Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08405 and MMP_RS08410 are separated by 10 nucleotides MMP_RS08410 and MMP_RS08415 are separated by 186 nucleotides MMP_RS08415 and MMP_RS08420 are separated by 9 nucleotides
MMP_RS08405: MMP_RS08405 - MFS transporter, at 1,577,536 to 1,578,906
_RS08405
MMP_RS08410: MMP_RS08410 - DUF169 domain-containing protein, at 1,578,917 to 1,579,609
_RS08410
MMP_RS08415: MMP_RS08415 - radical SAM protein, at 1,579,796 to 1,580,659
_RS08415
MMP_RS08420: MMP_RS08420 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 1,580,669 to 1,581,871
_RS08420
Position (kb)
1579
1580
1581 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1578.904 kb on - strand at 1578.908 kb on - strand at 1579.187 kb on - strand, within MMP_RS08410 at 1579.194 kb on + strand, within MMP_RS08410 at 1579.396 kb on - strand, within MMP_RS08410 at 1579.397 kb on + strand, within MMP_RS08410 at 1579.524 kb on + strand, within MMP_RS08410 at 1579.532 kb on + strand, within MMP_RS08410 at 1579.611 kb on - strand at 1579.611 kb on - strand at 1579.611 kb on - strand at 1579.612 kb on + strand at 1579.675 kb on - strand at 1579.676 kb on + strand at 1579.676 kb on + strand at 1579.720 kb on + strand at 1579.720 kb on + strand at 1579.720 kb on + strand at 1579.723 kb on - strand at 1579.724 kb on + strand at 1579.724 kb on + strand at 1579.725 kb on - strand at 1579.725 kb on - strand at 1579.725 kb on - strand at 1579.727 kb on - strand at 1579.727 kb on - strand at 1579.727 kb on - strand at 1579.728 kb on + strand at 1579.728 kb on + strand at 1579.730 kb on - strand at 1579.730 kb on - strand at 1579.733 kb on - strand at 1579.734 kb on + strand at 1579.736 kb on + strand at 1580.269 kb on + strand, within MMP_RS08415 at 1580.322 kb on + strand, within MMP_RS08415 at 1580.485 kb on + strand, within MMP_RS08415 at 1580.774 kb on + strand at 1580.774 kb on + strand at 1580.800 kb on - strand at 1580.959 kb on + strand at 1581.554 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,578,904 - +0.9 1,578,908 - +0.6 1,579,187 - MMP_RS08410 0.39 +0.4 1,579,194 + MMP_RS08410 0.40 +1.4 1,579,396 - MMP_RS08410 0.69 +0.7 1,579,397 + MMP_RS08410 0.69 +0.5 1,579,524 + MMP_RS08410 0.88 -0.7 1,579,532 + MMP_RS08410 0.89 +0.5 1,579,611 - +0.4 1,579,611 - -0.3 1,579,611 - -0.6 1,579,612 + +0.8 1,579,675 - +0.2 1,579,676 + +0.9 1,579,676 + +0.6 1,579,720 + +0.4 1,579,720 + -0.3 1,579,720 + +0.6 1,579,723 - +0.3 1,579,724 + +1.7 1,579,724 + +1.1 1,579,725 - +0.1 1,579,725 - +0.2 1,579,725 - +0.7 1,579,727 - -0.2 1,579,727 - +0.2 1,579,727 - -0.2 1,579,728 + +0.7 1,579,728 + +0.4 1,579,730 - +0.4 1,579,730 - -0.1 1,579,733 - -0.6 1,579,734 + +1.2 1,579,736 + +0.1 1,580,269 + MMP_RS08415 0.55 +0.0 1,580,322 + MMP_RS08415 0.61 +0.7 1,580,485 + MMP_RS08415 0.80 +1.1 1,580,774 + -2.9 1,580,774 + -1.6 1,580,800 - +0.1 1,580,959 + -3.5 1,581,554 + -3.4
Or see this region's nucleotide sequence