Experiment: H2 N-free with 10mM L-Citrulline
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01135 and MMP_RS01140 are separated by 9 nucleotides MMP_RS01140 and MMP_RS01145 are separated by 9 nucleotides MMP_RS01145 and MMP_RS01150 are separated by 155 nucleotides
MMP_RS01135: MMP_RS01135 - molybdate ABC transporter substrate-binding protein, at 212,528 to 213,331
_RS01135
MMP_RS01140: MMP_RS01140 - molybdate ABC transporter permease subunit, at 213,341 to 214,006
_RS01140
MMP_RS01145: MMP_RS01145 - ABC transporter ATP-binding protein, at 214,016 to 215,158
_RS01145
MMP_RS01150: MMP_RS01150 - aldo/keto reductase, at 215,314 to 216,543
_RS01150
Position (kb) 214
215
216 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 213.129 kb on - strand, within MMP_RS01135 at 213.132 kb on + strand, within MMP_RS01135 at 213.132 kb on + strand, within MMP_RS01135 at 213.132 kb on + strand, within MMP_RS01135 at 213.175 kb on - strand, within MMP_RS01135 at 213.176 kb on + strand, within MMP_RS01135 at 213.176 kb on + strand, within MMP_RS01135 at 213.177 kb on - strand, within MMP_RS01135 at 213.177 kb on - strand, within MMP_RS01135 at 213.178 kb on + strand, within MMP_RS01135 at 213.206 kb on + strand, within MMP_RS01135 at 213.206 kb on + strand, within MMP_RS01135 at 213.217 kb on + strand, within MMP_RS01135 at 213.217 kb on + strand, within MMP_RS01135 at 213.220 kb on - strand, within MMP_RS01135 at 213.220 kb on - strand, within MMP_RS01135 at 213.269 kb on - strand at 213.270 kb on + strand at 213.336 kb on + strand at 213.354 kb on - strand at 213.355 kb on + strand at 213.355 kb on + strand at 213.366 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.370 kb on - strand at 213.388 kb on - strand at 213.390 kb on - strand at 213.395 kb on + strand at 213.395 kb on + strand at 213.395 kb on + strand at 213.480 kb on - strand, within MMP_RS01140 at 213.480 kb on - strand, within MMP_RS01140 at 213.597 kb on - strand, within MMP_RS01140 at 213.934 kb on + strand, within MMP_RS01140 at 213.949 kb on + strand at 214.004 kb on - strand at 214.016 kb on + strand at 214.114 kb on + strand at 214.262 kb on + strand, within MMP_RS01145 at 214.305 kb on + strand, within MMP_RS01145 at 214.408 kb on + strand, within MMP_RS01145 at 214.474 kb on + strand, within MMP_RS01145 at 214.782 kb on - strand, within MMP_RS01145 at 214.782 kb on - strand, within MMP_RS01145 at 214.903 kb on + strand, within MMP_RS01145 at 214.911 kb on - strand, within MMP_RS01145 at 214.911 kb on - strand, within MMP_RS01145 at 214.916 kb on + strand, within MMP_RS01145 at 214.917 kb on - strand, within MMP_RS01145 at 214.965 kb on - strand, within MMP_RS01145 at 214.965 kb on - strand, within MMP_RS01145 at 214.966 kb on + strand, within MMP_RS01145 at 215.062 kb on + strand at 215.217 kb on - strand at 215.218 kb on + strand at 215.218 kb on + strand at 215.218 kb on + strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.219 kb on - strand at 215.220 kb on + strand at 215.220 kb on + strand at 215.220 kb on + strand at 215.222 kb on + strand at 215.237 kb on - strand at 215.309 kb on - strand at 215.313 kb on - strand at 215.313 kb on - strand at 215.314 kb on + strand at 215.467 kb on + strand, within MMP_RS01150 at 215.603 kb on + strand, within MMP_RS01150 at 215.650 kb on - strand, within MMP_RS01150 at 215.835 kb on - strand, within MMP_RS01150
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Citrulline remove 213,129 - MMP_RS01135 0.75 +1.7 213,132 + MMP_RS01135 0.75 -0.4 213,132 + MMP_RS01135 0.75 +0.8 213,132 + MMP_RS01135 0.75 +2.3 213,175 - MMP_RS01135 0.80 +0.5 213,176 + MMP_RS01135 0.81 +0.4 213,176 + MMP_RS01135 0.81 +0.5 213,177 - MMP_RS01135 0.81 +0.3 213,177 - MMP_RS01135 0.81 +0.6 213,178 + MMP_RS01135 0.81 +1.2 213,206 + MMP_RS01135 0.84 +1.2 213,206 + MMP_RS01135 0.84 +0.2 213,217 + MMP_RS01135 0.86 +1.4 213,217 + MMP_RS01135 0.86 +1.1 213,220 - MMP_RS01135 0.86 +0.5 213,220 - MMP_RS01135 0.86 +0.9 213,269 - +0.7 213,270 + -0.4 213,336 + +0.7 213,354 - -0.5 213,355 + +1.1 213,355 + +0.2 213,366 - +1.0 213,370 - +0.4 213,370 - +0.6 213,370 - -0.6 213,370 - -1.5 213,370 - +0.0 213,388 - -0.3 213,390 - +1.2 213,395 + -0.4 213,395 + +2.8 213,395 + +0.0 213,480 - MMP_RS01140 0.21 -0.4 213,480 - MMP_RS01140 0.21 +0.3 213,597 - MMP_RS01140 0.38 -2.2 213,934 + MMP_RS01140 0.89 +0.8 213,949 + -0.5 214,004 - +1.4 214,016 + +0.8 214,114 + +0.7 214,262 + MMP_RS01145 0.22 -0.4 214,305 + MMP_RS01145 0.25 +1.2 214,408 + MMP_RS01145 0.34 -0.3 214,474 + MMP_RS01145 0.40 +1.5 214,782 - MMP_RS01145 0.67 +0.3 214,782 - MMP_RS01145 0.67 +1.4 214,903 + MMP_RS01145 0.78 -0.1 214,911 - MMP_RS01145 0.78 -0.3 214,911 - MMP_RS01145 0.78 -0.7 214,916 + MMP_RS01145 0.79 +1.5 214,917 - MMP_RS01145 0.79 +2.0 214,965 - MMP_RS01145 0.83 +1.2 214,965 - MMP_RS01145 0.83 +0.2 214,966 + MMP_RS01145 0.83 +1.6 215,062 + +1.5 215,217 - +1.4 215,218 + -0.0 215,218 + -0.1 215,218 + +0.3 215,219 - +0.6 215,219 - -0.4 215,219 - +0.9 215,219 - +0.2 215,219 - +0.6 215,219 - -1.2 215,219 - +1.3 215,220 + +0.1 215,220 + +0.0 215,220 + +0.2 215,222 + +1.0 215,237 - +0.4 215,309 - +0.9 215,313 - +0.9 215,313 - +1.1 215,314 + +1.1 215,467 + MMP_RS01150 0.12 +0.0 215,603 + MMP_RS01150 0.23 -0.5 215,650 - MMP_RS01150 0.27 +0.5 215,835 - MMP_RS01150 0.42 +1.6
Or see this region's nucleotide sequence