Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08985 and MMP_RS08770 are separated by 22 nucleotides MMP_RS08770 and MMP_RS08775 overlap by 4 nucleotides MMP_RS08775 and MMP_RS08780 are separated by 9 nucleotides MMP_RS08780 and MMP_RS08785 are separated by 5 nucleotides MMP_RS08785 and MMP_RS08790 are separated by 8 nucleotides
MMP_RS08985: MMP_RS08985 - tRNA-Sec, at 1,644,096 to 1,644,185
_RS08985
MMP_RS08770: MMP_RS08770 - hypothetical protein, at 1,644,208 to 1,645,104
_RS08770
MMP_RS08775: MMP_RS08775 - methanogenesis marker 9 domain-containing protein, at 1,645,101 to 1,645,556
_RS08775
MMP_RS08780: MMP_RS08780 - 2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase, at 1,645,566 to 1,646,231
_RS08780
MMP_RS08785: MMP_RS08785 - RNA-protein complex protein Nop10, at 1,646,237 to 1,646,392
_RS08785
MMP_RS08790: MMP_RS08790 - translation initiation factor IF-2 subunit alpha, at 1,646,401 to 1,647,198
_RS08790
Position (kb)
1645
1646 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1644.261 kb on + strand at 1644.288 kb on - strand at 1644.408 kb on + strand, within MMP_RS08770 at 1644.417 kb on + strand, within MMP_RS08770 at 1644.441 kb on - strand, within MMP_RS08770 at 1644.563 kb on - strand, within MMP_RS08770 at 1644.571 kb on + strand, within MMP_RS08770 at 1644.683 kb on - strand, within MMP_RS08770 at 1644.684 kb on + strand, within MMP_RS08770 at 1644.710 kb on - strand, within MMP_RS08770 at 1644.769 kb on + strand, within MMP_RS08770 at 1644.861 kb on + strand, within MMP_RS08770 at 1644.867 kb on - strand, within MMP_RS08770 at 1644.870 kb on + strand, within MMP_RS08770 at 1645.014 kb on - strand, within MMP_RS08770 at 1645.088 kb on - strand at 1645.274 kb on + strand, within MMP_RS08775 at 1645.274 kb on + strand, within MMP_RS08775 at 1645.311 kb on + strand, within MMP_RS08775 at 1645.416 kb on - strand, within MMP_RS08775 at 1645.513 kb on + strand at 1645.556 kb on - strand at 1645.556 kb on - strand at 1645.559 kb on + strand at 1645.567 kb on - strand at 1645.740 kb on - strand at 1645.740 kb on - strand at 1645.804 kb on + strand at 1645.804 kb on + strand at 1645.838 kb on - strand at 1645.850 kb on - strand at 1645.851 kb on + strand at 1645.917 kb on - strand at 1645.919 kb on - strand at 1645.967 kb on + strand at 1645.980 kb on + strand at 1646.021 kb on + strand at 1646.072 kb on - strand at 1646.095 kb on - strand at 1646.175 kb on - strand at 1646.175 kb on - strand at 1646.231 kb on - strand at 1646.277 kb on - strand, within MMP_RS08785 at 1646.524 kb on - strand, within MMP_RS08790 at 1646.550 kb on + strand, within MMP_RS08790
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,644,261 + -0.9 1,644,288 - +0.5 1,644,408 + MMP_RS08770 0.22 -0.1 1,644,417 + MMP_RS08770 0.23 -0.9 1,644,441 - MMP_RS08770 0.26 +0.9 1,644,563 - MMP_RS08770 0.40 -0.3 1,644,571 + MMP_RS08770 0.40 +0.7 1,644,683 - MMP_RS08770 0.53 +1.0 1,644,684 + MMP_RS08770 0.53 -0.0 1,644,710 - MMP_RS08770 0.56 +1.1 1,644,769 + MMP_RS08770 0.63 +1.5 1,644,861 + MMP_RS08770 0.73 +0.6 1,644,867 - MMP_RS08770 0.73 +0.8 1,644,870 + MMP_RS08770 0.74 -0.3 1,645,014 - MMP_RS08770 0.90 -0.1 1,645,088 - +1.0 1,645,274 + MMP_RS08775 0.38 +1.2 1,645,274 + MMP_RS08775 0.38 +0.5 1,645,311 + MMP_RS08775 0.46 +0.4 1,645,416 - MMP_RS08775 0.69 +1.0 1,645,513 + -1.3 1,645,556 - +0.2 1,645,556 - -1.6 1,645,559 + +0.5 1,645,567 - -0.7 1,645,740 - -3.3 1,645,740 - -3.3 1,645,804 + -1.3 1,645,804 + -4.1 1,645,838 - -3.5 1,645,850 - -3.2 1,645,851 + -2.9 1,645,917 - -3.1 1,645,919 - -3.7 1,645,967 + -3.5 1,645,980 + -0.7 1,646,021 + -1.6 1,646,072 - -2.3 1,646,095 - -1.6 1,646,175 - -1.7 1,646,175 - -5.3 1,646,231 - -1.8 1,646,277 - MMP_RS08785 0.26 -1.8 1,646,524 - MMP_RS08790 0.15 +0.1 1,646,550 + MMP_RS08790 0.19 -4.1
Or see this region's nucleotide sequence