Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07790 and MMP_RS07795 are separated by 18 nucleotides MMP_RS07795 and MMP_RS07800 are separated by 132 nucleotides MMP_RS07800 and MMP_RS07805 are separated by 14 nucleotides MMP_RS07805 and MMP_RS07810 are separated by 166 nucleotides
MMP_RS07790: MMP_RS07790 - segregation/condensation protein A, at 1,476,368 to 1,477,036
_RS07790
MMP_RS07795: MMP_RS07795 - site-2 protease family protein, at 1,477,055 to 1,477,657
_RS07795
MMP_RS07800: MMP_RS07800 - ATP-binding cassette domain-containing protein, at 1,477,790 to 1,478,728
_RS07800
MMP_RS07805: MMP_RS07805 - ABC transporter permease, at 1,478,743 to 1,479,510
_RS07805
MMP_RS07810: MMP_RS07810 - hydrogenase nickel incorporation protein HypB, at 1,479,677 to 1,480,351
_RS07810
Position (kb)
1477
1478
1479 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1476.927 kb on + strand, within MMP_RS07790 at 1476.950 kb on - strand, within MMP_RS07790 at 1476.950 kb on - strand, within MMP_RS07790 at 1477.148 kb on - strand, within MMP_RS07795 at 1477.180 kb on - strand, within MMP_RS07795 at 1477.184 kb on - strand, within MMP_RS07795 at 1477.208 kb on + strand, within MMP_RS07795 at 1477.216 kb on + strand, within MMP_RS07795 at 1477.216 kb on + strand, within MMP_RS07795 at 1477.227 kb on - strand, within MMP_RS07795 at 1477.231 kb on + strand, within MMP_RS07795 at 1477.231 kb on + strand, within MMP_RS07795 at 1477.342 kb on - strand, within MMP_RS07795 at 1477.343 kb on + strand, within MMP_RS07795 at 1477.344 kb on - strand, within MMP_RS07795 at 1477.344 kb on - strand, within MMP_RS07795 at 1477.344 kb on - strand, within MMP_RS07795 at 1477.345 kb on + strand, within MMP_RS07795 at 1477.345 kb on + strand, within MMP_RS07795 at 1477.346 kb on - strand, within MMP_RS07795 at 1477.543 kb on - strand, within MMP_RS07795 at 1477.574 kb on - strand, within MMP_RS07795 at 1477.575 kb on + strand, within MMP_RS07795 at 1477.579 kb on - strand, within MMP_RS07795 at 1477.579 kb on - strand, within MMP_RS07795 at 1477.580 kb on + strand, within MMP_RS07795 at 1477.627 kb on - strand at 1477.675 kb on + strand at 1477.714 kb on - strand at 1477.714 kb on - strand at 1477.723 kb on - strand at 1477.724 kb on + strand at 1477.724 kb on + strand at 1477.771 kb on + strand at 1477.786 kb on - strand at 1477.786 kb on - strand at 1477.819 kb on - strand at 1477.823 kb on + strand at 1478.178 kb on + strand, within MMP_RS07800 at 1478.237 kb on - strand, within MMP_RS07800 at 1478.237 kb on - strand, within MMP_RS07800 at 1478.339 kb on - strand, within MMP_RS07800 at 1478.339 kb on - strand, within MMP_RS07800 at 1478.340 kb on + strand, within MMP_RS07800 at 1478.363 kb on - strand, within MMP_RS07800 at 1478.364 kb on + strand, within MMP_RS07800 at 1478.364 kb on + strand, within MMP_RS07800 at 1478.366 kb on + strand, within MMP_RS07800 at 1478.366 kb on + strand, within MMP_RS07800 at 1478.543 kb on + strand, within MMP_RS07800 at 1478.594 kb on - strand, within MMP_RS07800 at 1478.704 kb on - strand at 1478.722 kb on - strand at 1478.722 kb on - strand at 1478.722 kb on - strand at 1478.732 kb on - strand at 1478.734 kb on - strand at 1478.745 kb on + strand at 1478.754 kb on + strand at 1478.754 kb on - strand at 1478.755 kb on + strand at 1478.757 kb on + strand at 1478.762 kb on + strand at 1478.791 kb on + strand at 1478.791 kb on + strand at 1478.809 kb on + strand at 1478.888 kb on - strand, within MMP_RS07805 at 1478.889 kb on + strand, within MMP_RS07805 at 1479.086 kb on - strand, within MMP_RS07805 at 1479.110 kb on + strand, within MMP_RS07805 at 1479.207 kb on - strand, within MMP_RS07805 at 1479.208 kb on + strand, within MMP_RS07805 at 1479.208 kb on + strand, within MMP_RS07805 at 1479.212 kb on + strand, within MMP_RS07805 at 1479.246 kb on - strand, within MMP_RS07805 at 1479.297 kb on + strand, within MMP_RS07805 at 1479.358 kb on - strand, within MMP_RS07805 at 1479.443 kb on + strand at 1479.534 kb on + strand at 1479.601 kb on - strand at 1479.605 kb on + strand at 1479.621 kb on - strand at 1479.640 kb on - strand at 1479.640 kb on - strand at 1479.641 kb on + strand at 1479.641 kb on + strand at 1479.641 kb on + strand at 1479.651 kb on - strand at 1479.652 kb on + strand at 1479.652 kb on + strand at 1479.714 kb on - strand at 1479.715 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,476,927 + MMP_RS07790 0.84 +0.0 1,476,950 - MMP_RS07790 0.87 +0.6 1,476,950 - MMP_RS07790 0.87 +0.4 1,477,148 - MMP_RS07795 0.15 +0.5 1,477,180 - MMP_RS07795 0.21 +0.8 1,477,184 - MMP_RS07795 0.21 +1.0 1,477,208 + MMP_RS07795 0.25 +0.1 1,477,216 + MMP_RS07795 0.27 +0.1 1,477,216 + MMP_RS07795 0.27 +0.0 1,477,227 - MMP_RS07795 0.29 +0.7 1,477,231 + MMP_RS07795 0.29 -0.1 1,477,231 + MMP_RS07795 0.29 -0.9 1,477,342 - MMP_RS07795 0.48 +1.0 1,477,343 + MMP_RS07795 0.48 +0.2 1,477,344 - MMP_RS07795 0.48 +0.8 1,477,344 - MMP_RS07795 0.48 -0.7 1,477,344 - MMP_RS07795 0.48 +1.2 1,477,345 + MMP_RS07795 0.48 +0.0 1,477,345 + MMP_RS07795 0.48 +0.3 1,477,346 - MMP_RS07795 0.48 -2.7 1,477,543 - MMP_RS07795 0.81 -0.1 1,477,574 - MMP_RS07795 0.86 +0.4 1,477,575 + MMP_RS07795 0.86 -0.5 1,477,579 - MMP_RS07795 0.87 +0.9 1,477,579 - MMP_RS07795 0.87 -0.9 1,477,580 + MMP_RS07795 0.87 +0.3 1,477,627 - -0.6 1,477,675 + +1.1 1,477,714 - +1.6 1,477,714 - -0.9 1,477,723 - +0.9 1,477,724 + +0.2 1,477,724 + +0.6 1,477,771 + +0.3 1,477,786 - +0.2 1,477,786 - -1.9 1,477,819 - +0.6 1,477,823 + +0.9 1,478,178 + MMP_RS07800 0.41 +0.3 1,478,237 - MMP_RS07800 0.48 +1.0 1,478,237 - MMP_RS07800 0.48 +1.7 1,478,339 - MMP_RS07800 0.58 +1.2 1,478,339 - MMP_RS07800 0.58 +1.1 1,478,340 + MMP_RS07800 0.59 +0.7 1,478,363 - MMP_RS07800 0.61 +0.3 1,478,364 + MMP_RS07800 0.61 +0.1 1,478,364 + MMP_RS07800 0.61 +0.6 1,478,366 + MMP_RS07800 0.61 +1.1 1,478,366 + MMP_RS07800 0.61 +0.6 1,478,543 + MMP_RS07800 0.80 -0.5 1,478,594 - MMP_RS07800 0.86 -1.0 1,478,704 - +1.2 1,478,722 - +1.5 1,478,722 - +0.1 1,478,722 - +0.1 1,478,732 - +0.8 1,478,734 - -0.1 1,478,745 + -0.5 1,478,754 + -0.4 1,478,754 - +1.1 1,478,755 + +0.4 1,478,757 + +1.0 1,478,762 + +0.6 1,478,791 + +0.9 1,478,791 + -0.0 1,478,809 + +0.4 1,478,888 - MMP_RS07805 0.19 +0.8 1,478,889 + MMP_RS07805 0.19 +1.2 1,479,086 - MMP_RS07805 0.45 +0.5 1,479,110 + MMP_RS07805 0.48 +1.0 1,479,207 - MMP_RS07805 0.60 -0.6 1,479,208 + MMP_RS07805 0.61 +0.5 1,479,208 + MMP_RS07805 0.61 +0.4 1,479,212 + MMP_RS07805 0.61 +0.6 1,479,246 - MMP_RS07805 0.65 +0.5 1,479,297 + MMP_RS07805 0.72 +0.1 1,479,358 - MMP_RS07805 0.80 -0.1 1,479,443 + +0.3 1,479,534 + -0.9 1,479,601 - -0.0 1,479,605 + +0.6 1,479,621 - -1.0 1,479,640 - -4.1 1,479,640 - -2.2 1,479,641 + -0.2 1,479,641 + -2.9 1,479,641 + +0.0 1,479,651 - -4.3 1,479,652 + -2.5 1,479,652 + +0.7 1,479,714 - -4.4 1,479,715 + -3.7
Or see this region's nucleotide sequence