Experiment: H2 N-free with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05015 and MMP_RS05020 are separated by 33 nucleotides MMP_RS05020 and MMP_RS05025 are separated by 28 nucleotides MMP_RS05025 and MMP_RS05030 are separated by 21 nucleotides
MMP_RS05015: MMP_RS05015 - histidinol dehydrogenase, at 954,600 to 955,874
_RS05015
MMP_RS05020: MMP_RS05020 - hypothetical protein, at 955,908 to 956,324
_RS05020
MMP_RS05025: MMP_RS05025 - ATP-dependent DNA ligase, at 956,353 to 958,074
_RS05025
MMP_RS05030: MMP_RS05030 - adenylosuccinate lyase, at 958,096 to 959,445
_RS05030
Position (kb) 956
957
958
959 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 955.394 kb on - strand at 955.394 kb on - strand at 955.401 kb on + strand at 955.554 kb on - strand at 955.927 kb on - strand at 955.932 kb on + strand at 956.053 kb on - strand, within MMP_RS05020 at 956.131 kb on - strand, within MMP_RS05020 at 956.174 kb on + strand, within MMP_RS05020 at 956.281 kb on - strand, within MMP_RS05020 at 956.281 kb on - strand, within MMP_RS05020 at 956.299 kb on - strand at 956.661 kb on - strand at 956.695 kb on + strand at 956.893 kb on - strand at 957.050 kb on - strand at 957.333 kb on - strand at 957.463 kb on + strand at 957.657 kb on - strand at 957.657 kb on - strand at 957.772 kb on + strand at 957.901 kb on + strand at 958.020 kb on - strand at 958.023 kb on - strand at 958.023 kb on - strand at 958.029 kb on + strand at 958.055 kb on - strand at 958.055 kb on - strand at 958.072 kb on - strand at 958.093 kb on - strand at 958.119 kb on - strand at 958.381 kb on - strand at 958.615 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Threonine remove 955,394 - -2.2 955,394 - +0.1 955,401 + -0.4 955,554 - -0.2 955,927 - -0.4 955,932 + -0.2 956,053 - MMP_RS05020 0.35 -0.9 956,131 - MMP_RS05020 0.53 -1.9 956,174 + MMP_RS05020 0.64 -1.2 956,281 - MMP_RS05020 0.89 -2.8 956,281 - MMP_RS05020 0.89 -1.4 956,299 - +0.9 956,661 - -1.0 956,695 + -2.9 956,893 - +1.0 957,050 - -2.0 957,333 - -0.7 957,463 + -2.1 957,657 - -2.2 957,657 - +1.3 957,772 + -2.0 957,901 + -1.5 958,020 - -0.9 958,023 - -0.7 958,023 - -0.9 958,029 + +2.1 958,055 - -1.9 958,055 - -0.1 958,072 - -1.3 958,093 - -2.7 958,119 - -1.7 958,381 - +1.9 958,615 + +0.0
Or see this region's nucleotide sequence