Experiment: H2 N-free with 10mM L-Glutamic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS04550 and MMP_RS04555 are separated by 9 nucleotides MMP_RS04555 and MMP_RS04560 are separated by 22 nucleotides MMP_RS04560 and MMP_RS04565 are separated by 88 nucleotides
MMP_RS04550: MMP_RS04550 - S-layer protein, at 867,911 to 869,512
_RS04550
MMP_RS04555: MMP_RS04555 - 7 8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit CofG, at 869,522 to 870,580
_RS04555
MMP_RS04560: MMP_RS04560 - carboxylating nicotinate-nucleotide diphosphorylase, at 870,603 to 871,442
_RS04560
MMP_RS04565: MMP_RS04565 - ribonuclease P, at 871,531 to 871,923
_RS04565
Position (kb) 869
870
871 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 868.536 kb on - strand, within MMP_RS04550 at 868.562 kb on + strand, within MMP_RS04550 at 868.569 kb on + strand, within MMP_RS04550 at 868.591 kb on + strand, within MMP_RS04550 at 868.671 kb on + strand, within MMP_RS04550 at 868.723 kb on - strand, within MMP_RS04550 at 868.724 kb on + strand, within MMP_RS04550 at 868.727 kb on - strand, within MMP_RS04550 at 868.728 kb on + strand, within MMP_RS04550 at 868.728 kb on + strand, within MMP_RS04550 at 868.730 kb on + strand, within MMP_RS04550 at 868.734 kb on - strand, within MMP_RS04550 at 868.734 kb on - strand, within MMP_RS04550 at 868.735 kb on + strand, within MMP_RS04550 at 868.749 kb on - strand, within MMP_RS04550 at 868.778 kb on - strand, within MMP_RS04550 at 868.846 kb on - strand, within MMP_RS04550 at 868.878 kb on - strand, within MMP_RS04550 at 868.932 kb on + strand, within MMP_RS04550 at 868.950 kb on - strand, within MMP_RS04550 at 868.951 kb on + strand, within MMP_RS04550 at 868.952 kb on - strand, within MMP_RS04550 at 868.953 kb on + strand, within MMP_RS04550 at 868.954 kb on - strand, within MMP_RS04550 at 869.006 kb on + strand, within MMP_RS04550 at 869.024 kb on - strand, within MMP_RS04550 at 869.025 kb on + strand, within MMP_RS04550 at 869.025 kb on + strand, within MMP_RS04550 at 869.062 kb on - strand, within MMP_RS04550 at 869.063 kb on + strand, within MMP_RS04550 at 869.122 kb on - strand, within MMP_RS04550 at 869.122 kb on - strand, within MMP_RS04550 at 869.122 kb on - strand, within MMP_RS04550 at 869.122 kb on - strand, within MMP_RS04550 at 869.122 kb on - strand, within MMP_RS04550 at 869.122 kb on - strand, within MMP_RS04550 at 869.123 kb on + strand, within MMP_RS04550 at 869.123 kb on + strand, within MMP_RS04550 at 869.123 kb on + strand, within MMP_RS04550 at 869.123 kb on + strand, within MMP_RS04550 at 869.133 kb on - strand, within MMP_RS04550 at 869.134 kb on + strand, within MMP_RS04550 at 869.134 kb on + strand, within MMP_RS04550 at 869.167 kb on - strand, within MMP_RS04550 at 869.168 kb on + strand, within MMP_RS04550 at 869.170 kb on + strand, within MMP_RS04550 at 869.223 kb on - strand, within MMP_RS04550 at 869.223 kb on - strand, within MMP_RS04550 at 869.223 kb on - strand, within MMP_RS04550 at 869.253 kb on - strand, within MMP_RS04550 at 869.274 kb on - strand, within MMP_RS04550 at 869.275 kb on + strand, within MMP_RS04550 at 869.320 kb on + strand, within MMP_RS04550 at 869.358 kb on - strand at 869.362 kb on + strand at 869.406 kb on - strand at 869.464 kb on - strand at 869.465 kb on + strand at 869.518 kb on - strand at 869.524 kb on + strand at 869.569 kb on - strand at 869.570 kb on + strand at 869.572 kb on - strand at 869.574 kb on - strand at 869.575 kb on + strand at 869.575 kb on + strand at 869.609 kb on + strand at 869.876 kb on + strand at 869.940 kb on + strand at 869.980 kb on - strand at 869.985 kb on + strand at 869.996 kb on + strand at 870.047 kb on + strand at 870.167 kb on - strand at 870.173 kb on + strand at 870.225 kb on + strand at 870.225 kb on + strand at 870.225 kb on + strand at 870.242 kb on - strand at 870.353 