Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02365 and MMP_RS02370 are separated by 213 nucleotides MMP_RS02370 and MMP_RS02375 are separated by 33 nucleotides MMP_RS02375 and MMP_RS02380 are separated by 156 nucleotides
MMP_RS02365: MMP_RS02365 - nitrogen fixation, at 441,990 to 442,316
_RS02365
MMP_RS02370: MMP_RS02370 - NifB/NifX family molybdenum-iron cluster-binding protein, at 442,530 to 442,889
_RS02370
MMP_RS02375: MMP_RS02375 - hypothetical protein, at 442,923 to 443,207
_RS02375
MMP_RS02380: MMP_RS02380 - ATP-binding protein, at 443,364 to 444,209
_RS02380
MMP_RS02385: MMP_RS02385 - ATP-binding protein, at 444,206 to 445,063
_RS02385
Position (kb) 442
443
444 Strain fitness (log2 ratio) -1
0
1
2
3 at 441.996 kb on + strand at 442.017 kb on + strand at 442.020 kb on - strand at 442.046 kb on - strand, within MMP_RS02365 at 442.068 kb on - strand, within MMP_RS02365 at 442.068 kb on - strand, within MMP_RS02365 at 442.069 kb on + strand, within MMP_RS02365 at 442.120 kb on - strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.121 kb on + strand, within MMP_RS02365 at 442.122 kb on - strand, within MMP_RS02365 at 442.123 kb on + strand, within MMP_RS02365 at 442.123 kb on + strand, within MMP_RS02365 at 442.128 kb on - strand, within MMP_RS02365 at 442.129 kb on + strand, within MMP_RS02365 at 442.219 kb on + strand, within MMP_RS02365 at 442.219 kb on + strand, within MMP_RS02365 at 442.248 kb on - strand, within MMP_RS02365 at 442.297 kb on + strand at 442.361 kb on - strand at 442.361 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.418 kb on - strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.419 kb on + strand at 442.422 kb on - strand at 442.423 kb on + strand at 442.455 kb on + strand at 442.481 kb on - strand at 442.481 kb on - strand at 442.483 kb on - strand at 442.484 kb on + strand at 442.488 kb on + strand at 442.490 kb on - strand at 442.494 kb on - strand at 442.557 kb on - strand at 442.581 kb on - strand, within MMP_RS02370 at 442.582 kb on + strand, within MMP_RS02370 at 442.605 kb on - strand, within MMP_RS02370 at 442.605 kb on - strand, within MMP_RS02370 at 442.662 kb on - strand, within MMP_RS02370 at 442.788 kb on - strand, within MMP_RS02370 at 442.870 kb on + strand at 442.882 kb on + strand at 443.035 kb on + strand, within MMP_RS02375 at 443.040 kb on + strand, within MMP_RS02375 at 443.043 kb on - strand, within MMP_RS02375 at 443.044 kb on + strand, within MMP_RS02375 at 443.058 kb on - strand, within MMP_RS02375 at 443.060 kb on - strand, within MMP_RS02375 at 443.061 kb on + strand, within MMP_RS02375 at 443.088 kb on + strand, within MMP_RS02375 at 443.145 kb on - strand, within MMP_RS02375 at 443.149 kb on - strand, within MMP_RS02375 at 443.159 kb on - strand, within MMP_RS02375 at 443.159 kb on - strand, within MMP_RS02375 at 443.163 kb on - strand, within MMP_RS02375 at 443.227 kb on + strand at 443.231 kb on - strand