Strain Fitness in Methanococcus maripaludis S2 around MMP_RS05750

Experiment: H2 N-free with 10mM NAG

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMP_RS05740 and MMP_RS05745 are separated by 4 nucleotidesMMP_RS05745 and MMP_RS05750 are separated by 60 nucleotidesMMP_RS05750 and MMP_RS05755 are separated by 56 nucleotidesMMP_RS05755 and MMP_RS05760 are separated by 13 nucleotides MMP_RS05740: MMP_RS05740 - oligosaccharide repeat unit polymerase family protein, at 1,101,329 to 1,102,402 _RS05740 MMP_RS05745: MMP_RS05745 - hypothetical protein, at 1,102,407 to 1,103,162 _RS05745 MMP_RS05750: MMP_RS05750 - transketolase family protein, at 1,103,223 to 1,104,161 _RS05750 MMP_RS05755: MMP_RS05755 - ribulose-phosphate 3-epimerase, at 1,104,218 to 1,104,877 _RS05755 MMP_RS05760: MMP_RS05760 - transketolase, at 1,104,891 to 1,105,718 _RS05760 Position (kb) 1103 1104 1105Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 1102.576 kb on + strandat 1102.739 kb on - strandat 1102.740 kb on + strandat 1102.740 kb on + strandat 1102.750 kb on + strandat 1102.787 kb on - strandat 1102.830 kb on + strandat 1102.932 kb on - strandat 1103.115 kb on - strandat 1103.195 kb on - strandat 1103.207 kb on + strandat 1103.207 kb on + strandat 1103.537 kb on - strandat 1103.676 kb on - strandat 1104.081 kb on - strandat 1104.162 kb on + strandat 1104.163 kb on - strandat 1104.597 kb on - strandat 1104.708 kb on + strandat 1104.746 kb on - strandat 1104.991 kb on + strandat 1105.093 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 N-free with 10mM NAG
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1,102,576 + -2.1
1,102,739 - -2.1
1,102,740 + -1.8
1,102,740 + +0.5
1,102,750 + -2.3
1,102,787 - -0.5
1,102,830 + -3.3
1,102,932 - -2.0
1,103,115 - -2.8
1,103,195 - -0.9
1,103,207 + -4.1
1,103,207 + -2.8
1,103,537 - -1.9
1,103,676 - -3.3
1,104,081 - -3.6
1,104,162 + -0.8
1,104,163 - +0.5
1,104,597 - -2.1
1,104,708 + -1.8
1,104,746 - -0.4
1,104,991 + +0.3
1,105,093 + -2.7

Or see this region's nucleotide sequence