Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06535 and MMP_RS06540 are separated by 1 nucleotides MMP_RS06540 and MMP_RS06545 are separated by 10 nucleotides MMP_RS06545 and MMP_RS06550 are separated by 83 nucleotides
MMP_RS06535: MMP_RS06535 - 2-oxoacid:acceptor oxidoreductase family protein, at 1,255,312 to 1,256,751
_RS06535
MMP_RS06540: MMP_RS06540 - 3-methyl-2-oxobutanoate dehydrogenase subunit VorB, at 1,256,753 to 1,257,808
_RS06540
MMP_RS06545: MMP_RS06545 - ferredoxin family protein, at 1,257,819 to 1,258,058
_RS06545
MMP_RS06550: MMP_RS06550 - AMP-binding protein, at 1,258,142 to 1,259,815
_RS06550
Position (kb)
1256
1257
1258 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1255.766 kb on - strand, within MMP_RS06535 at 1255.766 kb on - strand, within MMP_RS06535 at 1255.912 kb on - strand, within MMP_RS06535 at 1256.538 kb on + strand, within MMP_RS06535 at 1256.538 kb on + strand, within MMP_RS06535 at 1256.584 kb on + strand, within MMP_RS06535 at 1257.081 kb on + strand, within MMP_RS06540 at 1257.087 kb on - strand, within MMP_RS06540 at 1257.685 kb on + strand, within MMP_RS06540 at 1257.705 kb on + strand at 1257.738 kb on - strand at 1257.756 kb on - strand at 1257.756 kb on - strand at 1257.757 kb on + strand at 1257.810 kb on + strand at 1257.812 kb on + strand at 1257.812 kb on + strand at 1257.853 kb on + strand, within MMP_RS06545 at 1257.877 kb on - strand, within MMP_RS06545 at 1257.922 kb on + strand, within MMP_RS06545 at 1257.929 kb on - strand, within MMP_RS06545 at 1257.963 kb on - strand, within MMP_RS06545 at 1258.027 kb on + strand, within MMP_RS06545 at 1258.109 kb on + strand at 1258.130 kb on - strand at 1258.139 kb on - strand at 1258.143 kb on - strand at 1258.320 kb on + strand, within MMP_RS06550 at 1258.406 kb on + strand, within MMP_RS06550 at 1258.670 kb on - strand, within MMP_RS06550 at 1258.676 kb on - strand, within MMP_RS06550 at 1258.733 kb on + strand, within MMP_RS06550 at 1258.733 kb on + strand, within MMP_RS06550
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,255,766 - MMP_RS06535 0.32 -2.2 1,255,766 - MMP_RS06535 0.32 +0.4 1,255,912 - MMP_RS06535 0.42 -0.7 1,256,538 + MMP_RS06535 0.85 -0.4 1,256,538 + MMP_RS06535 0.85 -0.6 1,256,584 + MMP_RS06535 0.88 -0.2 1,257,081 + MMP_RS06540 0.31 +0.3 1,257,087 - MMP_RS06540 0.32 +0.5 1,257,685 + MMP_RS06540 0.88 +1.7 1,257,705 + -0.2 1,257,738 - -0.5 1,257,756 - +0.8 1,257,756 - +1.2 1,257,757 + +0.9 1,257,810 + +0.8 1,257,812 + +0.8 1,257,812 + -0.5 1,257,853 + MMP_RS06545 0.14 +2.2 1,257,877 - MMP_RS06545 0.24 +0.1 1,257,922 + MMP_RS06545 0.43 +0.5 1,257,929 - MMP_RS06545 0.46 +0.9 1,257,963 - MMP_RS06545 0.60 +0.0 1,258,027 + MMP_RS06545 0.87 +1.2 1,258,109 + +0.5 1,258,130 - +1.1 1,258,139 - +0.2 1,258,143 - -0.3 1,258,320 + MMP_RS06550 0.11 +0.5 1,258,406 + MMP_RS06550 0.16 -1.6 1,258,670 - MMP_RS06550 0.32 +0.3 1,258,676 - MMP_RS06550 0.32 +1.0 1,258,733 + MMP_RS06550 0.35 -0.4 1,258,733 + MMP_RS06550 0.35 -0.8
Or see this region's nucleotide sequence