Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07950 and MMP_RS07955 are separated by 86 nucleotides MMP_RS07955 and MMP_RS07960 are separated by 77 nucleotides MMP_RS07960 and MMP_RS07965 are separated by 21 nucleotides MMP_RS07965 and MMP_RS07970 overlap by 4 nucleotides
MMP_RS07950: MMP_RS07950 - 30S ribosomal protein S19, at 1,501,753 to 1,502,238
_RS07950
MMP_RS07955: MMP_RS07955 - FumA C-terminus/TtdB family hydratase beta subunit, at 1,502,325 to 1,502,897
_RS07955
MMP_RS07960: MMP_RS07960 - sugar phosphate isomerase/epimerase, at 1,502,975 to 1,503,781
_RS07960
MMP_RS07965: MMP_RS07965 - NADPH-dependent F420 reductase, at 1,503,803 to 1,504,474
_RS07965
MMP_RS07970: MMP_RS07970 - hypothetical protein, at 1,504,471 to 1,504,692
_RS07970
Position (kb)
1502
1503
1504 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1502.379 kb on + strand at 1502.385 kb on - strand, within MMP_RS07955 at 1502.386 kb on + strand, within MMP_RS07955 at 1502.386 kb on + strand, within MMP_RS07955 at 1502.390 kb on - strand, within MMP_RS07955 at 1502.483 kb on + strand, within MMP_RS07955 at 1502.622 kb on + strand, within MMP_RS07955 at 1502.635 kb on + strand, within MMP_RS07955 at 1502.738 kb on + strand, within MMP_RS07955 at 1502.761 kb on + strand, within MMP_RS07955 at 1502.793 kb on + strand, within MMP_RS07955 at 1502.793 kb on + strand, within MMP_RS07955 at 1502.836 kb on + strand, within MMP_RS07955 at 1502.925 kb on - strand at 1502.926 kb on + strand at 1502.946 kb on + strand at 1502.951 kb on - strand at 1502.951 kb on - strand at 1503.294 kb on - strand, within MMP_RS07960 at 1503.327 kb on - strand, within MMP_RS07960 at 1503.360 kb on - strand, within MMP_RS07960 at 1503.361 kb on + strand, within MMP_RS07960 at 1503.460 kb on - strand, within MMP_RS07960 at 1503.494 kb on + strand, within MMP_RS07960 at 1503.680 kb on + strand, within MMP_RS07960 at 1503.683 kb on + strand, within MMP_RS07960 at 1503.686 kb on - strand, within MMP_RS07960 at 1503.686 kb on - strand, within MMP_RS07960 at 1503.687 kb on + strand, within MMP_RS07960 at 1503.759 kb on + strand at 1504.038 kb on + strand at 1504.092 kb on - strand at 1504.465 kb on + strand at 1504.472 kb on - strand at 1504.548 kb on - strand, within MMP_RS07970 at 1504.577 kb on + strand, within MMP_RS07970 at 1504.683 kb on - strand at 1504.724 kb on + strand at 1504.755 kb on + strand at 1504.781 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,502,379 + -0.2 1,502,385 - MMP_RS07955 0.10 -2.3 1,502,386 + MMP_RS07955 0.11 -0.0 1,502,386 + MMP_RS07955 0.11 -2.0 1,502,390 - MMP_RS07955 0.11 -2.3 1,502,483 + MMP_RS07955 0.28 -1.2 1,502,622 + MMP_RS07955 0.52 -2.9 1,502,635 + MMP_RS07955 0.54 -2.4 1,502,738 + MMP_RS07955 0.72 -3.6 1,502,761 + MMP_RS07955 0.76 -0.6 1,502,793 + MMP_RS07955 0.82 -4.7 1,502,793 + MMP_RS07955 0.82 -3.2 1,502,836 + MMP_RS07955 0.89 -2.5 1,502,925 - +0.4 1,502,926 + -2.5 1,502,946 + +0.3 1,502,951 - +2.0 1,502,951 - +1.4 1,503,294 - MMP_RS07960 0.40 +0.1 1,503,327 - MMP_RS07960 0.44 +0.8 1,503,360 - MMP_RS07960 0.48 -0.1 1,503,361 + MMP_RS07960 0.48 +0.8 1,503,460 - MMP_RS07960 0.60 +1.0 1,503,494 + MMP_RS07960 0.64 +0.7 1,503,680 + MMP_RS07960 0.87 +2.4 1,503,683 + MMP_RS07960 0.88 +0.1 1,503,686 - MMP_RS07960 0.88 +0.3 1,503,686 - MMP_RS07960 0.88 -0.1 1,503,687 + MMP_RS07960 0.88 +0.2 1,503,759 + -0.0 1,504,038 + -2.5 1,504,092 - -2.2 1,504,465 + +0.5 1,504,472 - +0.1 1,504,548 - MMP_RS07970 0.35 +0.4 1,504,577 + MMP_RS07970 0.48 +0.2 1,504,683 - -0.4 1,504,724 + +0.5 1,504,755 + +0.5 1,504,781 + +0.3
Or see this region's nucleotide sequence