Experiment: H2 N-free with 10mM L-Serine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01670 and MMP_RS01675 are separated by 8 nucleotides MMP_RS01675 and MMP_RS01680 are separated by 314 nucleotides MMP_RS01680 and MMP_RS01685 overlap by 8 nucleotides MMP_RS01685 and MMP_RS01690 overlap by 4 nucleotides
MMP_RS01670: MMP_RS01670 - methanogenesis marker 5 protein, at 307,706 to 308,146
_RS01670
MMP_RS01675: MMP_RS01675 - hypothetical protein, at 308,155 to 308,610
_RS01675
MMP_RS01680: MMP_RS01680 - phenylacetate--CoA ligase, at 308,925 to 310,169
_RS01680
MMP_RS01685: MMP_RS01685 - indolepyruvate oxidoreductase subunit beta, at 310,162 to 310,761
_RS01685
MMP_RS01690: MMP_RS01690 - indolepyruvate ferredoxin oxidoreductase subunit alpha, at 310,758 to 312,596
_RS01690
Position (kb) 308
309
310
311 Strain fitness (log2 ratio) -2
-1
0
1
2 at 308.172 kb on - strand at 308.340 kb on + strand, within MMP_RS01675 at 308.592 kb on + strand at 308.592 kb on + strand at 308.595 kb on + strand at 308.612 kb on - strand at 308.613 kb on + strand at 308.613 kb on + strand at 308.614 kb on - strand at 308.615 kb on + strand at 308.671 kb on - strand at 308.671 kb on - strand at 308.672 kb on + strand at 308.674 kb on + strand at 308.676 kb on + strand at 308.677 kb on - strand at 308.690 kb on - strand at 308.708 kb on - strand at 308.717 kb on + strand at 308.725 kb on - strand at 308.726 kb on + strand at 308.726 kb on + strand at 308.727 kb on - strand at 308.854 kb on - strand at 309.019 kb on - strand at 309.020 kb on + strand at 309.036 kb on - strand at 309.092 kb on - strand, within MMP_RS01680 at 309.097 kb on + strand, within MMP_RS01680 at 309.110 kb on - strand, within MMP_RS01680 at 309.276 kb on + strand, within MMP_RS01680 at 309.344 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.489 kb on - strand, within MMP_RS01680 at 309.490 kb on + strand, within MMP_RS01680 at 309.509 kb on - strand, within MMP_RS01680 at 309.821 kb on - strand, within MMP_RS01680 at 309.828 kb on + strand, within MMP_RS01680 at 309.879 kb on + strand, within MMP_RS01680 at 309.882 kb on - strand, within MMP_RS01680 at 309.883 kb on + strand, within MMP_RS01680 at 309.898 kb on - strand, within MMP_RS01680 at 309.903 kb on - strand, within MMP_RS01680 at 309.943 kb on + strand, within MMP_RS01680 at 310.049 kb on - strand at 310.050 kb on + strand at 310.065 kb on - strand at 310.065 kb on - strand at 310.065 kb on - strand at 310.397 kb on - strand, within MMP_RS01685 at 310.486 kb on - strand, within MMP_RS01685 at 310.745 kb on + strand at 310.997 kb on + strand, within MMP_RS01690 at 311.111 kb on - strand, within MMP_RS01690
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Serine remove 308,172 - -0.2 308,340 + MMP_RS01675 0.41 +0.5 308,592 + +0.5 308,592 + +0.8 308,595 + +1.0 308,612 - +0.3 308,613 + -1.3 308,613 + +1.6 308,614 - -0.5 308,615 + +0.2 308,671 - -0.1 308,671 - -0.4 308,672 + -0.1 308,674 + +1.0 308,676 + -1.1 308,677 - -1.4 308,690 - +0.7 308,708 - -0.6 308,717 + +0.6 308,725 - -0.8 308,726 + +1.8 308,726 + +0.8 308,727 - +0.6 308,854 - +0.0 309,019 - +0.4 309,020 + +0.0 309,036 - +0.4 309,092 - MMP_RS01680 0.13 +0.4 309,097 + MMP_RS01680 0.14 +0.0 309,110 - MMP_RS01680 0.15 -0.1 309,276 + MMP_RS01680 0.28 +1.0 309,344 - MMP_RS01680 0.34 -0.4 309,489 - MMP_RS01680 0.45 -0.4 309,489 - MMP_RS01680 0.45 +0.1 309,489 - MMP_RS01680 0.45 -1.8 309,490 + MMP_RS01680 0.45 -0.3 309,509 - MMP_RS01680 0.47 +0.2 309,821 - MMP_RS01680 0.72 -1.3 309,828 + MMP_RS01680 0.73 +0.3 309,879 + MMP_RS01680 0.77 -1.1 309,882 - MMP_RS01680 0.77 -0.2 309,883 + MMP_RS01680 0.77 -1.2 309,898 - MMP_RS01680 0.78 -0.1 309,903 - MMP_RS01680 0.79 -0.9 309,943 + MMP_RS01680 0.82 -0.8 310,049 - -2.4 310,050 + +0.3 310,065 - -0.6 310,065 - -1.4 310,065 - +0.6 310,397 - MMP_RS01685 0.39 -0.4 310,486 - MMP_RS01685 0.54 -0.2 310,745 + +0.3 310,997 + MMP_RS01690 0.13 -0.3 311,111 - MMP_RS01690 0.19 +0.1
Or see this region's nucleotide sequence