Experiment: H2 N-free with 10mM L-Valine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03185 and MMP_RS03190 are separated by 53 nucleotides MMP_RS03190 and MMP_RS03195 are separated by 24 nucleotides MMP_RS03195 and MMP_RS03200 are separated by 36 nucleotides
MMP_RS03185: MMP_RS03185 - KH domain-containing protein, at 600,046 to 600,600
_RS03185
MMP_RS03190: MMP_RS03190 - RlmE family RNA methyltransferase, at 600,654 to 601,430
_RS03190
MMP_RS03195: MMP_RS03195 - global nitrogen regulator NrpR, at 601,455 to 603,065
_RS03195
MMP_RS03200: MMP_RS03200 - methanogenesis marker 15 protein, at 603,102 to 604,337
_RS03200
Position (kb) 601
602
603
604 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 600.632 kb on - strand at 600.744 kb on + strand at 600.781 kb on + strand at 600.841 kb on - strand at 600.842 kb on + strand at 601.029 kb on - strand at 601.279 kb on + strand at 601.300 kb on + strand at 601.320 kb on - strand at 601.337 kb on + strand at 601.337 kb on + strand at 601.442 kb on - strand at 601.443 kb on + strand at 601.909 kb on - strand at 602.076 kb on + strand at 602.332 kb on + strand at 602.616 kb on + strand at 602.640 kb on - strand at 602.646 kb on + strand at 603.390 kb on - strand at 603.854 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Valine remove 600,632 - +0.2 600,744 + -2.3 600,781 + -2.5 600,841 - -0.9 600,842 + +0.3 601,029 - -2.1 601,279 + -0.9 601,300 + -3.9 601,320 - -0.8 601,337 + -2.2 601,337 + -2.1 601,442 - -2.3 601,443 + -3.3 601,909 - +1.2 602,076 + -0.2 602,332 + -0.6 602,616 + -0.1 602,640 - -0.3 602,646 + -0.4 603,390 - -0.2 603,854 - -2.1
Or see this region's nucleotide sequence