Experiment: H2 N-free with 10mM L-Threonine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04640 and MMP_RS04645 are separated by 22 nucleotides MMP_RS04645 and MMP_RS04650 are separated by 123 nucleotides MMP_RS04650 and MMP_RS04655 are separated by 21 nucleotides
MMP_RS04640: MMP_RS04640 - CTP synthase (glutamine hydrolyzing), at 885,559 to 887,160
_RS04640
MMP_RS04645: MMP_RS04645 - glutamine-hydrolyzing GMP synthase, at 887,183 to 888,115
_RS04645
MMP_RS04650: MMP_RS04650 - tetratricopeptide repeat protein, at 888,239 to 888,832
_RS04650
MMP_RS04655: MMP_RS04655 - flippase, at 888,854 to 890,125
_RS04655
Position (kb) 887
888
889 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 886.349 kb on - strand at 886.474 kb on + strand at 887.953 kb on - strand at 888.152 kb on + strand at 888.155 kb on - strand at 888.156 kb on + strand at 888.162 kb on - strand at 888.162 kb on - strand at 888.162 kb on - strand at 888.163 kb on + strand at 888.166 kb on - strand at 888.167 kb on + strand at 888.167 kb on + strand at 888.193 kb on - strand at 888.193 kb on - strand at 888.194 kb on + strand at 888.194 kb on + strand at 888.194 kb on + strand at 888.292 kb on - strand at 888.458 kb on - strand, within MMP_RS04650 at 888.472 kb on + strand, within MMP_RS04650 at 888.489 kb on - strand, within MMP_RS04650 at 888.524 kb on - strand, within MMP_RS04650 at 888.524 kb on - strand, within MMP_RS04650 at 888.526 kb on - strand, within MMP_RS04650 at 888.586 kb on - strand, within MMP_RS04650 at 888.612 kb on - strand, within MMP_RS04650 at 888.643 kb on - strand, within MMP_RS04650 at 888.653 kb on + strand, within MMP_RS04650 at 888.667 kb on + strand, within MMP_RS04650 at 888.684 kb on - strand, within MMP_RS04650 at 888.695 kb on + strand, within MMP_RS04650 at 888.713 kb on - strand, within MMP_RS04650 at 888.713 kb on - strand, within MMP_RS04650 at 888.714 kb on + strand, within MMP_RS04650 at 888.714 kb on + strand, within MMP_RS04650 at 888.714 kb on + strand, within MMP_RS04650 at 888.868 kb on + strand at 888.899 kb on - strand at 888.899 kb on - strand at 888.900 kb on + strand at 888.900 kb on + strand at 888.900 kb on + strand at 888.920 kb on - strand at 888.965 kb on + strand at 888.990 kb on - strand at 889.015 kb on + strand at 889.023 kb on - strand at 889.024 kb on + strand at 889.106 kb on - strand at 889.112 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Threonine remove 886,349 - +1.1 886,474 + -1.5 887,953 - -1.2 888,152 + -0.4 888,155 - +0.6 888,156 + +1.1 888,162 - +0.3 888,162 - -0.7 888,162 - +3.3 888,163 + +0.2 888,166 - -1.2 888,167 + +1.0 888,167 + +2.0 888,193 - +0.6 888,193 - -1.5 888,194 + -1.1 888,194 + -0.5 888,194 + -0.0 888,292 - -0.4 888,458 - MMP_RS04650 0.37 -1.1 888,472 + MMP_RS04650 0.39 +2.1 888,489 - MMP_RS04650 0.42 -1.1 888,524 - MMP_RS04650 0.48 -0.6 888,524 - MMP_RS04650 0.48 +0.5 888,526 - MMP_RS04650 0.48 +0.4 888,586 - MMP_RS04650 0.58 -2.0 888,612 - MMP_RS04650 0.63 -0.3 888,643 - MMP_RS04650 0.68 -0.1 888,653 + MMP_RS04650 0.70 -2.1 888,667 + MMP_RS04650 0.72 -0.8 888,684 - MMP_RS04650 0.75 -1.1 888,695 + MMP_RS04650 0.77 -1.2 888,713 - MMP_RS04650 0.80 +0.2 888,713 - MMP_RS04650 0.80 -0.3 888,714 + MMP_RS04650 0.80 +1.2 888,714 + MMP_RS04650 0.80 -0.7 888,714 + MMP_RS04650 0.80 +0.1 888,868 + +0.4 888,899 - -2.4 888,899 - +0.3 888,900 + +2.2 888,900 + -1.5 888,900 + +0.5 888,920 - -2.9 888,965 + -0.5 888,990 - -1.0 889,015 + -1.9 889,023 - -0.7 889,024 + -0.7 889,106 - +0.1 889,112 + +0.9
Or see this region's nucleotide sequence