Experiment: H2 N-free with 10mM L-Cysteine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04140 and MMP_RS04145 are separated by 123 nucleotides MMP_RS04145 and MMP_RS04150 are separated by 124 nucleotides MMP_RS04150 and MMP_RS04155 are separated by 58 nucleotides
MMP_RS04140: MMP_RS04140 - phospholipid methyltransferase, at 786,821 to 787,339
_RS04140
MMP_RS04145: MMP_RS04145 - hypothetical protein, at 787,463 to 787,987
_RS04145
MMP_RS04150: MMP_RS04150 - MFS transporter, at 788,112 to 789,326
_RS04150
MMP_RS04155: MMP_RS04155 - MFS transporter, at 789,385 to 790,590
_RS04155
Position (kb)
788
789
790 Strain fitness (log2 ratio)
-1
0
1
2 at 787.129 kb on + strand, within MMP_RS04140 at 787.131 kb on + strand, within MMP_RS04140 at 787.164 kb on + strand, within MMP_RS04140 at 787.180 kb on + strand, within MMP_RS04140 at 787.180 kb on + strand, within MMP_RS04140 at 787.228 kb on + strand, within MMP_RS04140 at 787.256 kb on + strand, within MMP_RS04140 at 787.301 kb on - strand at 787.301 kb on - strand at 787.310 kb on - strand at 787.311 kb on + strand at 787.312 kb on - strand at 787.319 kb on + strand at 787.323 kb on - strand at 787.333 kb on - strand at 787.344 kb on - strand at 787.347 kb on + strand at 787.350 kb on + strand at 787.387 kb on - strand at 787.387 kb on - strand at 787.440 kb on - strand at 787.446 kb on + strand at 787.648 kb on + strand, within MMP_RS04145 at 787.940 kb on + strand at 787.991 kb on - strand at 787.991 kb on - strand at 787.992 kb on + strand at 788.069 kb on - strand at 788.069 kb on - strand at 788.070 kb on + strand at 788.070 kb on + strand at 788.070 kb on + strand at 788.070 kb on + strand at 788.070 kb on + strand at 788.071 kb on - strand at 788.071 kb on - strand at 788.071 kb on - strand at 788.072 kb on + strand at 788.078 kb on - strand at 788.108 kb on - strand at 788.138 kb on + strand at 788.225 kb on + strand at 788.242 kb on - strand, within MMP_RS04150 at 788.247 kb on + strand, within MMP_RS04150 at 788.354 kb on - strand, within MMP_RS04150 at 788.354 kb on - strand, within MMP_RS04150 at 788.404 kb on - strand, within MMP_RS04150 at 788.405 kb on + strand, within MMP_RS04150 at 788.468 kb on - strand, within MMP_RS04150 at 788.612 kb on - strand, within MMP_RS04150 at 788.821 kb on - strand, within MMP_RS04150 at 788.825 kb on - strand, within MMP_RS04150 at 788.825 kb on - strand, within MMP_RS04150 at 788.829 kb on - strand, within MMP_RS04150 at 788.830 kb on + strand, within MMP_RS04150 at 788.863 kb on - strand, within MMP_RS04150 at 789.056 kb on - strand, within MMP_RS04150 at 789.057 kb on + strand, within MMP_RS04150 at 789.230 kb on - strand at 789.311 kb on - strand at 789.324 kb on - strand at 789.334 kb on + strand at 789.339 kb on - strand at 789.340 kb on + strand at 789.340 kb on + strand at 789.340 kb on + strand at 789.340 kb on + strand at 789.404 kb on - strand at 789.404 kb on - strand at 789.429 kb on + strand at 789.490 kb on + strand at 789.496 kb on - strand at 789.497 kb on + strand at 789.511 kb on + strand, within MMP_RS04155 at 789.516 kb on - strand, within MMP_RS04155 at 789.517 kb on + strand, within MMP_RS04155 at 789.517 kb on + strand, within MMP_RS04155 at 789.550 kb on - strand, within MMP_RS04155 at 789.551 kb on + strand, within MMP_RS04155 at 789.551 kb on + strand, within MMP_RS04155 at 789.558 kb on + strand, within MMP_RS04155 at 789.566 kb on - strand, within MMP_RS04155 at 789.567 kb on + strand, within MMP_RS04155 at 789.567 kb on + strand, within MMP_RS04155 at 789.631 kb on + strand, within MMP_RS04155 at 789.662 kb on - strand, within MMP_RS04155 at 789.663 kb on + strand, within MMP_RS04155 at 789.663 kb on + strand, within MMP_RS04155 at 789.670 kb on - strand, within MMP_RS04155 at 789.671 kb on + strand, within MMP_RS04155 at 789.747 kb on - strand, within