Experiment: H2 N-free with 10mM L-Isoleucine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS00910 and MMP_RS00915 are separated by 18 nucleotides MMP_RS00915 and MMP_RS00920 are separated by 60 nucleotides MMP_RS00920 and MMP_RS00925 are separated by 47 nucleotides MMP_RS00925 and MMP_RS00930 are separated by 90 nucleotides
MMP_RS00910: MMP_RS00910 - 2-phosphosulfolactate phosphatase, at 170,597 to 171,343
_RS00910
MMP_RS00915: MMP_RS00915 - DUF116 domain-containing protein, at 171,362 to 172,006
_RS00915
MMP_RS00920: MMP_RS00920 - TRC40/GET3/ArsA family transport-energizing ATPase, at 172,067 to 173,104
_RS00920
MMP_RS00925: MMP_RS00925 - sirohydrochlorin nickelochelatase, at 173,152 to 173,586
_RS00925
MMP_RS00930: MMP_RS00930 - ABC transporter permease, at 173,677 to 174,438
_RS00930
Position (kb) 172
173
174 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 171.086 kb on - strand, within MMP_RS00910 at 171.165 kb on + strand, within MMP_RS00910 at 171.194 kb on - strand, within MMP_RS00910 at 171.196 kb on - strand, within MMP_RS00910 at 171.196 kb on - strand, within MMP_RS00910 at 171.196 kb on - strand, within MMP_RS00910 at 171.197 kb on + strand, within MMP_RS00910 at 171.197 kb on + strand, within MMP_RS00910 at 171.198 kb on - strand, within MMP_RS00910 at 171.198 kb on - strand, within MMP_RS00910 at 171.198 kb on - strand, within MMP_RS00910 at 171.199 kb on + strand, within MMP_RS00910 at 171.200 kb on - strand, within MMP_RS00910 at 171.201 kb on + strand, within MMP_RS00910 at 171.221 kb on - strand, within MMP_RS00910 at 171.227 kb on + strand, within MMP_RS00910 at 171.260 kb on - strand, within MMP_RS00910 at 171.280 kb on - strand at 171.417 kb on - strand at 171.417 kb on - strand at 171.444 kb on - strand, within MMP_RS00915 at 171.445 kb on + strand, within MMP_RS00915 at 171.472 kb on - strand, within MMP_RS00915 at 171.473 kb on + strand, within MMP_RS00915 at 171.504 kb on - strand, within MMP_RS00915 at 171.512 kb on - strand, within MMP_RS00915 at 171.513 kb on + strand, within MMP_RS00915 at 171.517 kb on - strand, within MMP_RS00915 at 171.541 kb on + strand, within MMP_RS00915 at 171.559 kb on + strand, within MMP_RS00915 at 171.559 kb on + strand, within MMP_RS00915 at 171.611 kb on - strand, within MMP_RS00915 at 171.823 kb on + strand, within MMP_RS00915 at 171.909 kb on - strand, within MMP_RS00915 at 171.946 kb on - strand at 172.036 kb on + strand at 172.522 kb on + strand at 173.068 kb on - strand at 173.098 kb on + strand at 173.179 kb on - strand at 173.179 kb on - strand at 173.224 kb on - strand at 173.225 kb on + strand at 173.449 kb on + strand at 173.652 kb on + strand at 173.652 kb on + strand at 173.656 kb on + strand at 173.705 kb on - strand at 173.713 kb on - strand at 173.788 kb on - strand, within MMP_RS00930 at 173.816 kb on - strand, within MMP_RS00930 at 173.816 kb on - strand, within MMP_RS00930 at 173.817 kb on + strand, within MMP_RS00930 at 173.817 kb on + strand, within MMP_RS00930 at 173.832 kb on - strand, within MMP_RS00930 at 173.834 kb on - strand, within MMP_RS00930 at 173.911 kb on - strand, within MMP_RS00930
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Isoleucine remove 171,086 - MMP_RS00910 0.65 +0.9 171,165 + MMP_RS00910 0.76 -0.2 171,194 - MMP_RS00910 0.80 -0.8 171,196 - MMP_RS00910 0.80 -0.3 171,196 - MMP_RS00910 0.80 -0.3 171,196 - MMP_RS00910 0.80 +0.5 171,197 + MMP_RS00910 0.80 +0.9 171,197 + MMP_RS00910 0.80 -0.4 171,198 - MMP_RS00910 0.80 +1.6 171,198 - MMP_RS00910 0.80 +1.5 171,198 - MMP_RS00910 0.80 -2.4 171,199 + MMP_RS00910 0.81 -0.2 171,200 - MMP_RS00910 0.81 +1.2 171,201 + MMP_RS00910 0.81 -0.4 171,221 - MMP_RS00910 0.84 -0.0 171,227 + MMP_RS00910 0.84 +0.4 171,260 - MMP_RS00910 0.89 -0.6 171,280 - -0.9 171,417 - -4.6 171,417 - -2.8 171,444 - MMP_RS00915 0.13 -3.7 171,445 + MMP_RS00915 0.13 -3.2 171,472 - MMP_RS00915 0.17 -4.6 171,473 + MMP_RS00915 0.17 -0.5 171,504 - MMP_RS00915 0.22 -2.9 171,512 - MMP_RS00915 0.23 -4.0 171,513 + MMP_RS00915 0.23 -1.1 171,517 - MMP_RS00915 0.24 -0.0 171,541 + MMP_RS00915 0.28 -2.9 171,559 + MMP_RS00915 0.31 -2.1 171,559 + MMP_RS00915 0.31 -3.7 171,611 - MMP_RS00915 0.39 -2.4 171,823 + MMP_RS00915 0.71 -3.7 171,909 - MMP_RS00915 0.85 -0.5 171,946 - -4.1 172,036 + +0.2 172,522 + +2.3 173,068 - +0.4 173,098 + -3.9 173,179 - -0.7 173,179 - -3.6 173,224 - -2.1 173,225 + -0.2 173,449 + -1.8 173,652 + -0.6 173,652 + -2.3 173,656 + +0.5 173,705 - -0.3 173,713 - +1.2 173,788 - MMP_RS00930 0.15 -0.0 173,816 - MMP_RS00930 0.18 +1.5 173,816 - MMP_RS00930 0.18 -0.4 173,817 + MMP_RS00930 0.18 +0.2 173,817 + MMP_RS00930 0.18 +1.4 173,832 - MMP_RS00930 0.20 -0.3 173,834 - MMP_RS00930 0.21 -1.0 173,911 - MMP_RS00930 0.31 -0.2
Or see this region's nucleotide sequence