Experiment: H2 N-free with 10mM L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05485 and MMP_RS05490 are separated by 15 nucleotides MMP_RS05490 and MMP_RS05495 are separated by 30 nucleotides MMP_RS05495 and MMP_RS05500 are separated by 83 nucleotides
MMP_RS05485: MMP_RS05485 - cysteine--tRNA ligase, at 1,051,556 to 1,052,986
_RS05485
MMP_RS05490: MMP_RS05490 - IMPACT family protein, at 1,053,002 to 1,053,610
_RS05490
MMP_RS05495: MMP_RS05495 - hypothetical protein, at 1,053,641 to 1,055,482
_RS05495
MMP_RS05500: MMP_RS05500 - 2-isopropylmalate synthase, at 1,055,566 to 1,057,110
_RS05500
Position (kb)
1053
1054
1055
1056 Strain fitness (log2 ratio)
-2
-1
0
1 at 1052.799 kb on + strand, within MMP_RS05485 at 1052.839 kb on - strand, within MMP_RS05485 at 1052.905 kb on + strand at 1053.210 kb on + strand, within MMP_RS05490 at 1053.327 kb on - strand, within MMP_RS05490 at 1053.376 kb on - strand, within MMP_RS05490 at 1053.416 kb on - strand, within MMP_RS05490 at 1053.458 kb on + strand, within MMP_RS05490 at 1053.463 kb on - strand, within MMP_RS05490 at 1053.500 kb on - strand, within MMP_RS05490 at 1053.599 kb on - strand at 1053.606 kb on - strand at 1053.656 kb on + strand at 1053.658 kb on + strand at 1053.673 kb on + strand at 1053.706 kb on + strand at 1053.713 kb on + strand at 1053.740 kb on + strand at 1053.944 kb on - strand, within MMP_RS05495 at 1054.235 kb on + strand, within MMP_RS05495 at 1054.248 kb on - strand, within MMP_RS05495 at 1054.272 kb on + strand, within MMP_RS05495 at 1054.324 kb on + strand, within MMP_RS05495 at 1054.367 kb on + strand, within MMP_RS05495 at 1054.425 kb on - strand, within MMP_RS05495 at 1054.517 kb on - strand, within MMP_RS05495 at 1054.520 kb on - strand, within MMP_RS05495 at 1054.620 kb on - strand, within MMP_RS05495 at 1054.636 kb on - strand, within MMP_RS05495 at 1054.669 kb on + strand, within MMP_RS05495 at 1054.737 kb on - strand, within MMP_RS05495 at 1054.936 kb on + strand, within MMP_RS05495 at 1054.936 kb on + strand, within MMP_RS05495 at 1055.011 kb on + strand, within MMP_RS05495 at 1055.011 kb on + strand, within MMP_RS05495 at 1055.012 kb on - strand, within MMP_RS05495 at 1055.060 kb on - strand, within MMP_RS05495 at 1055.060 kb on - strand, within MMP_RS05495 at 1055.060 kb on - strand, within MMP_RS05495 at 1055.061 kb on + strand, within MMP_RS05495 at 1055.061 kb on + strand, within MMP_RS05495 at 1055.061 kb on + strand, within MMP_RS05495 at 1055.072 kb on + strand, within MMP_RS05495 at 1055.083 kb on + strand, within MMP_RS05495 at 1055.269 kb on + strand, within MMP_RS05495 at 1055.409 kb on - strand at 1055.409 kb on - strand at 1055.414 kb on + strand at 1055.421 kb on + strand at 1055.424 kb on - strand at 1055.424 