Experiment: H2 N-free with 10mM L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS02530 and MMP_RS02535 overlap by 4 nucleotides MMP_RS02535 and MMP_RS02540 are separated by 148 nucleotides MMP_RS02540 and MMP_RS02545 are separated by 586 nucleotides
MMP_RS02530: MMP_RS02530 - type II toxin-antitoxin system mRNA interferase toxin RelE/StbE family, at 473,717 to 474,019
_RS02530
MMP_RS02535: MMP_RS02535 - DUF2683 family protein, at 474,016 to 474,264
_RS02535
MMP_RS02540: MMP_RS02540 - DUF2283 domain-containing protein, at 474,413 to 474,790
_RS02540
MMP_RS02545: MMP_RS02545 - hypothetical protein, at 475,377 to 475,787
_RS02545
Position (kb) 474
475 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 473.445 kb on + strand at 473.450 kb on - strand at 473.460 kb on - strand at 473.650 kb on + strand at 473.754 kb on - strand, within MMP_RS02530 at 473.755 kb on + strand, within MMP_RS02530 at 473.776 kb on + strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.787 kb on - strand, within MMP_RS02530 at 473.788 kb on + strand, within MMP_RS02530 at 473.789 kb on - strand, within MMP_RS02530 at 473.838 kb on - strand, within MMP_RS02530 at 473.948 kb on + strand, within MMP_RS02530 at 473.989 kb on - strand at 474.169 kb on - strand at 474.284 kb on + strand at 474.306 kb on - strand at 474.319 kb on - strand at 474.323 kb on - strand at 474.324 kb on + strand at 474.367 kb on + strand at 474.368 kb on - strand at 474.369 kb on + strand at 474.374 kb on - strand at 474.375 kb on + strand at 474.657 kb on + strand, within MMP_RS02540 at 474.714 kb on - strand, within MMP_RS02540 at 474.742 kb on + strand, within MMP_RS02540 at 474.742 kb on + strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.746 kb on - strand, within MMP_RS02540 at 474.747 kb on + strand, within MMP_RS02540 at 474.766 kb on + strand at 474.814 kb on - strand at 474.814 kb on - strand at 474.839 kb on - strand at 474.840 kb on + strand at 474.841 kb on - strand at 474.841 kb on - strand at 474.846 kb on - strand at 474.847 kb on + strand at 474.847 kb on + strand at 474.852 kb on + strand at 474.880 kb on + strand at 474.883 kb on - strand at 474.900 kb on + strand at 475.037 kb on - strand at 475.271 kb on - strand at 475.350 kb on - strand at 475.413 kb on + strand at 475.414 kb on - strand at 475.415 kb on + strand at 475.459 kb on + strand, within MMP_RS02545 at 475.480 kb on + strand, within MMP_RS02545 at 475.504 kb on + strand, within MMP_RS02545 at 475.548 kb on - strand, within MMP_RS02545 at 475.605 kb on + strand, within MMP_RS02545 at 475.710 kb on - strand, within MMP_RS02545 at 475.724 kb on + strand, within MMP_RS02545
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Serine remove 473,445 + -0.9 473,450 - +0.6 473,460 - -0.2 473,650 + +0.1 473,754 - MMP_RS02530 0.12 +0.4 473,755 + MMP_RS02530 0.13 -1.2 473,776 + MMP_RS02530 0.19 +0.1 473,787 - MMP_RS02530 0.23 -1.2 473,787 - MMP_RS02530 0.23 -0.3 473,787 - MMP_RS02530 0.23 -1.2 473,788 + MMP_RS02530 0.23 -0.0 473,789 - MMP_RS02530 0.24 +0.8 473,838 - MMP_RS02530 0.40 +0.1 473,948 + MMP_RS02530 0.76 -1.0 473,989 - -0.9 474,169 - +1.0 474,284 + +0.6 474,306 - +3.4 474,319 - +4.5 474,323 - +0.4 474,324 + +0.3 474,367 + +0.4 474,368 - +0.6 474,369 + +0.3 474,374 - +0.7 474,375 + +1.5 474,657 + MMP_RS02540 0.65 +0.6 474,714 - MMP_RS02540 0.80 -2.1 474,742 + MMP_RS02540 0.87 -0.4 474,742 + MMP_RS02540 0.87 -1.0 474,746 - MMP_RS02540 0.88 -2.3 474,746 - MMP_RS02540 0.88 +0.2 474,746 - MMP_RS02540 0.88 -0.5 474,747 + MMP_RS02540 0.88 +0.4 474,766 + -0.2 474,814 - -0.2 474,814 - +0.3 474,839 - -1.1 474,840 + -0.4 474,841 - +0.1 474,841 - +0.7 474,846 - -0.0 474,847 + -0.3 474,847 + -0.5 474,852 + +0.3 474,880 + -0.4 474,883 - +0.1 474,900 + -1.3 475,037 - +0.6 475,271 - -0.6 475,350 - -0.1 475,413 + -1.0 475,414 - -0.4 475,415 + -0.4 475,459 + MMP_RS02545 0.20 +0.2 475,480 + MMP_RS02545 0.25 -1.5 475,504 + MMP_RS02545 0.31 +0.2 475,548 - MMP_RS02545 0.42 -0.7 475,605 + MMP_RS02545 0.55 -0.4 475,710 - MMP_RS02545 0.81 -1.7 475,724 + MMP_RS02545 0.84 +0.3
Or see this region's nucleotide sequence