Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08065 and MMP_RS08070 are separated by 86 nucleotides MMP_RS08070 and MMP_RS08075 overlap by 23 nucleotides MMP_RS08075 and MMP_RS08080 are separated by 10 nucleotides MMP_RS08080 and MMP_RS08085 are separated by 8 nucleotides
MMP_RS08065: MMP_RS08065 - tetrahydromethanopterin S-methyltransferase subunit H, at 1,519,462 to 1,520,421
_RS08065
MMP_RS08070: MMP_RS08070 - DUF2096 domain-containing protein, at 1,520,508 to 1,521,041
_RS08070
MMP_RS08075: MMP_RS08075 - DUF749 domain-containing protein, at 1,521,019 to 1,521,327
_RS08075
MMP_RS08080: MMP_RS08080 - YchF/TatD family DNA exonuclease, at 1,521,338 to 1,522,093
_RS08080
MMP_RS08085: MMP_RS08085 - hypothetical protein, at 1,522,102 to 1,523,037
_RS08085
Position (kb)
1521
1522 Strain fitness (log2 ratio)
-1
0
1 at 1520.424 kb on + strand at 1520.488 kb on - strand at 1520.488 kb on - strand at 1520.488 kb on - strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.489 kb on + strand at 1520.510 kb on + strand at 1520.559 kb on - strand at 1520.639 kb on - strand, within MMP_RS08070 at 1520.670 kb on - strand, within MMP_RS08070 at 1520.727 kb on + strand, within MMP_RS08070 at 1520.838 kb on - strand, within MMP_RS08070 at 1520.909 kb on + strand, within MMP_RS08070 at 1520.912 kb on + strand, within MMP_RS08070 at 1520.915 kb on - strand, within MMP_RS08070 at 1521.003 kb on - strand at 1521.004 kb on + strand at 1521.004 kb on + strand at 1521.151 kb on - strand, within MMP_RS08075 at 1521.152 kb on + strand, within MMP_RS08075 at 1521.158 kb on - strand, within MMP_RS08075 at 1521.186 kb on - strand, within MMP_RS08075 at 1521.190 kb on + strand, within MMP_RS08075 at 1521.348 kb on - strand at 1521.350 kb on - strand at 1521.369 kb on - strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.370 kb on + strand at 1521.373 kb on - strand at 1521.493 kb on - strand, within MMP_RS08080 at 1521.493 kb on - strand, within MMP_RS08080 at 1521.564 kb on + strand, within MMP_RS08080 at 1521.572 kb on + strand, within MMP_RS08080 at 1521.577 kb on + strand, within MMP_RS08080 at 1521.755 kb on - strand, within MMP_RS08080 at 1521.849 kb on - strand, within MMP_RS08080 at 1521.868 kb on - strand, within MMP_RS08080 at 1521.881 kb on + strand, within MMP_RS08080 at 1521.909 kb on + strand, within MMP_RS08080 at 1521.977 kb on - strand, within MMP_RS08080 at 1522.010 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.015 kb on - strand, within MMP_RS08080 at 1522.071 kb on - strand at 1522.076 kb on + strand at 1522.086 kb on + strand at 1522.123 kb on - strand at 1522.265 kb on + strand, within MMP_RS08085 at 1522.309 kb on - strand, within MMP_RS08085
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,520,424 + -0.9 1,520,488 - -0.2 1,520,488 - +0.6 1,520,488 - -1.4 1,520,489 + +0.5 1,520,489 + +0.5 1,520,489 + +0.3 1,520,489 + +0.4 1,520,489 + +0.8 1,520,489 + +0.5 1,520,489 + -1.0 1,520,510 + -0.8 1,520,559 - +0.7 1,520,639 - MMP_RS08070 0.25 +1.3 1,520,670 - MMP_RS08070 0.30 +0.2 1,520,727 + MMP_RS08070 0.41 +0.3 1,520,838 - MMP_RS08070 0.62 +1.6 1,520,909 + MMP_RS08070 0.75 -0.0 1,520,912 + MMP_RS08070 0.76 -0.6 1,520,915 - MMP_RS08070 0.76 +0.5 1,521,003 - +0.9 1,521,004 + +0.7 1,521,004 + +0.9 1,521,151 - MMP_RS08075 0.43 -0.4 1,521,152 + MMP_RS08075 0.43 +1.1 1,521,158 - MMP_RS08075 0.45 +0.5 1,521,186 - MMP_RS08075 0.54 +1.6 1,521,190 + MMP_RS08075 0.55 -0.1 1,521,348 - +1.0 1,521,350 - +0.5 1,521,369 - +0.7 1,521,370 + -0.6 1,521,370 + +1.2 1,521,370 + -0.8 1,521,373 - -0.5 1,521,493 - MMP_RS08080 0.21 -0.0 1,521,493 - MMP_RS08080 0.21 +0.3 1,521,564 + MMP_RS08080 0.30 +1.0 1,521,572 + MMP_RS08080 0.31 +1.1 1,521,577 + MMP_RS08080 0.32 -0.4 1,521,755 - MMP_RS08080 0.55 +0.5 1,521,849 - MMP_RS08080 0.68 +0.2 1,521,868 - MMP_RS08080 0.70 +0.8 1,521,881 + MMP_RS08080 0.72 -1.6 1,521,909 + MMP_RS08080 0.76 +0.4 1,521,977 - MMP_RS08080 0.85 +0.5 1,522,010 - MMP_RS08080 0.89 -0.5 1,522,015 - MMP_RS08080 0.90 -0.8 1,522,015 - MMP_RS08080 0.90 +0.7 1,522,071 - +1.2 1,522,076 + +0.4 1,522,086 + +0.3 1,522,123 - +0.2 1,522,265 + MMP_RS08085 0.17 +0.4 1,522,309 - MMP_RS08085 0.22 +0.4
Or see this region's nucleotide sequence