Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06300 and MMP_RS06305 are separated by 115 nucleotides MMP_RS06305 and MMP_RS06310 are separated by 19 nucleotides
MMP_RS06300: MMP_RS06300 - ABC transporter substrate-binding protein, at 1,213,682 to 1,214,446
_RS06300
MMP_RS06305: MMP_RS06305 - transporter substrate-binding domain-containing protein, at 1,214,562 to 1,215,326
_RS06305
MMP_RS06310: MMP_RS06310 - NAD(P)/FAD-dependent oxidoreductase, at 1,215,346 to 1,216,503
_RS06310
Position (kb) 1214
1215
1216 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 1213.614 kb on + strand at 1213.618 kb on + strand at 1213.628 kb on + strand at 1213.629 kb on - strand at 1213.630 kb on + strand at 1213.640 kb on - strand at 1213.647 kb on - strand at 1213.648 kb on + strand at 1213.651 kb on + strand at 1213.651 kb on + strand at 1213.654 kb on + strand at 1213.708 kb on + strand at 1213.747 kb on - strand at 1213.748 kb on + strand at 1213.779 kb on - strand, within MMP_RS06300 at 1213.833 kb on + strand, within MMP_RS06300 at 1214.030 kb on - strand, within MMP_RS06300 at 1214.030 kb on - strand, within MMP_RS06300 at 1214.130 kb on + strand, within MMP_RS06300 at 1214.130 kb on + strand, within MMP_RS06300 at 1214.187 kb on + strand, within MMP_RS06300 at 1214.217 kb on - strand, within MMP_RS06300 at 1214.218 kb on + strand, within MMP_RS06300 at 1214.220 kb on + strand, within MMP_RS06300 at 1214.230 kb on - strand, within MMP_RS06300 at 1214.242 kb on + strand, within MMP_RS06300 at 1214.539 kb on + strand at 1214.539 kb on + strand at 1214.546 kb on + strand at 1214.678 kb on + strand, within MMP_RS06305 at 1214.703 kb on - strand, within MMP_RS06305 at 1214.704 kb on + strand, within MMP_RS06305 at 1214.847 kb on + strand, within MMP_RS06305 at 1215.060 kb on - strand, within MMP_RS06305 at 1215.159 kb on + strand, within MMP_RS06305 at 1215.175 kb on + strand, within MMP_RS06305 at 1215.218 kb on - strand, within MMP_RS06305 at 1215.258 kb on + strand at 1215.259 kb on - strand at 1215.484 kb on - strand, within MMP_RS06310 at 1215.648 kb on + strand, within MMP_RS06310 at 1215.664 kb on - strand, within MMP_RS06310 at 1215.895 kb on + strand, within MMP_RS06310 at 1215.928 kb on - strand, within MMP_RS06310 at 1216.012 kb on + strand, within MMP_RS06310 at 1216.100 kb on + strand, within MMP_RS06310 at 1216.128 kb on - strand, within MMP_RS06310 at 1216.129 kb on + strand, within MMP_RS06310 at 1216.192 kb on + strand, within MMP_RS06310
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,213,614 + -0.1 1,213,618 + +0.7 1,213,628 + +0.9 1,213,629 - +0.6 1,213,630 + -0.7 1,213,640 - +0.3 1,213,647 - -0.2 1,213,648 + +0.2 1,213,651 + +0.9 1,213,651 + +0.7 1,213,654 + +0.5 1,213,708 + -0.0 1,213,747 - +0.5 1,213,748 + +0.4 1,213,779 - MMP_RS06300 0.13 +0.2 1,213,833 + MMP_RS06300 0.20 -0.3 1,214,030 - MMP_RS06300 0.45 +1.8 1,214,030 - MMP_RS06300 0.45 +1.0 1,214,130 + MMP_RS06300 0.59 -0.0 1,214,130 + MMP_RS06300 0.59 +0.5 1,214,187 + MMP_RS06300 0.66 -0.2 1,214,217 - MMP_RS06300 0.70 +0.6 1,214,218 + MMP_RS06300 0.70 -0.2 1,214,220 + MMP_RS06300 0.70 -0.6 1,214,230 - MMP_RS06300 0.72 +1.2 1,214,242 + MMP_RS06300 0.73 +1.6 1,214,539 + +0.2 1,214,539 + -0.6 1,214,546 + +0.0 1,214,678 + MMP_RS06305 0.15 +0.8 1,214,703 - MMP_RS06305 0.18 +0.7 1,214,704 + MMP_RS06305 0.19 -0.4 1,214,847 + MMP_RS06305 0.37 -0.4 1,215,060 - MMP_RS06305 0.65 +0.6 1,215,159 + MMP_RS06305 0.78 +1.2 1,215,175 + MMP_RS06305 0.80 +0.9 1,215,218 - MMP_RS06305 0.86 -0.0 1,215,258 + +0.2 1,215,259 - +1.3 1,215,484 - MMP_RS06310 0.12 -1.4 1,215,648 + MMP_RS06310 0.26 -1.6 1,215,664 - MMP_RS06310 0.27 +0.6 1,215,895 + MMP_RS06310 0.47 -2.7 1,215,928 - MMP_RS06310 0.50 -2.6 1,216,012 + MMP_RS06310 0.58 +1.0 1,216,100 + MMP_RS06310 0.65 -3.2 1,216,128 - MMP_RS06310 0.68 -0.7 1,216,129 + MMP_RS06310 0.68 -4.8 1,216,192 + MMP_RS06310 0.73 +0.4
Or see this region's nucleotide sequence