Experiment: H2 N-free with 10mM NAG
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03035 and MMP_RS03040 are separated by 9 nucleotides MMP_RS03040 and MMP_RS03045 are separated by 79 nucleotides MMP_RS03045 and MMP_RS03050 are separated by 99 nucleotides MMP_RS03050 and MMP_RS03055 are separated by 9 nucleotides
MMP_RS03035: MMP_RS03035 - 4-hydroxy-tetrahydrodipicolinate synthase, at 573,961 to 574,830
_RS03035
MMP_RS03040: MMP_RS03040 - 30S ribosomal protein S17e, at 574,840 to 575,031
_RS03040
MMP_RS03045: MMP_RS03045 - chorismate mutase, at 575,111 to 575,401
_RS03045
MMP_RS03050: MMP_RS03050 - DUF115 domain-containing protein, at 575,501 to 576,217
_RS03050
MMP_RS03055: MMP_RS03055 - anaerobic ribonucleoside-triphosphate reductase activating protein, at 576,227 to 576,937
_RS03055
Position (kb) 575
576 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 574.291 kb on - strand, within MMP_RS03035 at 574.365 kb on - strand, within MMP_RS03035 at 574.440 kb on + strand, within MMP_RS03035 at 574.441 kb on - strand, within MMP_RS03035 at 574.447 kb on - strand, within MMP_RS03035 at 574.447 kb on - strand, within MMP_RS03035 at 574.518 kb on + strand, within MMP_RS03035 at 575.050 kb on - strand at 575.094 kb on - strand at 575.096 kb on - strand at 575.192 kb on - strand at 575.333 kb on - strand at 575.402 kb on + strand at 575.402 kb on + strand at 575.451 kb on - strand at 575.490 kb on + strand at 575.644 kb on + strand at 575.737 kb on + strand at 576.093 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM NAG remove 574,291 - MMP_RS03035 0.38 -1.2 574,365 - MMP_RS03035 0.46 -3.8 574,440 + MMP_RS03035 0.55 -2.0 574,441 - MMP_RS03035 0.55 +0.2 574,447 - MMP_RS03035 0.56 -3.0 574,447 - MMP_RS03035 0.56 -3.0 574,518 + MMP_RS03035 0.64 -1.6 575,050 - +0.6 575,094 - -0.6 575,096 - +0.1 575,192 - -3.2 575,333 - -2.6 575,402 + -3.4 575,402 + -3.3 575,451 - -1.9 575,490 + -0.8 575,644 + -2.3 575,737 + -2.4 576,093 + -1.2
Or see this region's nucleotide sequence