Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06240 and MMP_RS06245 are separated by 38 nucleotides MMP_RS06245 and MMP_RS06250 are separated by 41 nucleotides MMP_RS06250 and MMP_RS06255 are separated by 98 nucleotides
MMP_RS06240: MMP_RS06240 - thiolase domain-containing protein, at 1,199,123 to 1,200,301
_RS06240
MMP_RS06245: MMP_RS06245 - Zn-ribbon domain-containing OB-fold protein, at 1,200,340 to 1,200,732
_RS06245
MMP_RS06250: MMP_RS06250 - hypothetical protein, at 1,200,774 to 1,201,451
_RS06250
MMP_RS06255: MMP_RS06255 - cobyric acid synthase CobQ, at 1,201,550 to 1,203,028
_RS06255
Position (kb)
1200
1201
1202 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1199.888 kb on + strand at 1200.236 kb on + strand at 1200.312 kb on + strand at 1200.766 kb on + strand at 1200.776 kb on + strand at 1200.780 kb on + strand at 1200.826 kb on - strand at 1200.857 kb on + strand, within MMP_RS06250 at 1200.917 kb on - strand, within MMP_RS06250 at 1201.002 kb on - strand, within MMP_RS06250 at 1201.150 kb on + strand, within MMP_RS06250 at 1201.279 kb on + strand, within MMP_RS06250 at 1201.329 kb on + strand, within MMP_RS06250 at 1201.333 kb on - strand, within MMP_RS06250 at 1201.333 kb on - strand, within MMP_RS06250 at 1201.334 kb on + strand, within MMP_RS06250 at 1201.340 kb on - strand, within MMP_RS06250 at 1201.415 kb on + strand at 1201.418 kb on + strand at 1201.477 kb on - strand at 1201.478 kb on + strand at 1201.514 kb on - strand at 1201.546 kb on - strand at 1201.546 kb on - strand at 1201.559 kb on + strand at 1201.589 kb on - strand at 1201.632 kb on - strand at 1201.683 kb on + strand at 1201.895 kb on - strand at 1202.383 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,199,888 + -1.5 1,200,236 + +1.6 1,200,312 + -0.4 1,200,766 + -0.2 1,200,776 + -1.0 1,200,780 + +0.1 1,200,826 - +0.5 1,200,857 + MMP_RS06250 0.12 +0.0 1,200,917 - MMP_RS06250 0.21 -0.0 1,201,002 - MMP_RS06250 0.34 +0.1 1,201,150 + MMP_RS06250 0.55 -0.7 1,201,279 + MMP_RS06250 0.74 +0.3 1,201,329 + MMP_RS06250 0.82 +0.8 1,201,333 - MMP_RS06250 0.82 +0.8 1,201,333 - MMP_RS06250 0.82 +0.8 1,201,334 + MMP_RS06250 0.83 +0.6 1,201,340 - MMP_RS06250 0.83 +1.9 1,201,415 + -1.2 1,201,418 + +0.2 1,201,477 - +0.0 1,201,478 + +1.0 1,201,514 - -0.2 1,201,546 - -1.4 1,201,546 - +1.3 1,201,559 + +1.1 1,201,589 - +1.1 1,201,632 - -1.5 1,201,683 + -2.5 1,201,895 - -1.8 1,202,383 - -4.6
Or see this region's nucleotide sequence