Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07760 and MMP_RS07765 are separated by 177 nucleotides MMP_RS07765 and MMP_RS07770 are separated by 222 nucleotides
MMP_RS07760: MMP_RS07760 - Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA, at 1,467,465 to 1,468,760
_RS07760
MMP_RS07765: MMP_RS07765 - sodium:alanine symporter family protein, at 1,468,938 to 1,470,299
_RS07765
MMP_RS07770: MMP_RS07770 - alanine racemase, at 1,470,522 to 1,471,643
_RS07770
Position (kb)
1468
1469
1470
1471 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1468.156 kb on + strand at 1468.242 kb on - strand at 1468.802 kb on - strand at 1468.829 kb on + strand at 1468.829 kb on + strand at 1468.845 kb on + strand at 1468.924 kb on + strand at 1468.965 kb on - strand at 1469.132 kb on + strand, within MMP_RS07765 at 1469.361 kb on + strand, within MMP_RS07765 at 1469.364 kb on - strand, within MMP_RS07765 at 1469.365 kb on + strand, within MMP_RS07765 at 1469.566 kb on + strand, within MMP_RS07765 at 1469.666 kb on - strand, within MMP_RS07765 at 1469.684 kb on - strand, within MMP_RS07765 at 1469.699 kb on - strand, within MMP_RS07765 at 1469.740 kb on + strand, within MMP_RS07765 at 1469.945 kb on - strand, within MMP_RS07765 at 1470.037 kb on - strand, within MMP_RS07765 at 1470.169 kb on - strand at 1470.198 kb on - strand at 1470.199 kb on + strand at 1470.247 kb on - strand at 1470.247 kb on - strand at 1470.247 kb on - strand at 1470.248 kb on + strand at 1470.248 kb on + strand at 1470.248 kb on + strand at 1470.270 kb on + strand at 1470.278 kb on - strand at 1470.371 kb on - strand at 1470.372 kb on + strand at 1470.372 kb on + strand at 1470.388 kb on + strand at 1470.388 kb on + strand at 1470.444 kb on - strand at 1470.445 kb on + strand at 1470.453 kb on - strand at 1470.458 kb on - strand at 1470.461 kb on - strand at 1470.461 kb on - strand at 1470.524 kb on + strand at 1470.528 kb on - strand at 1470.528 kb on - strand at 1470.528 kb on - strand at 1470.532 kb on - strand at 1470.532 kb on - strand at 1470.532 kb on - strand at 1470.532 kb on - strand at 1470.535 kb on + strand at 1470.535 kb on + strand at 1470.535 kb on + strand at 1470.574 kb on - strand at 1470.579 kb on - strand at 1470.643 kb on + strand, within MMP_RS07770 at 1470.643 kb on + strand, within MMP_RS07770 at 1470.683 kb on - strand, within MMP_RS07770 at 1470.853 kb on + strand, within MMP_RS07770 at 1470.853 kb on + strand, within MMP_RS07770 at 1470.895 kb on + strand, within MMP_RS07770 at 1470.966 kb on - strand, within MMP_RS07770 at 1470.967 kb on + strand, within MMP_RS07770 at 1470.984 kb on - strand, within MMP_RS07770 at 1470.989 kb on - strand, within MMP_RS07770 at 1471.117 kb on - strand, within MMP_RS07770
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,468,156 + -3.3 1,468,242 - -2.7 1,468,802 - -4.3 1,468,829 + -0.8 1,468,829 + +0.6 1,468,845 + -0.7 1,468,924 + -0.0 1,468,965 - +1.7 1,469,132 + MMP_RS07765 0.14 +1.5 1,469,361 + MMP_RS07765 0.31 +1.6 1,469,364 - MMP_RS07765 0.31 +1.1 1,469,365 + MMP_RS07765 0.31 +0.7 1,469,566 + MMP_RS07765 0.46 +1.7 1,469,666 - MMP_RS07765 0.53 +2.0 1,469,684 - MMP_RS07765 0.55 +1.3 1,469,699 - MMP_RS07765 0.56 +1.6 1,469,740 + MMP_RS07765 0.59 +1.2 1,469,945 - MMP_RS07765 0.74 +2.3 1,470,037 - MMP_RS07765 0.81 +1.8 1,470,169 - -0.3 1,470,198 - +1.0 1,470,199 + +1.1 1,470,247 - +1.7 1,470,247 - +2.0 1,470,247 - +1.8 1,470,248 + +1.1 1,470,248 + +1.5 1,470,248 + +0.5 1,470,270 + +0.6 1,470,278 - +2.2 1,470,371 - +1.9 1,470,372 + +2.2 1,470,372 + +2.4 1,470,388 + +2.3 1,470,388 + +1.8 1,470,444 - -0.1 1,470,445 + +0.8 1,470,453 - -0.3 1,470,458 - +0.4 1,470,461 - -0.1 1,470,461 - +0.6 1,470,524 + +0.2 1,470,528 - +0.8 1,470,528 - +0.3 1,470,528 - -0.1 1,470,532 - +0.5 1,470,532 - -0.3 1,470,532 - +0.6 1,470,532 - -0.5 1,470,535 + +1.1 1,470,535 + -0.9 1,470,535 + +0.7 1,470,574 - +0.7 1,470,579 - -0.1 1,470,643 + MMP_RS07770 0.11 +1.2 1,470,643 + MMP_RS07770 0.11 +1.1 1,470,683 - MMP_RS07770 0.14 +1.6 1,470,853 + MMP_RS07770 0.30 +1.2 1,470,853 + MMP_RS07770 0.30 +0.1 1,470,895 + MMP_RS07770 0.33 -1.7 1,470,966 - MMP_RS07770 0.40 +0.5 1,470,967 + MMP_RS07770 0.40 +0.7 1,470,984 - MMP_RS07770 0.41 +0.1 1,470,989 - MMP_RS07770 0.42 +0.7 1,471,117 - MMP_RS07770 0.53 +0.3
Or see this region's nucleotide sequence