Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02400 and MMP_RS02405 are separated by 127 nucleotides MMP_RS02405 and MMP_RS02410 are separated by 115 nucleotides MMP_RS02410 and MMP_RS02415 are separated by 103 nucleotides
MMP_RS02400: MMP_RS02400 - site-2 protease family protein, at 445,925 to 446,938
_RS02400
MMP_RS02405: MMP_RS02405 - hypothetical protein, at 447,066 to 447,587
_RS02405
MMP_RS02410: MMP_RS02410 - ABC transporter substrate-binding protein, at 447,703 to 448,449
_RS02410
MMP_RS02415: MMP_RS02415 - site-specific integrase, at 448,553 to 449,101
_RS02415
Position (kb)
447
448
449 Strain fitness (log2 ratio)
-1
0
1
2 at 446.775 kb on - strand, within MMP_RS02400 at 446.776 kb on + strand, within MMP_RS02400 at 446.824 kb on + strand, within MMP_RS02400 at 446.877 kb on + strand at 446.962 kb on - strand at 446.962 kb on - strand at 446.962 kb on - strand at 446.962 kb on - strand at 446.963 kb on + strand at 446.963 kb on + strand at 446.963 kb on + strand at 446.965 kb on - strand at 446.965 kb on - strand at 446.965 kb on - strand at 446.966 kb on + strand at 446.966 kb on + strand at 446.968 kb on + strand at 446.968 kb on + strand at 446.988 kb on + strand at 447.006 kb on - strand at 447.007 kb on + strand at 447.007 kb on + strand at 447.010 kb on - strand at 447.010 kb on - strand at 447.010 kb on - strand at 447.010 kb on - strand at 447.020 kb on + strand at 447.050 kb on - strand at 447.051 kb on + strand at 447.085 kb on - strand at 447.085 kb on - strand at 447.085 kb on - strand at 447.366 kb on - strand, within MMP_RS02405 at 447.366 kb on - strand, within MMP_RS02405 at 447.367 kb on + strand, within MMP_RS02405 at 447.367 kb on + strand, within MMP_RS02405 at 447.369 kb on + strand, within MMP_RS02405 at 447.415 kb on + strand, within MMP_RS02405 at 447.472 kb on - strand, within MMP_RS02405 at 447.601 kb on - strand at 447.601 kb on - strand at 447.642 kb on + strand at 447.738 kb on + strand at 447.837 kb on - strand, within MMP_RS02410 at 448.088 kb on + strand, within MMP_RS02410 at 448.362 kb on - strand, within MMP_RS02410 at 448.392 kb on - strand at 448.483 kb on - strand at 448.483 kb on - strand at 448.483 kb on - strand at 448.947 kb on + strand, within MMP_RS02415 at 448.968 kb on + strand, within MMP_RS02415
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 446,775 - MMP_RS02400 0.84 -0.1 446,776 + MMP_RS02400 0.84 -0.7 446,824 + MMP_RS02400 0.89 -0.1 446,877 + +0.7 446,962 - +0.1 446,962 - +0.5 446,962 - +0.4 446,962 - +1.2 446,963 + +0.8 446,963 + -0.2 446,963 + +0.4 446,965 - +0.2 446,965 - +0.5 446,965 - -0.6 446,966 + -0.4 446,966 + -0.1 446,968 + +0.9 446,968 + +0.5 446,988 + -0.2 447,006 - -0.8 447,007 + +1.6 447,007 + +0.7 447,010 - +0.6 447,010 - -0.0 447,010 - -0.9 447,010 - +0.4 447,020 + +0.7 447,050 - -0.8 447,051 + -0.2 447,085 - +0.6 447,085 - +0.6 447,085 - +1.8 447,366 - MMP_RS02405 0.57 +1.0 447,366 - MMP_RS02405 0.57 +0.3 447,367 + MMP_RS02405 0.58 -1.1 447,367 + MMP_RS02405 0.58 +0.8 447,369 + MMP_RS02405 0.58 +0.3 447,415 + MMP_RS02405 0.67 +0.7 447,472 - MMP_RS02405 0.78 -0.5 447,601 - +1.2 447,601 - +1.0 447,642 + -0.2 447,738 + -0.6 447,837 - MMP_RS02410 0.18 -0.1 448,088 + MMP_RS02410 0.52 +1.1 448,362 - MMP_RS02410 0.88 +0.3 448,392 - +0.2 448,483 - +0.1 448,483 - +0.9 448,483 - -1.3 448,947 + MMP_RS02415 0.72 -0.4 448,968 + MMP_RS02415 0.76 +0.2
Or see this region's nucleotide sequence