Experiment: H2 N-free with 10mM L-Glutamic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS06405 and MMP_RS06410 are separated by 22 nucleotides MMP_RS06410 and MMP_RS06415 are separated by 41 nucleotides MMP_RS06415 and MMP_RS06420 are separated by 22 nucleotides
MMP_RS06405: MMP_RS06405 - 4Fe-4S binding protein, at 1,229,443 to 1,230,501
_RS06405
MMP_RS06410: MMP_RS06410 - 4Fe-4S binding protein, at 1,230,524 to 1,230,766
_RS06410
MMP_RS06415: MMP_RS06415 - protein fwdD, at 1,230,808 to 1,231,194
_RS06415
MMP_RS06420: MMP_RS06420 - formylmethanofuran dehydrogenase subunit A, at 1,231,217 to 1,232,920
_RS06420
Position (kb)
1230
1231
1232 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1230.027 kb on + strand at 1230.083 kb on + strand at 1230.357 kb on + strand at 1230.445 kb on + strand at 1230.540 kb on - strand at 1230.541 kb on + strand at 1230.612 kb on - strand at 1230.613 kb on + strand at 1230.685 kb on + strand at 1230.959 kb on - strand at 1231.035 kb on + strand at 1231.170 kb on + strand at 1231.184 kb on + strand at 1231.649 kb on + strand at 1231.834 kb on - strand at 1232.144 kb on + strand at 1232.159 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Glutamic remove 1,230,027 + -2.1 1,230,083 + -1.2 1,230,357 + -4.3 1,230,445 + -3.7 1,230,540 - -3.0 1,230,541 + -4.5 1,230,612 - -3.0 1,230,613 + -1.7 1,230,685 + -3.9 1,230,959 - -3.0 1,231,035 + -3.0 1,231,170 + -2.3 1,231,184 + -3.7 1,231,649 + -3.6 1,231,834 - -2.8 1,232,144 + -2.3 1,232,159 + -3.5
Or see this region's nucleotide sequence