Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06575 and MMP_RS06580 are separated by 100 nucleotides MMP_RS06580 and MMP_RS06585 are separated by 71 nucleotides MMP_RS06585 and MMP_RS06590 are separated by 71 nucleotides MMP_RS06590 and MMP_RS06595 are separated by 43 nucleotides MMP_RS06595 and MMP_RS06600 are separated by 37 nucleotides MMP_RS06600 and MMP_RS06605 are separated by 112 nucleotides
MMP_RS06575: MMP_RS06575 - fluoride efflux transporter CrcB, at 1,263,122 to 1,263,490
_RS06575
MMP_RS06580: MMP_RS06580 - tRNA-Arg, at 1,263,591 to 1,263,665
_RS06580
MMP_RS06585: MMP_RS06585 - DMT family protein, at 1,263,737 to 1,264,075
_RS06585
MMP_RS06590: MMP_RS06590 - GNAT family N-acetyltransferase, at 1,264,147 to 1,264,653
_RS06590
MMP_RS06595: MMP_RS06595 - flagellar hook-basal body complex protein FliE, at 1,264,697 to 1,265,248
_RS06595
MMP_RS06600: MMP_RS06600 - class III signal peptide-containing protein, at 1,265,286 to 1,265,489
_RS06600
MMP_RS06605: MMP_RS06605 - DUF5814 domain-containing protein, at 1,265,602 to 1,268,037
_RS06605
Position (kb)
1264
1265 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1263.216 kb on - strand, within MMP_RS06575 at 1263.339 kb on - strand, within MMP_RS06575 at 1263.380 kb on - strand, within MMP_RS06575 at 1263.382 kb on - strand, within MMP_RS06575 at 1263.382 kb on - strand, within MMP_RS06575 at 1263.503 kb on - strand at 1263.542 kb on + strand at 1263.543 kb on - strand at 1263.545 kb on - strand at 1263.554 kb on + strand at 1263.739 kb on + strand at 1263.997 kb on + strand, within MMP_RS06585 at 1264.065 kb on - strand at 1264.103 kb on + strand at 1264.105 kb on + strand at 1264.106 kb on - strand at 1264.121 kb on - strand at 1264.153 kb on + strand at 1264.155 kb on - strand at 1264.404 kb on - strand, within MMP_RS06590 at 1264.417 kb on - strand, within MMP_RS06590 at 1264.417 kb on - strand, within MMP_RS06590 at 1264.591 kb on - strand, within MMP_RS06590 at 1264.692 kb on + strand at 1264.703 kb on - strand at 1264.703 kb on - strand at 1265.287 kb on - strand at 1265.310 kb on - strand, within MMP_RS06600 at 1265.311 kb on + strand, within MMP_RS06600 at 1265.510 kb on - strand at 1265.511 kb on + strand at 1265.521 kb on - strand at 1265.526 kb on + strand at 1265.526 kb on + strand at 1265.544 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,263,216 - MMP_RS06575 0.25 +1.1 1,263,339 - MMP_RS06575 0.59 -0.5 1,263,380 - MMP_RS06575 0.70 +1.0 1,263,382 - MMP_RS06575 0.70 -0.3 1,263,382 - MMP_RS06575 0.70 -0.1 1,263,503 - +0.4 1,263,542 + +0.4 1,263,543 - -4.6 1,263,545 - -2.7 1,263,554 + -2.8 1,263,739 + +1.1 1,263,997 + MMP_RS06585 0.77 +0.3 1,264,065 - +0.8 1,264,103 + -0.4 1,264,105 + +0.1 1,264,106 - +0.2 1,264,121 - -0.0 1,264,153 + +0.3 1,264,155 - -1.2 1,264,404 - MMP_RS06590 0.51 +0.2 1,264,417 - MMP_RS06590 0.53 -0.4 1,264,417 - MMP_RS06590 0.53 +1.1 1,264,591 - MMP_RS06590 0.88 +0.5 1,264,692 + +0.2 1,264,703 - -1.1 1,264,703 - +1.1 1,265,287 - -1.5 1,265,310 - MMP_RS06600 0.12 +1.0 1,265,311 + MMP_RS06600 0.12 -1.4 1,265,510 - -0.7 1,265,511 + -1.7 1,265,521 - +0.5 1,265,526 + -3.1 1,265,526 + -0.1 1,265,544 - +0.2
Or see this region's nucleotide sequence