Experiment: H2 N-free with 10mM L-Arginine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS05735 and MMP_RS05740 overlap by 14 nucleotides MMP_RS05740 and MMP_RS05745 are separated by 4 nucleotides MMP_RS05745 and MMP_RS05750 are separated by 60 nucleotides
MMP_RS05735: MMP_RS05735 - oligosaccharide repeat unit polymerase family protein, at 1,100,167 to 1,101,342
_RS05735
MMP_RS05740: MMP_RS05740 - oligosaccharide repeat unit polymerase family protein, at 1,101,329 to 1,102,402
_RS05740
MMP_RS05745: MMP_RS05745 - hypothetical protein, at 1,102,407 to 1,103,162
_RS05745
MMP_RS05750: MMP_RS05750 - transketolase family protein, at 1,103,223 to 1,104,161
_RS05750
Position (kb)
1101
1102
1103 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1100.332 kb on - strand at 1100.894 kb on + strand at 1100.960 kb on - strand at 1100.980 kb on - strand at 1101.022 kb on - strand at 1101.366 kb on - strand at 1101.448 kb on + strand at 1101.617 kb on - strand at 1101.897 kb on - strand at 1102.070 kb on - strand at 1102.071 kb on + strand at 1102.576 kb on + strand at 1102.739 kb on - strand at 1102.740 kb on + strand at 1102.740 kb on + strand at 1102.750 kb on + strand at 1102.787 kb on - strand at 1102.830 kb on + strand at 1102.932 kb on - strand at 1103.115 kb on - strand at 1103.195 kb on - strand at 1103.207 kb on + strand at 1103.207 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 N-free with 10mM L-Arginine remove 1,100,332 - -2.9 1,100,894 + +0.5 1,100,960 - +1.9 1,100,980 - -0.5 1,101,022 - -3.4 1,101,366 - -0.9 1,101,448 + -0.1 1,101,617 - -1.1 1,101,897 - -4.3 1,102,070 - -2.1 1,102,071 + -4.0 1,102,576 + -2.5 1,102,739 - -2.7 1,102,740 + -3.1 1,102,740 + +0.3 1,102,750 + +0.4 1,102,787 - -2.4 1,102,830 + -3.9 1,102,932 - -2.2 1,103,115 - -2.7 1,103,195 - +0.6 1,103,207 + -1.1 1,103,207 + -1.2
Or see this region's nucleotide sequence