Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06565 and MMP_RS06570 are separated by 22 nucleotides MMP_RS06570 and MMP_RS06575 are separated by 4 nucleotides MMP_RS06575 and MMP_RS06580 are separated by 100 nucleotides MMP_RS06580 and MMP_RS06585 are separated by 71 nucleotides
MMP_RS06565: MMP_RS06565 - fumarate reductase subunit A, at 1,261,146 to 1,262,765
_RS06565
MMP_RS06570: MMP_RS06570 - DUF190 domain-containing protein, at 1,262,788 to 1,263,117
_RS06570
MMP_RS06575: MMP_RS06575 - fluoride efflux transporter CrcB, at 1,263,122 to 1,263,490
_RS06575
MMP_RS06580: MMP_RS06580 - tRNA-Arg, at 1,263,591 to 1,263,665
_RS06580
MMP_RS06585: MMP_RS06585 - DMT family protein, at 1,263,737 to 1,264,075
_RS06585
Position (kb) 1262
1263
1264 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1262.157 kb on + strand at 1262.752 kb on + strand at 1262.830 kb on - strand, within MMP_RS06570 at 1262.872 kb on - strand, within MMP_RS06570 at 1262.874 kb on - strand, within MMP_RS06570 at 1262.894 kb on - strand, within MMP_RS06570 at 1263.022 kb on - strand, within MMP_RS06570 at 1263.035 kb on + strand, within MMP_RS06570 at 1263.216 kb on - strand, within MMP_RS06575 at 1263.339 kb on - strand, within MMP_RS06575 at 1263.380 kb on - strand, within MMP_RS06575 at 1263.382 kb on - strand, within MMP_RS06575 at 1263.382 kb on - strand, within MMP_RS06575 at 1263.503 kb on - strand at 1263.542 kb on + strand at 1263.543 kb on - strand at 1263.545 kb on - strand at 1263.554 kb on + strand at 1263.739 kb on + strand at 1263.997 kb on + strand, within MMP_RS06585 at 1264.065 kb on - strand at 1264.103 kb on + strand at 1264.105 kb on + strand at 1264.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,262,157 + -2.0 1,262,752 + -2.4 1,262,830 - MMP_RS06570 0.13 +1.4 1,262,872 - MMP_RS06570 0.25 -0.0 1,262,874 - MMP_RS06570 0.26 +0.0 1,262,894 - MMP_RS06570 0.32 +0.4 1,263,022 - MMP_RS06570 0.71 +1.2 1,263,035 + MMP_RS06570 0.75 +0.5 1,263,216 - MMP_RS06575 0.25 +1.1 1,263,339 - MMP_RS06575 0.59 -0.5 1,263,380 - MMP_RS06575 0.70 +1.0 1,263,382 - MMP_RS06575 0.70 -0.3 1,263,382 - MMP_RS06575 0.70 -0.1 1,263,503 - +0.4 1,263,542 + +0.4 1,263,543 - -4.6 1,263,545 - -2.7 1,263,554 + -2.8 1,263,739 + +1.1 1,263,997 + MMP_RS06585 0.77 +0.3 1,264,065 - +0.8 1,264,103 + -0.4 1,264,105 + +0.1 1,264,106 - +0.2
Or see this region's nucleotide sequence