Experiment: H2 N-free with 10mM L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS03335 and MMP_RS03340 are separated by 2 nucleotides MMP_RS03340 and MMP_RS03345 are separated by 213 nucleotides MMP_RS03345 and MMP_RS03350 are separated by 39 nucleotides MMP_RS03350 and MMP_RS03355 are separated by 108 nucleotides
MMP_RS03335: MMP_RS03335 - ABC transporter permease, at 626,477 to 627,670
_RS03335
MMP_RS03340: MMP_RS03340 - ABC transporter ATP-binding protein, at 627,673 to 628,398
_RS03340
MMP_RS03345: MMP_RS03345 - hypothetical protein, at 628,612 to 629,844
_RS03345
MMP_RS03350: MMP_RS03350 - winged helix-turn-helix domain-containing protein, at 629,884 to 630,426
_RS03350
MMP_RS03355: MMP_RS03355 - beta-propeller domain-containing protein, at 630,535 to 632,772
_RS03355
Position (kb) 628
629
630 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 627.653 kb on + strand at 627.726 kb on - strand at 627.971 kb on - strand, within MMP_RS03340 at 628.394 kb on - strand at 628.441 kb on + strand at 628.506 kb on - strand at 628.544 kb on - strand at 628.551 kb on - strand at 628.559 kb on - strand at 628.559 kb on - strand at 628.561 kb on - strand at 628.579 kb on - strand at 628.745 kb on - strand, within MMP_RS03345 at 628.746 kb on + strand, within MMP_RS03345 at 628.746 kb on + strand, within MMP_RS03345 at 628.795 kb on - strand, within MMP_RS03345 at 628.800 kb on - strand, within MMP_RS03345 at 628.817 kb on - strand, within MMP_RS03345 at 628.999 kb on - strand, within MMP_RS03345 at 629.048 kb on - strand, within MMP_RS03345 at 629.049 kb on + strand, within MMP_RS03345 at 629.053 kb on - strand, within MMP_RS03345 at 629.063 kb on - strand, within MMP_RS03345 at 629.064 kb on + strand, within MMP_RS03345 at 629.065 kb on - strand, within MMP_RS03345 at 629.065 kb on - strand, within MMP_RS03345 at 629.065 kb on - strand, within MMP_RS03345 at 629.141 kb on - strand, within MMP_RS03345 at 629.141 kb on - strand, within MMP_RS03345 at 629.142 kb on + strand, within MMP_RS03345 at 629.143 kb on - strand, within MMP_RS03345 at 629.143 kb on - strand, within MMP_RS03345 at 629.144 kb on + strand, within MMP_RS03345 at 629.144 kb on + strand, within MMP_RS03345 at 629.366 kb on + strand, within MMP_RS03345 at 629.368 kb on + strand, within MMP_RS03345 at 629.557 kb on - strand, within MMP_RS03345 at 629.569 kb on + strand, within MMP_RS03345 at 629.608 kb on - strand, within MMP_RS03345 at 629.674 kb on - strand, within MMP_RS03345 at 629.754 kb on - strand at 629.760 kb on - strand at 629.768 kb on - strand at 629.790 kb on + strand at 629.801 kb on - strand at 629.802 kb on + strand at 629.802 kb on + strand at 629.804 kb on + strand at 629.805 kb on - strand at 629.845 kb on - strand at 629.883 kb on - strand at 629.952 kb on + strand, within MMP_RS03350 at 630.202 kb on + strand, within MMP_RS03350 at 630.203 kb on - strand, within MMP_RS03350 at 630.322 kb on + strand, within MMP_RS03350 at 630.407 kb on - strand at 630.412 kb on - strand at 630.413 kb on + strand at 630.483 kb on - strand at 630.575 kb on - strand at 630.599 kb on + strand at 630.599 kb on + strand at 630.628 kb on - strand at 630.628 kb on - strand at 630.631 kb on + strand at 630.713 kb on + strand at 630.730 kb on + strand at 630.794 kb on + strand, within MMP_RS03355
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Serine remove 627,653 + -0.3 627,726 - -0.8 627,971 - MMP_RS03340 0.41 -2.3 628,394 - +4.3 628,441 + -0.9 628,506 - +0.2 628,544 - +1.1 628,551 - -0.5 628,559 - -0.6 628,559 - +0.7 628,561 - -0.6 628,579 - -2.0 628,745 - MMP_RS03345 0.11 -1.3 628,746 + MMP_RS03345 0.11 +0.2 628,746 + MMP_RS03345 0.11 +0.1 628,795 - MMP_RS03345 0.15 -0.2 628,800 - MMP_RS03345 0.15 -0.0 628,817 - MMP_RS03345 0.17 -1.6 628,999 - MMP_RS03345 0.31 -0.3 629,048 - MMP_RS03345 0.35 +0.2 629,049 + MMP_RS03345 0.35 -0.4 629,053 - MMP_RS03345 0.36 +0.1 629,063 - MMP_RS03345 0.37 -0.5 629,064 + MMP_RS03345 0.37 -0.7 629,065 - MMP_RS03345 0.37 +0.3 629,065 - MMP_RS03345 0.37 +0.5 629,065 - MMP_RS03345 0.37 +0.5 629,141 - MMP_RS03345 0.43 -0.8 629,141 - MMP_RS03345 0.43 -0.5 629,142 + MMP_RS03345 0.43 -0.4 629,143 - MMP_RS03345 0.43 +0.0 629,143 - MMP_RS03345 0.43 -0.2 629,144 + MMP_RS03345 0.43 -0.6 629,144 + MMP_RS03345 0.43 -2.1 629,366 + MMP_RS03345 0.61 +0.5 629,368 + MMP_RS03345 0.61 -0.5 629,557 - MMP_RS03345 0.77 -0.2 629,569 + MMP_RS03345 0.78 -0.5 629,608 - MMP_RS03345 0.81 -0.0 629,674 - MMP_RS03345 0.86 -0.5 629,754 - +1.0 629,760 - -2.6 629,768 - +0.5 629,790 + +0.6 629,801 - -1.3 629,802 + -1.7 629,802 + +0.4 629,804 + +0.8 629,805 - +0.1 629,845 - -0.2 629,883 - -0.8 629,952 + MMP_RS03350 0.13 -0.4 630,202 + MMP_RS03350 0.59 -1.6 630,203 - MMP_RS03350 0.59 +0.9 630,322 + MMP_RS03350 0.81 +0.1 630,407 - -0.7 630,412 - +0.2 630,413 + +0.5 630,483 - -1.2 630,575 - -1.2 630,599 + -0.6 630,599 + -2.4 630,628 - -0.8 630,628 - -0.1 630,631 + -0.7 630,713 + +0.4 630,730 + -0.5 630,794 + MMP_RS03355 0.12 -0.6
Or see this region's nucleotide sequence