Experiment: H2 N-free with 5mM Inosine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01425 and MMP_RS01430 are separated by 17 nucleotides MMP_RS01430 and MMP_RS01435 are separated by 35 nucleotides MMP_RS01435 and MMP_RS01440 are separated by 39 nucleotides
MMP_RS01425: MMP_RS01425 - tyrosine--tRNA ligase, at 263,898 to 264,827
_RS01425
MMP_RS01430: MMP_RS01430 - mechanosensitive ion channel family protein, at 264,845 to 265,900
_RS01430
MMP_RS01435: MMP_RS01435 - DMT family transporter, at 265,936 to 266,379
_RS01435
MMP_RS01440: MMP_RS01440 - NAAT family transporter, at 266,419 to 267,060
_RS01440
Position (kb) 264
265
266 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 263.902 kb on - strand at 263.909 kb on - strand at 264.233 kb on - strand at 264.238 kb on - strand at 264.238 kb on - strand at 264.291 kb on + strand at 264.341 kb on + strand at 264.368 kb on + strand at 264.512 kb on - strand at 264.547 kb on - strand at 264.547 kb on - strand at 264.942 kb on + strand at 265.180 kb on + strand, within MMP_RS01430 at 265.222 kb on - strand, within MMP_RS01430 at 265.495 kb on - strand, within MMP_RS01430 at 265.501 kb on - strand, within MMP_RS01430 at 265.502 kb on + strand, within MMP_RS01430 at 265.534 kb on - strand, within MMP_RS01430 at 265.535 kb on + strand, within MMP_RS01430 at 265.602 kb on - strand, within MMP_RS01430 at 265.603 kb on + strand, within MMP_RS01430 at 265.648 kb on + strand, within MMP_RS01430 at 265.881 kb on - strand at 265.881 kb on - strand at 265.882 kb on + strand at 265.883 kb on - strand at 265.884 kb on + strand at 265.884 kb on + strand at 266.003 kb on - strand, within MMP_RS01435 at 266.016 kb on + strand, within MMP_RS01435 at 266.141 kb on - strand, within MMP_RS01435 at 266.204 kb on - strand, within MMP_RS01435 at 266.325 kb on - strand, within MMP_RS01435 at 266.346 kb on + strand at 266.500 kb on - strand, within MMP_RS01440
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 5mM Inosine remove 263,902 - -2.1 263,909 - -1.3 264,233 - -2.9 264,238 - -1.7 264,238 - +0.3 264,291 + -2.5 264,341 + -1.0 264,368 + -2.1 264,512 - -3.3 264,547 - -1.3 264,547 - -1.2 264,942 + +2.7 265,180 + MMP_RS01430 0.32 +1.2 265,222 - MMP_RS01430 0.36 +2.1 265,495 - MMP_RS01430 0.62 +2.7 265,501 - MMP_RS01430 0.62 +2.2 265,502 + MMP_RS01430 0.62 +1.7 265,534 - MMP_RS01430 0.65 +0.7 265,535 + MMP_RS01430 0.65 +1.6 265,602 - MMP_RS01430 0.72 +2.5 265,603 + MMP_RS01430 0.72 +2.9 265,648 + MMP_RS01430 0.76 +1.1 265,881 - +2.7 265,881 - +1.8 265,882 + +3.0 265,883 - +1.7 265,884 + +0.8 265,884 + -0.3 266,003 - MMP_RS01435 0.15 +3.2 266,016 + MMP_RS01435 0.18 +2.7 266,141 - MMP_RS01435 0.46 +1.3 266,204 - MMP_RS01435 0.60 +1.8 266,325 - MMP_RS01435 0.88 +1.8 266,346 + +0.8 266,500 - MMP_RS01440 0.13 -1.0
Or see this region's nucleotide sequence