Experiment: H2 N-free with 10mM L-Valine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03420 and MMP_RS03425 are separated by 506 nucleotides MMP_RS03425 and MMP_RS03430 are separated by 21 nucleotides MMP_RS03430 and MMP_RS03435 are separated by 119 nucleotides
MMP_RS03420: MMP_RS03420 - acetolactate synthase small subunit, at 643,154 to 643,663
_RS03420
MMP_RS03425: MMP_RS03425 - hypothetical protein, at 644,170 to 644,622
_RS03425
MMP_RS03430: MMP_RS03430 - bile acid:sodium symporter family protein, at 644,644 to 645,609
_RS03430
MMP_RS03435: MMP_RS03435 - ketol-acid reductoisomerase, at 645,729 to 646,721
_RS03435
Position (kb) 644
645
646 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 643.655 kb on + strand at 643.765 kb on + strand at 643.903 kb on + strand at 643.972 kb on - strand at 643.987 kb on - strand at 643.988 kb on + strand at 644.071 kb on + strand at 644.090 kb on + strand at 644.090 kb on + strand at 644.129 kb on - strand at 644.145 kb on + strand at 644.147 kb on + strand at 644.262 kb on - strand, within MMP_RS03425 at 644.268 kb on - strand, within MMP_RS03425 at 644.307 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.324 kb on - strand, within MMP_RS03425 at 644.354 kb on + strand, within MMP_RS03425 at 644.419 kb on - strand, within MMP_RS03425 at 644.431 kb on + strand, within MMP_RS03425 at 644.485 kb on + strand, within MMP_RS03425 at 644.495 kb on + strand, within MMP_RS03425 at 644.562 kb on - strand, within MMP_RS03425 at 644.569 kb on - strand, within MMP_RS03425 at 644.621 kb on + strand at 644.864 kb on - strand, within MMP_RS03430 at 644.865 kb on + strand, within MMP_RS03430 at 644.895 kb on - strand, within MMP_RS03430 at 644.895 kb on - strand, within MMP_RS03430 at 644.971 kb on - strand, within MMP_RS03430 at 645.014 kb on + strand, within MMP_RS03430 at 645.042 kb on - strand, within MMP_RS03430 at 645.048 kb on - strand, within MMP_RS03430 at 645.107 kb on - strand, within MMP_RS03430 at 645.146 kb on + strand, within MMP_RS03430 at 645.230 kb on - strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.231 kb on + strand, within MMP_RS03430 at 645.314 kb on + strand, within MMP_RS03430 at 645.319 kb on + strand, within MMP_RS03430 at 645.417 kb on - strand, within MMP_RS03430 at 645.491 kb on + strand, within MMP_RS03430 at 645.683 kb on + strand at 645.700 kb on + strand at 645.737 kb on + strand at 645.755 kb on - strand at 645.917 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435 at 645.965 kb on + strand, within MMP_RS03435
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Valine remove 643,655 + +2.2 643,765 + -1.4 643,903 + +0.5 643,972 - -0.8 643,987 - +0.4 643,988 + +0.1 644,071 + -0.3 644,090 + +0.4 644,090 + +0.6 644,129 - -1.4 644,145 + -0.2 644,147 + +0.6 644,262 - MMP_RS03425 0.20 +0.4 644,268 - MMP_RS03425 0.22 +0.2 644,307 - MMP_RS03425 0.30 +1.2 644,324 - MMP_RS03425 0.34 -1.1 644,324 - MMP_RS03425 0.34 +1.3 644,354 + MMP_RS03425 0.41 +1.0 644,419 - MMP_RS03425 0.55 -0.3 644,431 + MMP_RS03425 0.58 +0.2 644,485 + MMP_RS03425 0.70 +0.6 644,495 + MMP_RS03425 0.72 -0.2 644,562 - MMP_RS03425 0.87 -0.1 644,569 - MMP_RS03425 0.88 +0.1 644,621 + -0.5 644,864 - MMP_RS03430 0.23 +1.1 644,865 + MMP_RS03430 0.23 +1.7 644,895 - MMP_RS03430 0.26 +1.3 644,895 - MMP_RS03430 0.26 +2.1 644,971 - MMP_RS03430 0.34 +2.1 645,014 + MMP_RS03430 0.38 -0.3 645,042 - MMP_RS03430 0.41 +1.3 645,048 - MMP_RS03430 0.42 +0.3 645,107 - MMP_RS03430 0.48 -0.1 645,146 + MMP_RS03430 0.52 +0.6 645,230 - MMP_RS03430 0.61 +1.2 645,231 + MMP_RS03430 0.61 -0.0 645,231 + MMP_RS03430 0.61 -0.9 645,231 + MMP_RS03430 0.61 +1.7 645,314 + MMP_RS03430 0.69 +0.4 645,319 + MMP_RS03430 0.70 +0.1 645,417 - MMP_RS03430 0.80 -0.2 645,491 + MMP_RS03430 0.88 +0.4 645,683 + +0.0 645,700 + -4.6 645,737 + -4.6 645,755 - -4.8 645,917 + MMP_RS03435 0.19 -3.7 645,965 + MMP_RS03435 0.24 -3.2 645,965 + MMP_RS03435 0.24 -3.2
Or see this region's nucleotide sequence