Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08590 and MMP_RS08595 are separated by 10 nucleotides MMP_RS08595 and MMP_RS08600 overlap by 11 nucleotides MMP_RS08600 and MMP_RS08605 are separated by 27 nucleotides MMP_RS08605 and MMP_RS08610 overlap by 11 nucleotides MMP_RS08610 and MMP_RS08615 are separated by 40 nucleotides
MMP_RS08590: MMP_RS08590 - flagellin, at 1,611,319 to 1,611,951
_RS08590
MMP_RS08595: MMP_RS08595 - flagellar protein C, at 1,611,962 to 1,612,522
_RS08595
MMP_RS08600: MMP_RS08600 - flagellar protein D, at 1,612,512 to 1,613,480
_RS08600
MMP_RS08605: MMP_RS08605 - flagellar protein E, at 1,613,508 to 1,613,915
_RS08605
MMP_RS08610: MMP_RS08610 - flagellar protein F, at 1,613,905 to 1,614,312
_RS08610
MMP_RS08615: MMP_RS08615 - flagellar protein G, at 1,614,353 to 1,614,805
_RS08615
Position (kb) 1612
1613
1614 Strain fitness (log2 ratio) -1
0
1
2 at 1611.541 kb on + strand, within MMP_RS08590 at 1611.699 kb on - strand, within MMP_RS08590 at 1611.816 kb on + strand, within MMP_RS08590 at 1611.818 kb on + strand, within MMP_RS08590 at 1611.822 kb on - strand, within MMP_RS08590 at 1611.920 kb on - strand at 1611.958 kb on - strand at 1611.962 kb on + strand at 1611.994 kb on + strand at 1612.250 kb on + strand, within MMP_RS08595 at 1612.426 kb on - strand, within MMP_RS08595 at 1612.854 kb on - strand, within MMP_RS08600 at 1612.855 kb on + strand, within MMP_RS08600 at 1613.230 kb on + strand, within MMP_RS08600 at 1613.362 kb on - strand, within MMP_RS08600 at 1613.438 kb on + strand at 1613.447 kb on + strand at 1613.492 kb on + strand at 1613.607 kb on + strand, within MMP_RS08605 at 1613.620 kb on + strand, within MMP_RS08605 at 1613.713 kb on - strand, within MMP_RS08605 at 1613.714 kb on + strand, within MMP_RS08605 at 1613.806 kb on - strand, within MMP_RS08605 at 1613.807 kb on + strand, within MMP_RS08605 at 1613.821 kb on - strand, within MMP_RS08605 at 1613.828 kb on - strand, within MMP_RS08605 at 1613.957 kb on - strand, within MMP_RS08610 at 1613.958 kb on + strand, within MMP_RS08610 at 1613.968 kb on - strand, within MMP_RS08610 at 1613.969 kb on + strand, within MMP_RS08610 at 1613.969 kb on + strand, within MMP_RS08610 at 1613.974 kb on - strand, within MMP_RS08610 at 1613.974 kb on - strand, within MMP_RS08610 at 1613.974 kb on - strand, within MMP_RS08610 at 1613.975 kb on + strand, within MMP_RS08610 at 1614.006 kb on + strand, within MMP_RS08610 at 1614.050 kb on - strand, within MMP_RS08610 at 1614.051 kb on + strand, within MMP_RS08610 at 1614.072 kb on + strand, within MMP_RS08610 at 1614.096 kb on - strand, within MMP_RS08610 at 1614.113 kb on - strand, within MMP_RS08610 at 1614.114 kb on + strand, within MMP_RS08610 at 1614.161 kb on + strand, within MMP_RS08610 at 1614.296 kb on + strand at 1614.298 kb on - strand at 1614.299 kb on + strand at 1614.351 kb on + strand at 1614.381 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,611,541 + MMP_RS08590 0.35 -0.3 1,611,699 - MMP_RS08590 0.60 -1.0 1,611,816 + MMP_RS08590 0.79 -0.4 1,611,818 + MMP_RS08590 0.79 +1.3 1,611,822 - MMP_RS08590 0.79 +2.4 1,611,920 - -0.2 1,611,958 - +1.5 1,611,962 + -0.6 1,611,994 + -0.3 1,612,250 + MMP_RS08595 0.51 +0.8 1,612,426 - MMP_RS08595 0.83 -0.2 1,612,854 - MMP_RS08600 0.35 +1.1 1,612,855 + MMP_RS08600 0.35 +0.1 1,613,230 + MMP_RS08600 0.74 +0.6 1,613,362 - MMP_RS08600 0.88 +0.9 1,613,438 + -0.1 1,613,447 + -0.0 1,613,492 + +0.5 1,613,607 + MMP_RS08605 0.24 -0.1 1,613,620 + MMP_RS08605 0.27 +0.0 1,613,713 - MMP_RS08605 0.50 -0.1 1,613,714 + MMP_RS08605 0.50 +0.4 1,613,806 - MMP_RS08605 0.73 -0.4 1,613,807 + MMP_RS08605 0.73 -0.7 1,613,821 - MMP_RS08605 0.77 +1.0 1,613,828 - MMP_RS08605 0.78 -0.2 1,613,957 - MMP_RS08610 0.13 +0.9 1,613,958 + MMP_RS08610 0.13 -1.4 1,613,968 - MMP_RS08610 0.15 +1.0 1,613,969 + MMP_RS08610 0.16 +1.0 1,613,969 + MMP_RS08610 0.16 +0.4 1,613,974 - MMP_RS08610 0.17 +0.4 1,613,974 - MMP_RS08610 0.17 -0.5 1,613,974 - MMP_RS08610 0.17 -0.6 1,613,975 + MMP_RS08610 0.17 -0.7 1,614,006 + MMP_RS08610 0.25 +0.1 1,614,050 - MMP_RS08610 0.36 +0.4 1,614,051 + MMP_RS08610 0.36 +0.8 1,614,072 + MMP_RS08610 0.41 -0.7 1,614,096 - MMP_RS08610 0.47 -0.1 1,614,113 - MMP_RS08610 0.51 -0.2 1,614,114 + MMP_RS08610 0.51 +0.9 1,614,161 + MMP_RS08610 0.63 +0.3 1,614,296 + +0.0 1,614,298 - +0.6 1,614,299 + +0.2 1,614,351 + -0.6 1,614,381 - -0.5
Or see this region's nucleotide sequence