MMP_RS04155 at 789.748 kb on + strand, within MMP_RS04155 at 789.748 kb on + strand, within MMP_RS04155 at 789.754 kb on - strand, within MMP_RS04155 at 789.754 kb on - strand, within MMP_RS04155 at 789.754 kb on - strand, within MMP_RS04155 at 789.755 kb on + strand, within MMP_RS04155 at 789.755 kb on + strand, within MMP_RS04155 at 789.755 kb on + strand, within MMP_RS04155 at 789.757 kb on + strand, within MMP_RS04155 at 789.894 kb on + strand, within MMP_RS04155 at 789.895 kb on - strand, within MMP_RS04155 at 789.896 kb on + strand, within MMP_RS04155 at 789.896 kb on + strand, within MMP_RS04155 at 789.897 kb on - strand, within MMP_RS04155 at 789.897 kb on - strand, within MMP_RS04155 at 789.897 kb on - strand, within MMP_RS04155 at 789.903 kb on + strand, within MMP_RS04155 at 790.123 kb on + strand, within MMP_RS04155 at 790.228 kb on + strand, within MMP_RS04155 at 790.266 kb on - strand, within MMP_RS04155 at 790.270 kb on - strand, within MMP_RS04155
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Cysteine remove 787,129 + MMP_RS04140 0.59 +0.2 787,131 + MMP_RS04140 0.60 +0.1 787,164 + MMP_RS04140 0.66 -0.1 787,180 + MMP_RS04140 0.69 +0.1 787,180 + MMP_RS04140 0.69 -0.6 787,228 + MMP_RS04140 0.78 +0.2 787,256 + MMP_RS04140 0.84 +0.4 787,301 - +0.1 787,301 - -0.4 787,310 - -0.0 787,311 + +0.5 787,312 - +0.3 787,319 + -1.2 787,323 - +1.4 787,333 - +0.1 787,344 - +0.2 787,347 + -0.0 787,350 + -0.1 787,387 - +0.3 787,387 - -0.4 787,440 - +0.3 787,446 + -0.1 787,648 + MMP_RS04145 0.35 +0.1 787,940 + +0.1 787,991 - -0.6 787,991 - -0.3 787,992 + -0.7 788,069 - -0.5 788,069 - +0.4 788,070 + -0.3 788,070 + -0.8 788,070 + +0.4 788,070 + -0.1 788,070 + +0.1 788,071 - +1.2 788,071 - -0.2 788,071 - +0.2 788,072 + -0.0 788,078 - +0.0 788,108 - -0.1 788,138 + -0.5 788,225 + -0.3 788,242 - MMP_RS04150 0.11 -0.4 788,247 + MMP_RS04150 0.11 -0.6 788,354 - MMP_RS04150 0.20 +0.2 788,354 - MMP_RS04150 0.20 +1.1 788,404 - MMP_RS04150 0.24 -0.6 788,405 + MMP_RS04150 0.24 -0.0 788,468 - MMP_RS04150 0.29 -0.9 788,612 - MMP_RS04150 0.41 -0.1 788,821 - MMP_RS04150 0.58 -0.7 788,825 - MMP_RS04150 0.59 -1.0 788,825 - MMP_RS04150 0.59 -0.0 788,829 - MMP_RS04150 0.59 -0.5 788,830 + MMP_RS04150 0.59 -0.0 788,863 - MMP_RS04150 0.62 -0.5 789,056 - MMP_RS04150 0.78 -0.3 789,057 + MMP_RS04150 0.78 -0.1 789,230 - +0.1 789,311 - -1.3 789,324 - -0.5 789,334 + +0.0 789,339 - -0.1 789,340 + -0.1 789,340 + -0.4 789,340 + +0.7 789,340 + -0.2 789,404 - -0.1 789,404 - -0.0 789,429 + -0.1 789,490 + +0.1 789,496 - +1.5 789,497 + -0.2 789,511 + MMP_RS04155 0.10 +0.1 789,516 - MMP_RS04155 0.11 -0.3 789,517 + MMP_RS04155 0.11 +0.0 789,517 + MMP_RS04155 0.11 -0.4 789,550 - MMP_RS04155 0.14 +0.1 789,551 + MMP_RS04155 0.14 +0.1 789,551 + MMP_RS04155 0.14 +0.4 789,558 + MMP_RS04155 0.14 -0.0 789,566 - MMP_RS04155 0.15 -0.4 789,567 + MMP_RS04155 0.15 -0.3 789,567 + MMP_RS04155 0.15 +0.2 789,631 + MMP_RS04155 0.20 -0.5 789,662 - MMP_RS04155 0.23 +0.4 789,663 + MMP_RS04155 0.23 -0.0 789,663 + MMP_RS04155 0.23 -0.4 789,670 - MMP_RS04155 0.24 -0.1 789,671 + MMP_RS04155 0.24 -0.5 789,747 - MMP_RS04155 0.30 +0.2 789,748 + MMP_RS04155 0.30 -0.3 789,748 + MMP_RS04155 0.30 -0.1 789,754 - MMP_RS04155 0.31 +0.2 789,754 - MMP_RS04155 0.31 +0.2 789,754 - MMP_RS04155 0.31 +0.3 789,755 + MMP_RS04155 0.31 +0.3 789,755 + MMP_RS04155 0.31 -0.1 789,755 + MMP_RS04155 0.31 +0.8 789,757 + MMP_RS04155 0.31 +0.0 789,894 + MMP_RS04155 0.42 +0.1 789,895 - MMP_RS04155 0.42 +0.2 789,896 + MMP_RS04155 0.42 +0.5 789,896 + MMP_RS04155 0.42 +0.4 789,897 - MMP_RS04155 0.42 +0.5 789,897 - MMP_RS04155 0.42 -0.8 789,897 - MMP_RS04155 0.42 -1.0 789,903 + MMP_RS04155 0.43 -0.0 790,123 + MMP_RS04155 0.61 +0.3 790,228 + MMP_RS04155 0.70 -0.5 790,266 - MMP_RS04155 0.73 +2.0 790,270 - MMP_RS04155 0.73 +0.1
Or see this region's nucleotide sequence