Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS07345 and MMP_RS07350 are separated by 72 nucleotides MMP_RS07350 and MMP_RS07355 are separated by 54 nucleotides MMP_RS07355 and MMP_RS07360 are separated by 71 nucleotides
MMP_RS07345: MMP_RS07345 - glycine synthesis protein GlyXL (from data) , at 1,399,464 to 1,400,840
_RS07345
MMP_RS07350: MMP_RS07350 - preprotein translocase subunit Sec61beta, at 1,400,913 to 1,401,074
_RS07350
MMP_RS07355: MMP_RS07355 - transcription factor S, at 1,401,129 to 1,401,446
_RS07355
MMP_RS07360: MMP_RS07360 - TrkH family potassium uptake protein, at 1,401,518 to 1,402,954
_RS07360
Position (kb)
1400
1401
1402 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1399.959 kb on + strand, within MMP_RS07345 at 1399.965 kb on - strand, within MMP_RS07345 at 1399.995 kb on + strand, within MMP_RS07345 at 1400.292 kb on + strand, within MMP_RS07345 at 1400.337 kb on + strand, within MMP_RS07345 at 1400.451 kb on + strand, within MMP_RS07345 at 1400.465 kb on - strand, within MMP_RS07345 at 1400.466 kb on + strand, within MMP_RS07345 at 1400.673 kb on + strand, within MMP_RS07345 at 1400.889 kb on + strand at 1400.890 kb on - strand at 1400.891 kb on + strand at 1400.965 kb on + strand, within MMP_RS07350 at 1400.965 kb on + strand, within MMP_RS07350 at 1400.989 kb on - strand, within MMP_RS07350 at 1401.112 kb on + strand at 1401.127 kb on + strand at 1401.131 kb on + strand at 1401.131 kb on + strand at 1401.137 kb on + strand at 1401.150 kb on + strand at 1401.255 kb on - strand, within MMP_RS07355 at 1401.417 kb on + strand at 1401.479 kb on + strand at 1401.481 kb on + strand at 1401.481 kb on + strand at 1401.512 kb on - strand at 1401.574 kb on + strand at 1401.796 kb on + strand at 1401.811 kb on - strand at 1402.015 kb on - strand at 1402.032 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,399,959 + MMP_RS07345 0.36 -4.9 1,399,965 - MMP_RS07345 0.36 -5.9 1,399,995 + MMP_RS07345 0.39 -3.3 1,400,292 + MMP_RS07345 0.60 -5.4 1,400,337 + MMP_RS07345 0.63 -4.6 1,400,451 + MMP_RS07345 0.72 -5.6 1,400,465 - MMP_RS07345 0.73 -4.7 1,400,466 + MMP_RS07345 0.73 -4.1 1,400,673 + MMP_RS07345 0.88 -5.4 1,400,889 + -0.1 1,400,890 - +0.6 1,400,891 + -1.5 1,400,965 + MMP_RS07350 0.32 +0.2 1,400,965 + MMP_RS07350 0.32 +0.8 1,400,989 - MMP_RS07350 0.47 +0.6 1,401,112 + +0.5 1,401,127 + -0.6 1,401,131 + +1.1 1,401,131 + +0.8 1,401,137 + +0.0 1,401,150 + -3.1 1,401,255 - MMP_RS07355 0.40 -2.0 1,401,417 + -2.8 1,401,479 + -2.1 1,401,481 + -3.7 1,401,481 + -1.0 1,401,512 - -2.1 1,401,574 + -5.6 1,401,796 + -0.6 1,401,811 - -0.9 1,402,015 - -1.1 1,402,032 + -1.2
Or see this region's nucleotide sequence