Experiment: H2 N-free with 10mM L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05640 and MMP_RS05645 are separated by 72 nucleotides MMP_RS05645 and MMP_RS05650 overlap by 4 nucleotides MMP_RS05650 and MMP_RS05655 are separated by 70 nucleotides
MMP_RS05640: MMP_RS05640 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,083,536 to 1,084,384
_RS05640
MMP_RS05645: MMP_RS05645 - AEC family transporter, at 1,084,457 to 1,085,371
_RS05645
MMP_RS05650: MMP_RS05650 - phosphopantetheine adenylyltransferase, at 1,085,368 to 1,085,814
_RS05650
MMP_RS05655: MMP_RS05655 - phosphoenolpyruvate synthase, at 1,085,885 to 1,088,161
_RS05655
Position (kb)
1084
1085
1086 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.458 kb on - strand at 1083.459 kb on + strand at 1083.459 kb on + strand at 1083.469 kb on - strand at 1083.469 kb on - strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.472 kb on + strand at 1083.489 kb on + strand at 1083.489 kb on + strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.490 kb on - strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.491 kb on + strand at 1083.497 kb on - strand at 1083.497 kb on - strand at 1083.532 kb on - strand at 1083.561 kb on - strand at 1083.623 kb on + strand, within MMP_RS05640 at 1083.708 kb on + strand, within MMP_RS05640 at 1083.773 kb on - strand, within MMP_RS05640 at 1083.774 kb on + strand, within MMP_RS05640 at 1083.778 kb on + strand, within MMP_RS05640 at 1083.816 kb on + strand, within MMP_RS05640 at 1083.816 kb on + strand, within MMP_RS05640 at 1083.892 kb on - strand, within MMP_RS05640 at 1084.008 kb on + strand, within MMP_RS05640 at 1084.087 kb on - strand, within MMP_RS05640 at 1084.088 kb on + strand, within MMP_RS05640 at 1084.089 kb on - strand, within MMP_RS05640 at 1084.092 kb on - strand, within MMP_RS05640 at 1084.222 kb on - strand, within MMP_RS05640 at 1084.235 kb on + strand, within MMP_RS05640 at 1084.268 kb on - strand, within MMP_RS05640 at 1084.269 kb on + strand, within MMP_RS05640 at 1084.272 kb on - strand, within MMP_RS05640 at 1084.273 kb on + strand, within MMP_RS05640 at 1084.278 kb on + strand, within MMP_RS05640 at 1084.389 kb on - strand at 1084.405 kb on - strand at 1084.405 kb on - strand at 1084.411 kb on + strand at 1084.414 kb on + strand at 1084.414 kb on + strand at 1084.416 kb on - strand at 1084.419 kb on - strand at 1084.428 kb on - strand at 1084.429 kb on + strand at 1084.459 kb on + strand at 1084.464 kb on + strand at 1084.530 kb on + strand at 1084.536 kb on + strand at 1084.542 kb on + strand at 1084.542 kb on + strand at 1084.548 kb on - strand at 1084.549 kb on + strand, within MMP_RS05645 at 1084.619 kb on - strand, within MMP_RS05645 at 1084.682 kb on + strand, within MMP_RS05645 at 1084.780 kb on - strand, within MMP_RS05645 at 1084.780 kb on - strand, within MMP_RS05645 at 1084.786 kb on - strand, within MMP_RS05645 at 1084.786 kb on - strand, within MMP_RS05645 at 1084.786 kb on - strand, within MMP_RS05645 at 1084.787 kb on + strand, within MMP_RS05645 at 1084.788 kb on - strand, within MMP_RS05645 at 1084.788 kb on - strand, within MMP_RS05645 at 1084.789 kb on + strand, within MMP_RS05645 at 1084.789 kb on + strand, within MMP_RS05645 at 1084.821 kb on + strand, within MMP_RS05645 at 1084.827 kb on + strand, within MMP_RS05645 at 1084.842 