Experiment: H2 N-free with 5mM Inosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02500 and MMP_RS02505 are separated by 3 nucleotides MMP_RS02505 and MMP_RS02510 overlap by 17 nucleotides
MMP_RS02500: MMP_RS02500 - minichromosome maintenance protein MCM, at 465,802 to 467,826
_RS02500
MMP_RS02505: MMP_RS02505 - DUF2540 domain-containing protein, at 467,830 to 468,102
_RS02505
MMP_RS02510: MMP_RS02510 - site-specific integrase, at 468,086 to 469,072
_RS02510
Position (kb) 467
468
469 Strain fitness (log2 ratio) -1
0
1
2
3
4 at 466.888 kb on + strand, within MMP_RS02500 at 467.298 kb on + strand, within MMP_RS02500 at 467.326 kb on - strand, within MMP_RS02500 at 467.326 kb on - strand, within MMP_RS02500 at 467.327 kb on + strand, within MMP_RS02500 at 467.330 kb on - strand, within MMP_RS02500 at 467.331 kb on + strand, within MMP_RS02500 at 467.331 kb on + strand, within MMP_RS02500 at 467.527 kb on - strand, within MMP_RS02500 at 467.560 kb on - strand, within MMP_RS02500 at 468.093 kb on - strand at 468.230 kb on + strand, within MMP_RS02510 at 468.294 kb on + strand, within MMP_RS02510 at 468.395 kb on + strand, within MMP_RS02510 at 468.485 kb on - strand, within MMP_RS02510 at 468.486 kb on + strand, within MMP_RS02510 at 468.570 kb on - strand, within MMP_RS02510 at 468.570 kb on - strand, within MMP_RS02510 at 468.747 kb on + strand, within MMP_RS02510 at 468.915 kb on - strand, within MMP_RS02510 at 468.943 kb on + strand, within MMP_RS02510 at 469.011 kb on + strand at 469.054 kb on - strand at 469.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 5mM Inosine remove 466,888 + MMP_RS02500 0.54 +0.1 467,298 + MMP_RS02500 0.74 -0.4 467,326 - MMP_RS02500 0.75 +1.5 467,326 - MMP_RS02500 0.75 +0.3 467,327 + MMP_RS02500 0.75 +1.4 467,330 - MMP_RS02500 0.75 +4.4 467,331 + MMP_RS02500 0.76 -0.7 467,331 + MMP_RS02500 0.76 +2.7 467,527 - MMP_RS02500 0.85 -1.4 467,560 - MMP_RS02500 0.87 -0.1 468,093 - +1.7 468,230 + MMP_RS02510 0.15 -0.1 468,294 + MMP_RS02510 0.21 -1.5 468,395 + MMP_RS02510 0.31 +0.9 468,485 - MMP_RS02510 0.40 +0.7 468,486 + MMP_RS02510 0.41 +0.4 468,570 - MMP_RS02510 0.49 +0.3 468,570 - MMP_RS02510 0.49 +0.2 468,747 + MMP_RS02510 0.67 -0.7 468,915 - MMP_RS02510 0.84 +2.6 468,943 + MMP_RS02510 0.87 -0.2 469,011 + +1.0 469,054 - -0.3 469,081 + -1.5
Or see this region's nucleotide sequence