kb on + strand at 870.406 kb on - strand at 870.490 kb on - strand at 870.542 kb on + strand at 870.580 kb on - strand at 870.599 kb on - strand at 870.677 kb on - strand at 870.701 kb on - strand at 870.819 kb on - strand at 870.822 kb on - strand at 870.833 kb on - strand at 870.833 kb on - strand at 870.833 kb on - strand at 870.834 kb on + strand at 870.844 kb on + strand at 871.058 kb on - strand at 871.082 kb on - strand at 871.115 kb on - strand at 871.285 kb on + strand at 871.390 kb on + strand at 871.480 kb on - strand at 871.481 kb on + strand at 871.485 kb on + strand at 871.497 kb on + strand at 871.508 kb on + strand at 871.512 kb on - strand at 871.512 kb on - strand at 871.512 kb on - strand at 871.512 kb on - strand at 871.514 kb on - strand at 871.514 kb on - strand at 871.514 kb on - strand at 871.515 kb on + strand at 871.515 kb on + strand at 871.515 kb on + strand at 871.525 kb on - strand at 871.527 kb on - strand at 871.528 kb on + strand at 871.528 kb on + strand at 871.528 kb on + strand at 871.528 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Glutamic remove 868,536 - MMP_RS04550 0.39 +0.8 868,562 + MMP_RS04550 0.41 +0.8 868,569 + MMP_RS04550 0.41 +1.3 868,591 + MMP_RS04550 0.42 +0.3 868,671 + MMP_RS04550 0.47 +0.4 868,723 - MMP_RS04550 0.51 -0.7 868,724 + MMP_RS04550 0.51 +1.1 868,727 - MMP_RS04550 0.51 +0.0 868,728 + MMP_RS04550 0.51 +0.3 868,728 + MMP_RS04550 0.51 +1.1 868,730 + MMP_RS04550 0.51 +1.4 868,734 - MMP_RS04550 0.51 +2.7 868,734 - MMP_RS04550 0.51 +1.4 868,735 + MMP_RS04550 0.51 -1.0 868,749 - MMP_RS04550 0.52 +0.5 868,778 - MMP_RS04550 0.54 -0.3 868,846 - MMP_RS04550 0.58 +0.4 868,878 - MMP_RS04550 0.60 -0.4 868,932 + MMP_RS04550 0.64 -0.1 868,950 - MMP_RS04550 0.65 +0.8 868,951 + MMP_RS04550 0.65 -0.4 868,952 - MMP_RS04550 0.65 +1.4 868,953 + MMP_RS04550 0.65 -1.1 868,954 - MMP_RS04550 0.65 +1.0 869,006 + MMP_RS04550 0.68 -0.4 869,024 - MMP_RS04550 0.69 -0.2 869,025 + MMP_RS04550 0.70 +0.5 869,025 + MMP_RS04550 0.70 -0.9 869,062 - MMP_RS04550 0.72 +0.7 869,063 + MMP_RS04550 0.72 -1.5 869,122 - MMP_RS04550 0.76 -0.5 869,122 - MMP_RS04550 0.76 +0.3 869,122 - MMP_RS04550 0.76 +0.8 869,122 - MMP_RS04550 0.76 +0.2 869,122 - MMP_RS04550 0.76 -0.1 869,122 - MMP_RS04550 0.76 -1.2 869,123 + MMP_RS04550 0.76 +1.0 869,123 + MMP_RS04550 0.76 +0.8 869,123 + MMP_RS04550 0.76 +0.7 869,123 + MMP_RS04550 0.76 +0.7 869,133 - MMP_RS04550 0.76 -0.2 869,134 + MMP_RS04550 0.76 +1.2 869,134 + MMP_RS04550 0.76 +0.7 869,167 - MMP_RS04550 0.78 -0.5 869,168 + MMP_RS04550 0.78 +1.2 869,170 + MMP_RS04550 0.79 +1.6 869,223 - MMP_RS04550 0.82 +2.9 869,223 - MMP_RS04550 0.82 +1.0 869,223 - MMP_RS04550 0.82 -1.9 869,253 - MMP_RS04550 0.84 -0.5 869,274 - MMP_RS04550 0.85 +0.8 869,275 + MMP_RS04550 0.85 +0.7 869,320 + MMP_RS04550 0.88 +2.2 869,358 - +0.7 869,362 + +1.1 869,406 - -0.2 869,464 - +0.4 869,465 + +0.3 869,518 - +0.5 869,524 + -0.0 869,569 - -2.9 869,570 + -4.8 869,572 - -3.8 869,574 - -3.1 869,575 + -3.2 869,575 + -3.4 869,609 + -3.0 869,876 + -2.4 869,940 + -1.2 869,980 - -1.1 869,985 + -3.4 869,996 + -2.9 870,047 + -2.2 870,167 - -1.1 870,173 + -3.9 870,225 + -1.6 870,225 + -4.8 870,225 + -0.4 870,242 - -3.3 870,353 + -3.2 870,406 - -0.6 870,490 - -3.1 870,542 + -2.7 870,580 - -2.8 870,599 - +1.0 870,677 - -4.4 870,701 - -3.1 870,819 - -2.4 870,822 - -3.9 870,833 - -4.3 870,833 - -3.4 870,833 - -2.3 870,834 + -4.5 870,844 + -3.5 871,058 - -3.0 871,082 - -3.7 871,115 - -2.8 871,285 + -0.1 871,390 + -4.4 871,480 - -1.0 871,481 + -1.4 871,485 + -1.6 871,497 + +1.7 871,508 + +0.1 871,512 - -3.1 871,512 - -5.4 871,512 - -2.5 871,512 - -2.3 871,514 - -1.0 871,514 - -2.2 871,514 - -2.6 871,515 + -1.1 871,515 + -1.9 871,515 + -2.6 871,525 - -3.8 871,527 - -1.5 871,528 + -3.4 871,528 + -1.3 871,528 + -1.4 871,528 + -3.9
Or see this region's nucleotide sequence