Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07845 and MMP_RS07850 are separated by 110 nucleotides MMP_RS07850 and MMP_RS07855 overlap by 4 nucleotides MMP_RS07855 and MMP_RS07860 overlap by 4 nucleotides
MMP_RS07845: MMP_RS07845 - LL-diaminopimelate aminotransferase, at 1,486,064 to 1,487,314
_RS07845
MMP_RS07850: MMP_RS07850 - prephenate dehydratase, at 1,487,425 to 1,488,231
_RS07850
MMP_RS07855: MMP_RS07855 - class I SAM-dependent methyltransferase, at 1,488,228 to 1,488,938
_RS07855
MMP_RS07860: MMP_RS07860 - hypothetical protein, at 1,488,935 to 1,489,225
_RS07860
Position (kb) 1487
1488
1489 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 1486.477 kb on + strand, within MMP_RS07845 at 1486.557 kb on - strand, within MMP_RS07845 at 1486.755 kb on - strand, within MMP_RS07845 at 1487.346 kb on - strand at 1487.366 kb on + strand at 1487.387 kb on - strand at 1487.388 kb on + strand at 1487.455 kb on - strand at 1487.507 kb on - strand, within MMP_RS07850 at 1487.517 kb on + strand, within MMP_RS07850 at 1487.707 kb on + strand, within MMP_RS07850 at 1488.315 kb on - strand, within MMP_RS07855 at 1488.381 kb on - strand, within MMP_RS07855 at 1488.382 kb on + strand, within MMP_RS07855 at 1488.383 kb on - strand, within MMP_RS07855 at 1488.383 kb on - strand, within MMP_RS07855 at 1488.386 kb on + strand, within MMP_RS07855 at 1488.703 kb on - strand, within MMP_RS07855 at 1488.703 kb on - strand, within MMP_RS07855 at 1488.703 kb on - strand, within MMP_RS07855 at 1488.704 kb on + strand, within MMP_RS07855 at 1488.704 kb on + strand, within MMP_RS07855 at 1488.704 kb on + strand, within MMP_RS07855 at 1488.705 kb on - strand, within MMP_RS07855 at 1488.705 kb on - strand, within MMP_RS07855 at 1488.706 kb on + strand, within MMP_RS07855 at 1488.706 kb on + strand, within MMP_RS07855 at 1488.733 kb on - strand, within MMP_RS07855 at 1488.734 kb on + strand, within MMP_RS07855 at 1488.913 kb on + strand at 1488.953 kb on + strand at 1488.953 kb on + strand at 1489.071 kb on - strand, within MMP_RS07860 at 1489.198 kb on + strand at 1489.227 kb on - strand at 1489.228 kb on + strand at 1489.228 kb on + strand at 1489.228 kb on + strand at 1489.229 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,486,477 + MMP_RS07845 0.33 -4.9 1,486,557 - MMP_RS07845 0.39 -3.9 1,486,755 - MMP_RS07845 0.55 -4.9 1,487,346 - -3.4 1,487,366 + +0.2 1,487,387 - -0.4 1,487,388 + -0.6 1,487,455 - -0.4 1,487,507 - MMP_RS07850 0.10 +0.2 1,487,517 + MMP_RS07850 0.11 +0.0 1,487,707 + MMP_RS07850 0.35 +1.1 1,488,315 - MMP_RS07855 0.12 -0.4 1,488,381 - MMP_RS07855 0.22 +0.6 1,488,382 + MMP_RS07855 0.22 -0.8 1,488,383 - MMP_RS07855 0.22 -0.1 1,488,383 - MMP_RS07855 0.22 +0.3 1,488,386 + MMP_RS07855 0.22 -0.3 1,488,703 - MMP_RS07855 0.67 +1.2 1,488,703 - MMP_RS07855 0.67 +2.3 1,488,703 - MMP_RS07855 0.67 +0.4 1,488,704 + MMP_RS07855 0.67 +1.4 1,488,704 + MMP_RS07855 0.67 -1.1 1,488,704 + MMP_RS07855 0.67 -1.7 1,488,705 - MMP_RS07855 0.67 -0.1 1,488,705 - MMP_RS07855 0.67 -1.2 1,488,706 + MMP_RS07855 0.67 +1.5 1,488,706 + MMP_RS07855 0.67 +0.9 1,488,733 - MMP_RS07855 0.71 -0.6 1,488,734 + MMP_RS07855 0.71 +0.4 1,488,913 + +1.0 1,488,953 + -0.1 1,488,953 + -1.3 1,489,071 - MMP_RS07860 0.47 -0.9 1,489,198 + +1.3 1,489,227 - -0.3 1,489,228 + +0.9 1,489,228 + +0.7 1,489,228 + -0.4 1,489,229 - -0.9
Or see this region's nucleotide sequence