kb on + strand, within MMP_RS05645 at 1084.859 kb on + strand, within MMP_RS05645 at 1084.941 kb on - strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.942 kb on + strand, within MMP_RS05645 at 1084.978 kb on - strand, within MMP_RS05645 at 1085.158 kb on - strand, within MMP_RS05645 at 1085.166 kb on + strand, within MMP_RS05645 at 1085.212 kb on - strand, within MMP_RS05645 at 1085.215 kb on - strand, within MMP_RS05645 at 1085.349 kb on - strand at 1085.349 kb on - strand at 1085.349 kb on - strand at 1085.350 kb on + strand at 1085.350 kb on + strand at 1085.362 kb on - strand at 1085.552 kb on + strand at 1085.626 kb on + strand at 1085.712 kb on - strand at 1085.782 kb on + strand at 1085.790 kb on - strand at 1086.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Cysteine remove 1,083,458 - +0.6 1,083,458 - +0.4 1,083,458 - +0.1 1,083,459 + +0.4 1,083,459 + +0.1 1,083,469 - +0.5 1,083,469 - +0.1 1,083,472 + +0.8 1,083,472 + -0.0 1,083,472 + +0.2 1,083,489 + -0.7 1,083,489 + -0.3 1,083,490 - -0.7 1,083,490 - +0.0 1,083,490 - +0.0 1,083,491 + -0.0 1,083,491 + -0.2 1,083,491 + -0.4 1,083,491 + +0.8 1,083,497 - -0.0 1,083,497 - -0.2 1,083,532 - -0.3 1,083,561 - -0.1 1,083,623 + MMP_RS05640 0.10 -3.7 1,083,708 + MMP_RS05640 0.20 -1.4 1,083,773 - MMP_RS05640 0.28 -2.6 1,083,774 + MMP_RS05640 0.28 +1.6 1,083,778 + MMP_RS05640 0.29 +0.5 1,083,816 + MMP_RS05640 0.33 -0.2 1,083,816 + MMP_RS05640 0.33 -0.3 1,083,892 - MMP_RS05640 0.42 +0.3 1,084,008 + MMP_RS05640 0.56 +0.0 1,084,087 - MMP_RS05640 0.65 -0.4 1,084,088 + MMP_RS05640 0.65 -1.4 1,084,089 - MMP_RS05640 0.65 -2.8 1,084,092 - MMP_RS05640 0.65 -0.3 1,084,222 - MMP_RS05640 0.81 +0.1 1,084,235 + MMP_RS05640 0.82 -2.7 1,084,268 - MMP_RS05640 0.86 -0.1 1,084,269 + MMP_RS05640 0.86 +0.3 1,084,272 - MMP_RS05640 0.87 +0.5 1,084,273 + MMP_RS05640 0.87 -0.4 1,084,278 + MMP_RS05640 0.87 -0.0 1,084,389 - +0.1 1,084,405 - -0.7 1,084,405 - -0.0 1,084,411 + -1.2 1,084,414 + +1.0 1,084,414 + +0.2 1,084,416 - +0.2 1,084,419 - +0.2 1,084,428 - +0.5 1,084,429 + +0.4 1,084,459 + -0.7 1,084,464 + -1.9 1,084,530 + +0.8 1,084,536 + -0.0 1,084,542 + -0.2 1,084,542 + -0.4 1,084,548 - +0.7 1,084,549 + MMP_RS05645 0.10 +0.1 1,084,619 - MMP_RS05645 0.18 +0.6 1,084,682 + MMP_RS05645 0.25 -0.5 1,084,780 - MMP_RS05645 0.35 -0.4 1,084,780 - MMP_RS05645 0.35 -0.2 1,084,786 - MMP_RS05645 0.36 +0.1 1,084,786 - MMP_RS05645 0.36 -0.6 1,084,786 - MMP_RS05645 0.36 -0.6 1,084,787 + MMP_RS05645 0.36 +0.5 1,084,788 - MMP_RS05645 0.36 -0.5 1,084,788 - MMP_RS05645 0.36 +0.0 1,084,789 + MMP_RS05645 0.36 +0.5 1,084,789 + MMP_RS05645 0.36 -0.8 1,084,821 + MMP_RS05645 0.40 -0.3 1,084,827 + MMP_RS05645 0.40 -0.6 1,084,842 + MMP_RS05645 0.42 -0.2 1,084,859 + MMP_RS05645 0.44 -1.1 1,084,941 - MMP_RS05645 0.53 -0.2 1,084,942 + MMP_RS05645 0.53 +0.1 1,084,942 + MMP_RS05645 0.53 -0.5 1,084,942 + MMP_RS05645 0.53 -0.2 1,084,942 + MMP_RS05645 0.53 -2.5 1,084,978 - MMP_RS05645 0.57 -0.4 1,085,158 - MMP_RS05645 0.77 -0.2 1,085,166 + MMP_RS05645 0.77 +0.1 1,085,212 - MMP_RS05645 0.83 -0.4 1,085,215 - MMP_RS05645 0.83 -1.0 1,085,349 - +0.4 1,085,349 - +0.8 1,085,349 - -0.1 1,085,350 + -0.0 1,085,350 + -0.4 1,085,362 - -0.6 1,085,552 + -1.2 1,085,626 + -2.3 1,085,712 - -0.5 1,085,782 + +0.8 1,085,790 - +0.2 1,086,350 + -0.5
Or see this region's nucleotide sequence