Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05355 and MMP_RS05360 are separated by 37 nucleotides MMP_RS05360 and MMP_RS05365 are separated by 81 nucleotides MMP_RS05365 and MMP_RS05370 are separated by 417 nucleotides MMP_RS05370 and MMP_RS05375 are separated by 33 nucleotides
MMP_RS05355: MMP_RS05355 - hypothetical protein, at 1,029,175 to 1,029,588
_RS05355
MMP_RS05360: MMP_RS05360 - hypothetical protein, at 1,029,626 to 1,030,432
_RS05360
MMP_RS05365: MMP_RS05365 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH, at 1,030,514 to 1,030,993
_RS05365
MMP_RS05370: MMP_RS05370 - hypothetical protein, at 1,031,411 to 1,031,719
_RS05370
MMP_RS05375: MMP_RS05375 - V-type ATP synthase subunit I, at 1,031,753 to 1,033,813
_RS05375
Position (kb)
1030
1031 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1029.543 kb on + strand, within MMP_RS05355 at 1029.564 kb on + strand at 1029.564 kb on + strand at 1029.578 kb on - strand at 1029.625 kb on - strand at 1029.626 kb on + strand at 1029.626 kb on + strand at 1029.626 kb on + strand at 1029.649 kb on + strand at 1029.652 kb on + strand at 1029.655 kb on - strand at 1029.719 kb on + strand, within MMP_RS05360 at 1029.720 kb on - strand, within MMP_RS05360 at 1029.740 kb on + strand, within MMP_RS05360 at 1029.763 kb on - strand, within MMP_RS05360 at 1029.807 kb on - strand, within MMP_RS05360 at 1030.027 kb on - strand, within MMP_RS05360 at 1030.362 kb on - strand at 1030.363 kb on + strand at 1030.363 kb on + strand at 1030.396 kb on - strand at 1030.399 kb on + strand at 1030.514 kb on + strand at 1030.521 kb on - strand at 1030.522 kb on + strand at 1030.822 kb on + strand, within MMP_RS05365 at 1030.939 kb on + strand, within MMP_RS05365 at 1030.988 kb on + strand at 1031.128 kb on + strand at 1031.129 kb on - strand at 1031.130 kb on + strand at 1031.132 kb on - strand at 1031.140 kb on - strand at 1031.141 kb on + strand at 1031.227 kb on - strand at 1031.227 kb on - strand at 1031.269 kb on - strand at 1031.269 kb on - strand at 1031.270 kb on + strand at 1031.270 kb on + strand at 1031.270 kb on + strand at 1031.273 kb on - strand at 1031.323 kb on + strand at 1031.323 kb on + strand at 1031.349 kb on - strand at 1031.358 kb on + strand at 1031.368 kb on + strand at 1031.422 kb on + strand at 1031.422 kb on + strand at 1031.896 kb on - strand at 1031.922 kb on + strand at 1031.960 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 1,029,543 + MMP_RS05355 0.89 +0.4 1,029,564 + +0.5 1,029,564 + -1.2 1,029,578 - +0.3 1,029,625 - +1.2 1,029,626 + +0.4 1,029,626 + +0.5 1,029,626 + -0.9 1,029,649 + -0.7 1,029,652 + -0.5 1,029,655 - -1.1 1,029,719 + MMP_RS05360 0.12 -0.2 1,029,720 - MMP_RS05360 0.12 +0.3 1,029,740 + MMP_RS05360 0.14 -1.7 1,029,763 - MMP_RS05360 0.17 +0.7 1,029,807 - MMP_RS05360 0.22 -0.5 1,030,027 - MMP_RS05360 0.50 +0.2 1,030,362 - -0.1 1,030,363 + +1.0 1,030,363 + -1.0 1,030,396 - +2.0 1,030,399 + -0.5 1,030,514 + -0.1 1,030,521 - +0.8 1,030,522 + -1.2 1,030,822 + MMP_RS05365 0.64 +0.5 1,030,939 + MMP_RS05365 0.89 +0.8 1,030,988 + -0.3 1,031,128 + +0.3 1,031,129 - -0.9 1,031,130 + +0.0 1,031,132 - -0.6 1,031,140 - -0.5 1,031,141 + +0.2 1,031,227 - +0.2 1,031,227 - -0.0 1,031,269 - +0.3 1,031,269 - +0.1 1,031,270 + -1.7 1,031,270 + +0.1 1,031,270 + -0.2 1,031,273 - -0.4 1,031,323 + -2.7 1,031,323 + -1.1 1,031,349 - -2.5 1,031,358 + -3.9 1,031,368 + -4.6 1,031,422 + -2.3 1,031,422 + -3.6 1,031,896 - -2.6 1,031,922 + -4.3 1,031,960 + -2.8
Or see this region's nucleotide sequence