Experiment: H2 N-free with 10mM NAG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08245 and MMP_RS08250 are separated by 109 nucleotides MMP_RS08250 and MMP_RS08255 are separated by 31 nucleotides
MMP_RS08245: MMP_RS08245 - DUF4013 domain-containing protein, at 1,551,417 to 1,552,286
_RS08245
MMP_RS08250: MMP_RS08250 - pyruvate kinase, at 1,552,396 to 1,553,739
_RS08250
MMP_RS08255: MMP_RS08255 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC, at 1,553,771 to 1,554,961
_RS08255
Position (kb)
1552
1553
1554 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1551.415 kb on + strand at 1551.609 kb on - strand, within MMP_RS08245 at 1551.610 kb on + strand, within MMP_RS08245 at 1551.610 kb on + strand, within MMP_RS08245 at 1551.682 kb on + strand, within MMP_RS08245 at 1551.769 kb on - strand, within MMP_RS08245 at 1551.770 kb on + strand, within MMP_RS08245 at 1551.770 kb on + strand, within MMP_RS08245 at 1551.864 kb on - strand, within MMP_RS08245 at 1551.865 kb on + strand, within MMP_RS08245 at 1551.894 kb on - strand, within MMP_RS08245 at 1552.036 kb on - strand, within MMP_RS08245 at 1552.107 kb on + strand, within MMP_RS08245 at 1552.271 kb on + strand at 1552.271 kb on + strand at 1552.314 kb on + strand at 1552.328 kb on + strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.329 kb on - strand at 1552.330 kb on + strand at 1552.330 kb on + strand at 1552.333 kb on - strand at 1552.361 kb on - strand at 1552.361 kb on - strand at 1552.361 kb on - strand at 1552.362 kb on + strand at 1552.362 kb on + strand at 1552.362 kb on + strand at 1552.365 kb on - strand at 1552.365 kb on - strand at 1552.369 kb on - strand at 1552.380 kb on + strand at 1552.444 kb on + strand at 1552.561 kb on + strand, within MMP_RS08250 at 1552.681 kb on - strand, within MMP_RS08250 at 1552.770 kb on + strand, within MMP_RS08250 at 1552.799 kb on - strand, within MMP_RS08250 at 1552.863 kb on - strand, within MMP_RS08250 at 1552.866 kb on + strand, within MMP_RS08250 at 1552.970 kb on + strand, within MMP_RS08250 at 1553.125 kb on + strand, within MMP_RS08250 at 1553.159 kb on - strand, within MMP_RS08250 at 1553.164 kb on + strand, within MMP_RS08250 at 1553.193 kb on + strand, within MMP_RS08250 at 1553.398 kb on - strand, within MMP_RS08250 at 1553.441 kb on - strand, within MMP_RS08250 at 1553.456 kb on - strand, within MMP_RS08250 at 1553.456 kb on - strand, within MMP_RS08250 at 1553.457 kb on + strand, within MMP_RS08250 at 1553.457 kb on + strand, within MMP_RS08250 at 1553.622 kb on + strand at 1553.653 kb on + strand at 1553.672 kb on + strand at 1553.721 kb on + strand at 1553.736 kb on + strand at 1554.027 kb on + strand at 1554.031 kb on - strand at 1554.154 kb on + strand at 1554.349 kb on - strand at 1554.733 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM NAG remove 1,551,415 + +0.5 1,551,609 - MMP_RS08245 0.22 +1.3 1,551,610 + MMP_RS08245 0.22 +0.4 1,551,610 + MMP_RS08245 0.22 +0.4 1,551,682 + MMP_RS08245 0.30 +1.1 1,551,769 - MMP_RS08245 0.40 +1.2 1,551,770 + MMP_RS08245 0.41 +1.1 1,551,770 + MMP_RS08245 0.41 -1.4 1,551,864 - MMP_RS08245 0.51 +0.1 1,551,865 + MMP_RS08245 0.51 +0.1 1,551,894 - MMP_RS08245 0.55 -0.4 1,552,036 - MMP_RS08245 0.71 +0.7 1,552,107 + MMP_RS08245 0.79 -0.4 1,552,271 + +1.2 1,552,271 + +2.5 1,552,314 + +1.4 1,552,328 + +2.0 1,552,329 - -0.3 1,552,329 - -2.0 1,552,329 - -1.5 1,552,329 - +1.5 1,552,330 + -1.1 1,552,330 + +1.3 1,552,333 - +0.5 1,552,361 - +0.7 1,552,361 - +1.1 1,552,361 - +0.3 1,552,362 + +1.0 1,552,362 + +0.7 1,552,362 + +0.4 1,552,365 - +1.1 1,552,365 - -0.1 1,552,369 - -0.8 1,552,380 + +0.7 1,552,444 + -0.4 1,552,561 + MMP_RS08250 0.12 +0.4 1,552,681 - MMP_RS08250 0.21 +1.2 1,552,770 + MMP_RS08250 0.28 +0.9 1,552,799 - MMP_RS08250 0.30 +0.3 1,552,863 - MMP_RS08250 0.35 +1.0 1,552,866 + MMP_RS08250 0.35 +0.4 1,552,970 + MMP_RS08250 0.43 +1.3 1,553,125 + MMP_RS08250 0.54 -0.7 1,553,159 - MMP_RS08250 0.57 -0.4 1,553,164 + MMP_RS08250 0.57 +0.8 1,553,193 + MMP_RS08250 0.59 +0.8 1,553,398 - MMP_RS08250 0.75 +1.4 1,553,441 - MMP_RS08250 0.78 +1.7 1,553,456 - MMP_RS08250 0.79 +1.0 1,553,456 - MMP_RS08250 0.79 +1.1 1,553,457 + MMP_RS08250 0.79 +0.9 1,553,457 + MMP_RS08250 0.79 -0.1 1,553,622 + -0.0 1,553,653 + +0.4 1,553,672 + +0.0 1,553,721 + -0.7 1,553,736 + +1.3 1,554,027 + -3.0 1,554,031 - -1.9 1,554,154 + -1.9 1,554,349 - +0.5 1,554,733 + -1.4
Or see this region's nucleotide sequence