kb on - strand at 1055.458 kb on - strand at 1055.458 kb on - strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.459 kb on + strand at 1055.477 kb on + strand at 1055.523 kb on - strand at 1055.525 kb on - strand at 1055.561 kb on - strand at 1055.561 kb on - strand at 1055.576 kb on + strand at 1055.985 kb on - strand, within MMP_RS05500 at 1055.997 kb on + strand, within MMP_RS05500 at 1056.012 kb on + strand, within MMP_RS05500 at 1056.273 kb on + strand, within MMP_RS05500 at 1056.284 kb on - strand, within MMP_RS05500 at 1056.342 kb on + strand, within MMP_RS05500 at 1056.361 kb on + strand, within MMP_RS05500 at 1056.371 kb on - strand, within MMP_RS05500
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Cysteine remove 1,052,799 + MMP_RS05485 0.87 -0.0 1,052,839 - MMP_RS05485 0.90 -0.1 1,052,905 + -0.1 1,053,210 + MMP_RS05490 0.34 -0.6 1,053,327 - MMP_RS05490 0.53 -0.3 1,053,376 - MMP_RS05490 0.61 +0.3 1,053,416 - MMP_RS05490 0.68 -0.3 1,053,458 + MMP_RS05490 0.75 -0.5 1,053,463 - MMP_RS05490 0.76 +0.2 1,053,500 - MMP_RS05490 0.82 +0.3 1,053,599 - -1.0 1,053,606 - +0.4 1,053,656 + +0.0 1,053,658 + -1.3 1,053,673 + -0.2 1,053,706 + -0.3 1,053,713 + -0.4 1,053,740 + -0.3 1,053,944 - MMP_RS05495 0.16 +0.1 1,054,235 + MMP_RS05495 0.32 +0.7 1,054,248 - MMP_RS05495 0.33 -0.4 1,054,272 + MMP_RS05495 0.34 -0.3 1,054,324 + MMP_RS05495 0.37 -0.1 1,054,367 + MMP_RS05495 0.39 -0.3 1,054,425 - MMP_RS05495 0.43 -0.4 1,054,517 - MMP_RS05495 0.48 -1.8 1,054,520 - MMP_RS05495 0.48 -0.1 1,054,620 - MMP_RS05495 0.53 -1.3 1,054,636 - MMP_RS05495 0.54 -1.2 1,054,669 + MMP_RS05495 0.56 -0.2 1,054,737 - MMP_RS05495 0.60 -0.7 1,054,936 + MMP_RS05495 0.70 +0.5 1,054,936 + MMP_RS05495 0.70 -0.3 1,055,011 + MMP_RS05495 0.74 +0.3 1,055,011 + MMP_RS05495 0.74 -1.2 1,055,012 - MMP_RS05495 0.74 -0.4 1,055,060 - MMP_RS05495 0.77 -0.1 1,055,060 - MMP_RS05495 0.77 -2.4 1,055,060 - MMP_RS05495 0.77 +0.4 1,055,061 + MMP_RS05495 0.77 +0.2 1,055,061 + MMP_RS05495 0.77 +0.1 1,055,061 + MMP_RS05495 0.77 +0.2 1,055,072 + MMP_RS05495 0.78 -0.1 1,055,083 + MMP_RS05495 0.78 -0.3 1,055,269 + MMP_RS05495 0.88 -0.4 1,055,409 - +0.2 1,055,409 - -0.3 1,055,414 + +0.2 1,055,421 + -0.1 1,055,424 - +0.3 1,055,424 - -2.0 1,055,458 - +0.1 1,055,458 - +0.2 1,055,459 + +0.0 1,055,459 + +0.6 1,055,459 + +0.5 1,055,459 + +0.0 1,055,459 + +0.3 1,055,477 + -1.1 1,055,523 - +0.1 1,055,525 - -0.6 1,055,561 - +0.5 1,055,561 - -0.4 1,055,576 + +0.0 1,055,985 - MMP_RS05500 0.27 -0.2 1,055,997 + MMP_RS05500 0.28 +0.1 1,056,012 + MMP_RS05500 0.29 -0.5 1,056,273 + MMP_RS05500 0.46 -0.6 1,056,284 - MMP_RS05500 0.46 +0.3 1,056,342 + MMP_RS05500 0.50 +0.1 1,056,361 + MMP_RS05500 0.51 +0.9 1,056,371 - MMP_RS05500 0.52 -0.2
Or see this region's nucleotide sequence