at 443.231 kb on - strand at 443.231 kb on - strand at 443.231 kb on - strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.232 kb on + strand at 443.240 kb on + strand at 443.242 kb on - strand at 443.274 kb on - strand at 443.293 kb on + strand at 443.294 kb on - strand at 443.574 kb on - strand, within MMP_RS02380 at 443.575 kb on + strand, within MMP_RS02380 at 443.769 kb on + strand, within MMP_RS02380 at 443.807 kb on - strand, within MMP_RS02380 at 443.854 kb on - strand, within MMP_RS02380 at 443.953 kb on + strand, within MMP_RS02380 at 444.023 kb on - strand, within MMP_RS02380 at 444.081 kb on - strand, within MMP_RS02380
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 441,996 + +0.9 442,017 + +0.3 442,020 - +0.7 442,046 - MMP_RS02365 0.17 -0.1 442,068 - MMP_RS02365 0.24 +1.0 442,068 - MMP_RS02365 0.24 -0.9 442,069 + MMP_RS02365 0.24 +0.5 442,120 - MMP_RS02365 0.40 +1.0 442,121 + MMP_RS02365 0.40 +2.0 442,121 + MMP_RS02365 0.40 -0.3 442,121 + MMP_RS02365 0.40 -1.5 442,122 - MMP_RS02365 0.40 -1.6 442,123 + MMP_RS02365 0.41 +1.1 442,123 + MMP_RS02365 0.41 +0.7 442,128 - MMP_RS02365 0.42 +2.0 442,129 + MMP_RS02365 0.43 -1.2 442,219 + MMP_RS02365 0.70 -0.4 442,219 + MMP_RS02365 0.70 +0.3 442,248 - MMP_RS02365 0.79 +0.9 442,297 + -0.4 442,361 - +0.3 442,361 - +0.2 442,418 - -0.5 442,418 - +0.2 442,418 - +0.7 442,418 - -0.9 442,418 - +0.8 442,418 - +0.5 442,418 - +1.1 442,418 - +0.2 442,418 - -0.2 442,418 - +1.0 442,418 - +0.3 442,418 - -0.2 442,418 - +1.4 442,418 - +0.6 442,419 + +0.5 442,419 + -1.0 442,419 + +0.2 442,419 + +0.4 442,419 + +0.4 442,419 + +0.9 442,422 - +0.4 442,423 + +1.4 442,455 + -0.4 442,481 - -0.7 442,481 - +0.2 442,483 - +0.3 442,484 + -1.3 442,488 + +0.1 442,490 - -0.5 442,494 - +0.6 442,557 - +0.2 442,581 - MMP_RS02370 0.14 +1.2 442,582 + MMP_RS02370 0.14 -0.2 442,605 - MMP_RS02370 0.21 +1.5 442,605 - MMP_RS02370 0.21 -0.2 442,662 - MMP_RS02370 0.37 +0.1 442,788 - MMP_RS02370 0.72 +0.9 442,870 + +0.5 442,882 + +0.1 443,035 + MMP_RS02375 0.39 +0.8 443,040 + MMP_RS02375 0.41 +1.4 443,043 - MMP_RS02375 0.42 +0.7 443,044 + MMP_RS02375 0.42 +0.4 443,058 - MMP_RS02375 0.47 +0.2 443,060 - MMP_RS02375 0.48 +1.2 443,061 + MMP_RS02375 0.48 -0.8 443,088 + MMP_RS02375 0.58 +1.0 443,145 - MMP_RS02375 0.78 +0.8 443,149 - MMP_RS02375 0.79 +0.2 443,159 - MMP_RS02375 0.83 +0.6 443,159 - MMP_RS02375 0.83 +0.6 443,163 - MMP_RS02375 0.84 +0.7 443,227 + -0.4 443,231 - +1.4 443,231 - +2.8 443,231 - +1.0 443,231 - +0.4 443,232 + +0.4 443,232 + -0.9 443,232 + +1.0 443,232 + +0.2 443,232 + +0.6 443,232 + +0.9 443,240 + -1.0 443,242 - -0.6 443,274 - +1.1 443,293 + -0.4 443,294 - +1.2 443,574 - MMP_RS02380 0.25 -0.0 443,575 + MMP_RS02380 0.25 +1.2 443,769 + MMP_RS02380 0.48 +0.7 443,807 - MMP_RS02380 0.52 -0.6 443,854 - MMP_RS02380 0.58 +0.4 443,953 + MMP_RS02380 0.70 +0.2 444,023 - MMP_RS02380 0.78 +0.5 444,081 - MMP_RS02380 0.85 -0.1
Or see this region's nucleotide sequence