Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08545 and MMP_RS08550 are separated by 85 nucleotides MMP_RS08550 and MMP_RS08555 overlap by 11 nucleotides MMP_RS08555 and MMP_RS08560 overlap by 4 nucleotides MMP_RS08560 and MMP_RS08565 are separated by 29 nucleotides
MMP_RS08545: MMP_RS08545 - aspartate carbamoyltransferase, at 1,603,462 to 1,604,370
_RS08545
MMP_RS08550: MMP_RS08550 - DUF2304 domain-containing protein, at 1,604,456 to 1,604,815
_RS08550
MMP_RS08555: MMP_RS08555 - hypothetical protein, at 1,604,805 to 1,605,002
_RS08555
MMP_RS08560: MMP_RS08560 - precorrin-4 C(11)-methyltransferase, at 1,604,999 to 1,605,763
_RS08560
MMP_RS08565: MMP_RS08565 - aconitase X catalytic domain-containing protein, at 1,605,793 to 1,606,998
_RS08565
Position (kb)
1604
1605
1606 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 1603.902 kb on - strand at 1604.397 kb on - strand at 1604.400 kb on + strand at 1604.409 kb on + strand at 1604.410 kb on - strand at 1604.411 kb on + strand at 1604.413 kb on + strand at 1604.414 kb on - strand at 1604.421 kb on + strand at 1604.427 kb on - strand at 1604.428 kb on + strand at 1604.428 kb on + strand at 1604.432 kb on - strand at 1604.433 kb on + strand at 1604.433 kb on + strand at 1604.433 kb on + strand at 1604.433 kb on + strand at 1604.436 kb on - strand at 1604.437 kb on + strand at 1604.437 kb on + strand at 1604.842 kb on + strand, within MMP_RS08555 at 1604.926 kb on - strand, within MMP_RS08555 at 1604.928 kb on - strand, within MMP_RS08555 at 1604.929 kb on + strand, within MMP_RS08555 at 1605.073 kb on - strand at 1605.273 kb on - strand at 1605.287 kb on + strand at 1605.287 kb on + strand at 1605.629 kb on + strand at 1605.629 kb on + strand at 1605.631 kb on + strand at 1605.635 kb on - strand at 1605.866 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,603,902 - -1.8 1,604,397 - -1.1 1,604,400 + +0.1 1,604,409 + +0.1 1,604,410 - +1.4 1,604,411 + -0.9 1,604,413 + -3.3 1,604,414 - +2.0 1,604,421 + +0.5 1,604,427 - +0.6 1,604,428 + -0.8 1,604,428 + +0.5 1,604,432 - +1.3 1,604,433 + +0.7 1,604,433 + +0.8 1,604,433 + -0.7 1,604,433 + +0.3 1,604,436 - +0.2 1,604,437 + +0.9 1,604,437 + +0.8 1,604,842 + MMP_RS08555 0.19 +0.1 1,604,926 - MMP_RS08555 0.61 +1.5 1,604,928 - MMP_RS08555 0.62 +0.3 1,604,929 + MMP_RS08555 0.63 +1.2 1,605,073 - -3.1 1,605,273 - -3.1 1,605,287 + -3.2 1,605,287 + -2.4 1,605,629 + -4.6 1,605,629 + -0.3 1,605,631 + -4.8 1,605,635 - -3.2 1,605,866 - -2.8
Or see this region's nucleotide sequence