 at 803.946 kb on - strand, within MMP_RS04230 at 803.947 kb on + strand, within MMP_RS04230 at 804.080 kb on + strand, within MMP_RS04230 at 804.157 kb on - strand, within MMP_RS04230 at 804.173 kb on + strand, within MMP_RS04230 at 804.198 kb on - strand, within MMP_RS04230 at 804.200 kb on - strand, within MMP_RS04230 at 804.249 kb on + strand, within MMP_RS04230 at 804.296 kb on + strand, within MMP_RS04230 at 804.298 kb on - strand, within MMP_RS04230 at 804.424 kb on - strand, within MMP_RS04230 at 804.424 kb on - strand, within MMP_RS04230 at 804.515 kb on - strand, within MMP_RS04230 at 804.548 kb on - strand, within MMP_RS04230 at 804.607 kb on + strand, within MMP_RS04230 at 804.607 kb on + strand, within MMP_RS04230
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 5mM Inosine remove 802,265 - MMP_RS04215 0.87 -3.6 802,407 + -0.5 802,443 + +1.2 802,445 + -0.2 802,469 + +0.1 802,474 + -1.3 802,529 + MMP_RS04220 0.19 -1.3 802,587 - MMP_RS04220 0.28 -0.5 802,628 - MMP_RS04220 0.35 +0.3 802,660 - MMP_RS04220 0.40 +1.9 802,661 + MMP_RS04220 0.41 -1.0 802,661 + MMP_RS04220 0.41 +0.6 802,661 + MMP_RS04220 0.41 +0.1 802,661 + MMP_RS04220 0.41 +1.2 802,680 + MMP_RS04220 0.44 -0.4 802,723 - MMP_RS04220 0.51 +1.0 802,748 - MMP_RS04220 0.55 +1.1 802,869 - MMP_RS04220 0.75 +0.9 802,879 - MMP_RS04220 0.76 -0.5 802,880 + MMP_RS04220 0.77 -0.1 802,883 - MMP_RS04220 0.77 +0.9 802,971 - +0.3 802,971 - +0.6 802,971 - +0.3 802,971 - -0.3 802,971 - +0.5 802,972 + +0.7 802,972 + +1.3 803,016 - +0.4 803,032 - -2.0 803,033 + -0.2 803,036 - +0.3 803,036 - -0.6 803,037 + +0.6 803,046 + +0.6 803,081 + -0.2 803,126 + +1.5 803,130 - +1.1 803,151 - +0.3 803,152 + +0.6 803,152 + -0.8 803,164 + MMP_RS04225 0.11 +1.7 803,186 - MMP_RS04225 0.15 -1.3 803,196 + MMP_RS04225 0.17 +1.0 803,264 - MMP_RS04225 0.30 +0.5 803,265 + MMP_RS04225 0.30 +1.1 803,359 - MMP_RS04225 0.49 +4.2 803,405 - MMP_RS04225 0.58 +0.3 803,405 - MMP_RS04225 0.58 +2.4 803,476 + MMP_RS04225 0.72 -0.0 803,477 - MMP_RS04225 0.72 +0.9 803,622 - +2.4 803,622 - -0.6 803,623 + -0.1 803,623 + +0.1 803,624 - -0.2 803,624 - +0.5 803,624 - +0.9 803,643 - +0.3 803,643 - +2.7 803,659 + -1.6 803,663 + +1.6 803,663 + -0.3 803,663 + -0.2 803,663 + +0.7 803,664 - +0.6 803,694 - +0.8 803,694 - -0.5 803,695 + -2.1 803,703 - +1.6 803,714 - +0.5 803,714 - -0.0 803,715 + -2.1 803,715 + -0.0 803,715 + -0.4 803,826 - -1.2 803,827 + +1.6 803,852 + +1.0 803,865 + -0.1 803,921 + MMP_RS04230 0.10 -0.5 803,921 + MMP_RS04230 0.10 +1.1 803,946 - MMP_RS04230 0.11 -1.8 803,946 - MMP_RS04230 0.11 +0.3 803,946 - MMP_RS04230 0.11 -0.2 803,947 + MMP_RS04230 0.11 +0.3 804,080 + MMP_RS04230 0.18 -0.6 804,157 - MMP_RS04230 0.21 +0.6 804,173 + MMP_RS04230 0.22 +0.3 804,198 - MMP_RS04230 0.23 +0.6 804,200 - MMP_RS04230 0.23 +0.6 804,249 + MMP_RS04230 0.25 +0.7 804,296 + MMP_RS04230 0.28 -0.1 804,298 - MMP_RS04230 0.28 -1.1 804,424 - MMP_RS04230 0.34 +1.2 804,424 - MMP_RS04230 0.34 -1.9 804,515 - MMP_RS04230 0.38 -0.4 804,548 - MMP_RS04230 0.39 +0.9 804,607 + MMP_RS04230 0.42 -0.8 804,607 + MMP_RS04230 0.42 -1.3
Or see this region's nucleotide sequence